Determination of drug intake day by micro-segmental hair analysis

• Project/Area Number: 15K08894
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: National Research Institute of Police Science
• Principal Investigator: Kuwayama Kenji 科学警察研究所, 法科学第三部, 主任研究官 (40356233)
• Co-Investigator(Kenkyū-buntansha): 宮口 一 科学警察研究所, 法科学第三部, 室長 (10370884)
• Project Period (FY): 2015-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 毛髪 / 薬物 / 質量分析

Outline of Final Research Achievements

In this study, a method to detect drugs in 0.4-mm hair segments, corresponding to the hair growth length of a day, using liquid chromatography/tandem mass spectrometry (micro-segmental analysis) was developed. Moreover, the day of drug intake was determined using micro-segmental hair analysis based on ingestion of internal temporal markers before hair collection.

Academic Significance and Societal Importance of the Research Achievements

従来の毛髪中薬物の分画分析では、十分な検出感度を得るために数十本の毛髪を束ねた上で数cm間隔に分画するので、数か月単位での薬物摂取時期の推定しかできなかった。単毛髪のマイクロ分画分析の開発により、被疑者の供述、被害者の記憶等によらず科学的に日数単位での薬歴を把握でき、毛髪中薬物鑑定の証明力を向上させることができる。

Research Products

• [Journal Article] Measurement of three-dimensional distributions of drugs in nails using liquid chromatography/tandem mass spectrometry after micro-segmentation to elucidate drug uptake routes (2020)
  • Author(s): Kuwayama Kenji、Miyaguchi Hajime、Kanamori Tatsuyuki、Tsujikawa Kenji、Yamamuro Tadashi、Segawa Hiroki、Okada Yuki、Iwata Yuko T.
  • Journal Title: Analytica Chimica Acta Volume: 1108 Pages: 89-97
  • DOI: 10.1016/j.aca.2020.02.050
  • Peer Reviewed
• [Journal Article] Development of High-resolution Methods for the Analysis of Drug Distribution in Biological Tissue Samples and Their Applications (2019)
  • Author(s): Kuwayama Kenji
  • Journal Title: YAKUGAKU ZASSHI Volume: 139 Issue: 8 Pages: 1063-1070
  • DOI: 10.1248/yakushi.19-00112
  • NAID: 130007686243
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2019-08-01
  • Peer Reviewed / Open Access
• [Journal Article] Strong evidence of drug-facilitated crimes by hair analysis using LC-MS/MS after micro-segmentation (2019)
  • Author(s): Kuwayama Kenji、Miyaguchi Hajime、Iwata Yuko T.、Kanamori Tatsuyuki、Tsujikawa Kenji、Yamamuro Tadashi、Segawa Hiroki、Inoue Hiroyuki
  • Journal Title: Forensic Toxicology Volume: 37 Issue: 2 Pages: 480-487
  • DOI: 10.1007/s11419-019-00472-3
  • Peer Reviewed
• [Journal Article] Micro-segmental hair analysis for proving drug-facilitated crimes: Evidence that a victim ingested a sleeping aid, diphenhydramine, on a specific day (2018)
  • Author(s): Kuwayama Kenji、Nariai Maika、Miyaguchi Hajime、Iwata Yuko T.、Kanamori Tatsuyuki、Tsujikawa Kenji、Yamamuro Tadashi、Segawa Hiroki、Abe Hiroko、Iwase Hirotaro、Inoue Hiroyuki
  • Journal Title: Forensic Science International Volume: 288 Pages: 23-28
  • DOI: 10.1016/j.forsciint.2018.04.027
  • Peer Reviewed
• [Journal Article] Estimation of day of death using micro-segmental hair analysis based on drug use history: a case of lidocaine use as a marker (2018)
  • Author(s): Kuwayama Kenji、Nariai Maika、Miyaguchi Hajime、Iwata Yuko T.、Kanamori Tatsuyuki、Tsujikawa Kenji、Yamamuro Tadashi、Segawa Hiroki、Abe Hiroko、Iwase Hirotaro、Inoue Hiroyuki
