| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The molecular biological analysis of Japanese aconites was carried out. Diploid species (2n=2x=16) were roughly classified into four geographical groups: Hokkaido, Tohoku, northern Kanto and the southern part of Japan. We could find no species-specific sequence that could identify the species in the tested tetraploid species (2n=4x=32). Since the geographical district-specific sequence was present in the ITS region of the nuclear DNA, the DNA analysis of aconites seems to be useful for the rough estimation of their habitat location.
The composition and concentrations of the four major alkaloids contained in the aconite tuberous roots did not correlate with either the aconite species or the data obtained from a general soil analysis in the examined habitats. However, it was hypothesized that the constitution of each toxic alkaloid or non-toxicity in individual aconites was genetically controlled, because these features were mostly unchanged before and after transplantation.
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