Metabolic profiling analysis of rat administered natural toxins

• Project/Area Number: 15K08889
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Aichi Medical University
• Principal Investigator: OGAWA Tadashi 愛知医科大学, 医学部, 講師 (50559937)
• Co-Investigator(Kenkyū-buntansha): 財津 桂 名古屋大学, 医学系研究科, 准教授 (30700546) ; 石井 晃 名古屋大学, 医学系研究科, 教授 (30252175) ; 妹尾 洋 愛知医科大学, 医学部, 教授 (50236113)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 自然毒 / GC-MS/MS / LC-QTOF-MS / 代謝物

Outline of Final Research Achievements

For the purpose of clarifying influences, toxicities and action mechanisms given by administration of natural toxins in the living body, internal metabolism profiling analyses were worked on rat. Even though establishment of connected evaluation method of metabolomics analysis and iTRAQ protein expression analysis is uncompleted, various fundamental data of metabolic profiling analyses of rat administered natural toxins were obtained by GC-MS/MS and LC-QTOF-MS.

Research Products

• [Journal Article] In vivo metabolic profiling for acetamiprid in rat plasma by liquid chromatography quadrupole time-of-flight mass spectrometry (2018)
  • Author(s): Tadashi Ogawa, Jun Ueyama, Takayoshi Suzuki, Masae Iwai, Xiao-Pen Lee, Takeshi Kumazawa, Keizo Sato, Fumio Kondo, Hiroshi Seno
  • Journal Title: Medical Mass Spectrometry Volume: 2
  • NAID: 130007897981
  • Peer Reviewed / Open Access
• [Journal Article] Sensitive determination of picrotoxin by liquid chromatography-quadrupole time-of-flight mass spectrometry (2016)
  • Author(s): Tadashi Ogawa, Miho Tada, Hideki Hattori, Yosuke Shiraishi, Takayoshi Suzuki, Masae Iwai, Maiko Kusano, Kei Zaitsu, Akira Ishii, Hiroshi Seno
  • Journal Title: Legal Medicine Volume: 20 Pages: 8-11
  • DOI: 10.1016/j.legalmed.2016.12.008
  • Peer Reviewed
• [Journal Article] Inhibition by fructose 1,6-bisphosphate of transaldolase from Escherichia coli (2016)
  • Author(s): Tadashi Ogawa, Keiko Murakami, Masataka Yoshino
  • Journal Title: FEMS Microbiology Letters Volume: 363 Issue: 17 Pages: fnw183-fnw183
  • DOI: 10.1093/femsle/fnw183
  • Peer Reviewed
• [Journal Article] Sensitive determination of picrotoxin by liquid chromatography-quadrupole time-of-flight mass spectrometry (2016)
  • Author(s): Tadashi Ogawa, Miho Tada, Hideki Hattori, Yosuke Shiraishi, Takayoshi Suzuki, Masae Iwai, Maiko Kusano, Kei Zaitsu, Akira Ishii, Hiroshi Seno
  • Journal Title: Legal Medicine Volume: 20 Pages: 8-11
  • DOI: 10.1016/j.legalmed.2016.03.002
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] UPLC-MS/MSを用いた血漿中batrachotoxin およびepibatidine濃度の同時定量 (2017)
  • Author(s): 小川匡之, 白石洋輔, 鈴木隆佳, 岩井雅枝, 近藤文雄, 草野麻衣子, 財津桂, 石井晃, 妹尾洋
  • Organizer: 第2回日本医用マススペクトル学会西部会
• [Presentation] LC-QTOF-MSによるラット血中アセタミプリド代謝物の分析 (2017)
  • Author(s): 小川匡之, 上山純, 鈴木隆佳, 岩井雅枝, 李暁鵬, 熊澤武志, 佐藤啓造, 近藤文雄, 妹尾洋
  • Organizer: 第42回日本医用マススペクトル学会年会
• [Presentation] UPLC-MS-MSを用いたbatrachotoxin, bufotenine, epibatidine血漿中濃度の同時定量 (2016)
  • Author(s): 白石洋輔，小川匡之，鈴木隆佳，岩井雅枝，草野麻衣子，財津桂，石井晃，妹尾洋
  • Organizer: 日本法中毒学会第35年会
  • Place of Presentation: 大阪
  • Year and Date: 2016-07-01
• [Presentation] UPLC-MS-MSを用いたbatrachotoxin, bufotenine, epibatidine血漿中濃度の同時定量 (2016)
  • Author(s): 白石洋輔，小川匡之，鈴木隆佳，岩井雅枝，草野麻衣子，財津桂，石井晃，妹尾洋
  • Organizer: 日本法中毒学会第35 年会
  • Place of Presentation: 大阪
  • Year and Date: 2016-07-01
• [Presentation] Development of multi-targeted screening method for 56 natural toxins in plasma by LC/Q-TOFMS (2015)
  • Author(s): Ogawa T, Zaitsu K, Shiraishi Y, Kusano M, Iwai M, Tsuchihashi H, Ishii A, Seno H
  • Organizer: The 53rd Annual Meeting of the International Association of Forensic Toxicologists
  • Place of Presentation: Firenze, Italy
  • Year and Date: 2015-08-30
  • Int'l Joint Research
• [Presentation] 血漿中自然毒一斉抽出法の検討 (2015)
  • Author(s): 小川匡之，財津桂，白石洋輔，草野麻衣子，岩井雅枝，土橋均，石井晃，妹尾洋
  • Organizer: 日本法中毒学会第34 年会
  • Place of Presentation: 福岡
  • Year and Date: 2015-06-26