  • Journal Title: International Journal of Legal Medicine Volume: 133 Issue: 1 Pages: 117-122
  • DOI: 10.1007/s00414-018-1939-9
  • Peer Reviewed
• [Journal Article] Accurate Estimation of Drug Intake Day by Microsegmental Analysis of a Strand of Hair by Use of Internal Temporal Markers (2018)
  • Author(s): Kuwayama Kenji、Nariai Maika、Miyaguchi Hajime、Iwata Yuko T.、Kanamori Tatsuyuki、Tsujikawa Kenji、Yamamuro Tadashi、Segawa Hiroki、Abe Hiroko、Iwase Hirotaro、Inoue Hiroyuki
  • Journal Title: The Journal of Applied Laboratory Medicine Volume: 3 Issue: 1 Pages: 37-47
  • DOI: 10.1373/jalm.2017.025346
  • Peer Reviewed
• [Journal Article] Different localizations of drugs simultaneously administered in a strand of hair by micro-segmental analysis (2017)
  • Author(s): Kuwayama Kenji、Miyaguchi Hajime、Iwata Yuko T.、Kanamori Tatsuyuki、Tsujikawa Kenji、Yamamuro Tadashi、Segawa Hiroki、Inoue Hiroyuki
  • Journal Title: Drug Testing and Analysis Volume: 10 Issue: 4 Pages: 750-760
  • DOI: 10.1002/dta.2259
  • Peer Reviewed
• [Journal Article] Enantioselective determination of ( R )-zopiclone and ( S )-zopiclone (eszopiclone) in human hair by micropulverized extraction and chiral liquid chromatography/high resolution mass spectrometry (2017)
  • Author(s): Miyaguchi Hajime、Kuwayama Kenji
  • Journal Title: Journal of Chromatography A Volume: 1519 Pages: 55-63
  • DOI: 10.1016/j.chroma.2017.08.058
  • Peer Reviewed
• [Journal Article] Time-course measurements of drug concentrations in hair and toenails after single administrations of pharmaceutical products (2016)
  • Author(s): Kenji Kuwayama, Hajime Miyaguchi, Yuko T. Iwata, Tatsuyuki Kanamori, Kenji Tsujikawa, Tadashi Yamamuro, Hiroki Segawa, Hiroyuki Inoue
  • Journal Title: Drug Testing and Analysis Volume: 印刷中 Issue: 4 Pages: 571-577
  • DOI: 10.1002/dta.1991
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Three-step drug extraction from a single sub-millimeter segment of hair and nail to determine the exact day of drug intake (2016)
  • Author(s): Kenji Kuwayama, Hajime Miyaguchi, Yuko T. Iwata, Tatsuyuki Kanamori, Kenji Tsujikawa, Tadashi Yamamuro, Hiroki Segawa, Hiroyuki Inoue
  • Journal Title: Analytica Chimica Acta Volume: 948 Pages: 40-47
  • DOI: 10.1016/j.aca.2016.10.029
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Highly sensitive quantification of unconjugated 11-nor-9-carboxy-Δ9-tetrahydrocannabinol in a cannabis user’s hair using micropulverized extraction (2016)
  • Author(s): Kenji Kuwayama, Hajime Miyaguchi, Tadashi Yamamuro, Kenji Tsujikawa, Tatsuyuki Kanamori, Yuko T. Iwata, Hiroyuki Inoue
  • Journal Title: Forensic Science International Volume: 262 Pages: e34-e36
  • DOI: 10.1016/j.forsciint.2016.03.009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Micro-pulverized extraction pretreatment for highly sensitive analysis of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol in hair by liquid chromatography/tandem mass spectrometry (2015)
  • Author(s): Kenji Kuwayama, Hajime Miyaguchi, Tadashi Yamamuro, Kenji Tsujikawa, Tatsuyuki Kanamori, Yuko T. Iwata, Hiroyuki Inoue
  • Journal Title: Rapid Communications in Mass Spectrometry Volume: 29 Issue: 22 Pages: 2158-2166
  • DOI: 10.1002/rcm.7363
