Development of the analytical method to quantify toxicological substances in formalin-fixed tissue

• Project/Area Number: 15K08874
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Ehime University
• Principal Investigator: Asano Migiwa 愛媛大学, 医学系研究科, 教授 (90283879)
• Co-Investigator(Kenkyū-buntansha): 上野 易弘 神戸大学, 医学研究科, 教授 (30184956)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 薬物中毒 / 薬物定量分析 / ホルマリン固定 / 肝臓 / UPLC-MS/MS / 急性薬物中毒 / ホルマリン固定臓器 / LC-MS/MS

Outline of Final Research Achievements

The goal of this study was to consider whether formalin-fixed human tissues for pathological examination were useful as substitutes for conventional specimens in toxicological analysis. As a first step, we developed UPLC-MS/MS analytical methods to simultaneous analysis for 60 drugs. Some psychotropic drugs were markedly depleted to varying degrees in formalin-fixed porcine liver homogenates. In forensic practice case, the drug was detected in formalin-fixed tissues, only if the concentration of the drug in blood was significantly high. It was suggested that in forensic practice formalin-fixed organs was partially useful for toxicological examination.

Research Products

• [Presentation] The stability of drugs in formalin fixed porcine liver homogenates. (2018)
  • Author(s): Migiwa Asano, Naoki Yoshioka, Azumi Kuse, Natsumi Kuwahara, Yuki Nakabayashi, Motonori Takahashi, Takeshi Kondo, Mai Morichika, Kanako Nakagawa, Makoto Sakurada, Yasuhiro Ueno
  • Organizer: 24th Congress of the International Academy of Legal Medicine
  • Int'l Joint Research
• [Presentation] 血清および臓器中のホルムアルデヒド定量分析法の検討 (2017)
  • Author(s): 浅野水辺、吉岡直樹、久世亜澄、桑原奈都美、高橋玄倫、近藤武史、中川加奈子、森近舞、櫻田誠、上野易弘
  • Organizer: 第101次日本法医学会学術全国集会
  • Place of Presentation: 長良川国際会議場（岐阜県・岐阜市）
  • Year and Date: 2017-06-09
• [Presentation] エンバーミング処置された遺体の血液及び臓器中薬物濃度から薬物中毒死と診断した一例 (2016)
  • Author(s): 浅野水辺、久世亜澄、谷口絵美、桑原奈都美、高橋玄倫、近藤武史、中川加奈子、森近舞、櫻田誠、上野易弘
  • Organizer: 第100次日本法医学会学術全国集会
  • Place of Presentation: きゅりあん（東京都・品川区）
  • Year and Date: 2016-06-16