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] マイクロ分画分析を用いた毛髪及び爪内薬物分布に基づく薬物取込み経路の解析 (2020)
  • Author(s): 桑山健次，宮口一，金森達之，辻川健治，山室匡史，瀬川尋貴，岩田祐子
  • Organizer: 日本薬学会第140年会
• [Presentation] 毛髪を用いた睡眠薬悪用犯罪の証明：高分解能質量分析による薬物スクリーニングとマイクロ分画分析による薬物摂取日の特定 (2019)
  • Author(s): 桑山健次，宮口一，岩田祐子，金森達之，辻川健治，山室匡史，瀬川尋貴，井上博之
  • Organizer: 日本法中毒学会第38年会
• [Presentation] 法科学における毛髪中薬物分析の有用性 (2019)
  • Author(s): 桑山健次
  • Organizer: 第46回日本毒性学会学術年会
  • Invited
• [Presentation] 毛髪及び爪を用いた薬物摂取日特定法の開発 －毛髪及び爪の成長速度等のデータ収集－ (2019)
  • Author(s): 桑山健次，宮口一，岩田祐子，金森達之，辻川健治，山室匡史，瀬川尋貴，井上博之
  • Organizer: 日本薬学会第139年会
• [Presentation] 単毛髪のマイクロ分画分析を利用した死亡日推定 (2018)
  • Author(s): 桑山健次，成相舞花，宮口一，岩田祐子，金森達之，辻川健治，山室匡史，瀬川尋貴，安部寛子，岩瀬博太郎，井上博之
  • Organizer: 日本法中毒学会第37年会
• [Presentation] 微小分画分析による体組織内の薬物の高解像度分布測定法の開発と応用 (2018)
  • Author(s): 桑山健次
  • Organizer: 第62回日本薬学会関東支部大会
  • Invited
• [Presentation] 睡眠薬悪用犯罪の証明力強化 －毛髪のマイクロ分画分析による薬物摂取日の特定－ (2018)
  • Author(s): 桑山健次，宮口一，岩田祐子，金森達之，辻川健治，山室匡史，瀬川尋貴，井上博之
  • Organizer: 日本法科学技術学会第24回学術集会
• [Presentation] 指標物質を用いた単毛髪のマイクロ分画分析による薬物摂取日推定法の実用化に向けた評価 (2018)
  • Author(s): 桑山健次，成相舞花，宮口一，岩田祐子，金森達之，辻川健治，山室匡史，瀬川尋貴，安部寛子，岩瀬博太郎，井上博之
  • Organizer: 日本薬学会第138年会
• [Presentation] 微小分画分析による毛髪及び爪内の薬物分布測定 (2017)
  • Author(s): 桑山健次、宮口一、岩田祐子、金森達之、辻川健治、山室匡史、瀬川尋貴、井上博之
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台市
  • Year and Date: 2017-03-25
• [Presentation] マイクロ分画分析による同時摂取した薬物間の毛髪内分布の違い (2017)
  • Author(s): 桑山健次，成相舞花，宮口一，岩田祐子，金森達之，辻川健治，山室匡史，瀬川尋貴，安部寛子，岩瀬博太郎，井上博之
  • Organizer: 日本法中毒学会第36年会
• [Presentation] ワンチューブマイクロ粉砕抽出法とキラル液体クロマトグラフィー/高分解能質量分析による毛髪中ゾピクロンの定量分析 (2017)
  • Author(s): 宮口一，桑山健次
  • Organizer: 日本法中毒学会第36年会
• [Presentation] 単毛髪のマイクロ分画分析による薬物摂取日の高精度推定法の開発 (2017)
  • Author(s): 桑山健次，成相舞花，宮口一，岩田祐子，金森達之，辻川健治，山室匡史，瀬川尋貴，安部寛子，岩瀬博太郎，井上博之
  • Organizer: 日本法科学技術学会第23回学術集会
• [Presentation] 毛髪中の非抱合型THC-COOH及び総THC-COOHの定量分析 (2016)
  • Author(s): 桑山健次、阿久沢尚士、宮口 一、岩田祐子、金森達之、辻川健治、山室匡史、瀬川尋貴、井上博之
  • Organizer: 日本法科学技術学会第22回学術集会
  • Place of Presentation: 東京都中野区
  • Year and Date: 2016-11-10
• [Presentation] Time-course measurements of drug concentrations in hair and nails after single administrations of pharmaceutical products by LC/MS/MS (2016)
  • Author(s): Kenji Kuwayama, Hajime Miyaguchi, Yuko T. Iwata, Tatsuyuki Kanamori, Kenji Tsujikawa, Tadashi Yamamuro, Hiroki Segawa, Hiroyuki Inoue
  • Organizer: Asia Pacific Mass Spectrometry 2016
  • Place of Presentation: Kuala lumpur, Malaysia
  • Year and Date: 2016-10-10
  • Int'l Joint Research
• [Presentation] Comparison of extraction methods for highly accumulated zolpidem in Japanese black hair (2016)
  • Author(s): Hajime Miyaguchi, Kenji Kuwayama
  • Organizer: TIAFT meeting 2016
  • Place of Presentation: Brisbane, Australia
  • Year and Date: 2016-08-28
  • Int'l Joint Research
• [Presentation] 毛髪中の薬物分析 (2016)
  • Author(s): 宮口 一
  • Organizer: 第77回分析化学討論会
  • Place of Presentation: 岐阜市
  • Year and Date: 2016-05-27
  • Invited
• [Presentation] 単回摂取された薬物の毛髪及び爪中濃度の経時変化 (2016)
  • Author(s): 桑山 健次、瀬川 尋貴、山室 匡史、辻川 健治、金森 達之、岩田 祐子、井上 博之
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 横浜市
  • Year and Date: 2016-03-27
• [Presentation] Comparison between urine, blood, saliva, and fingerprints in forensic drug testing (2015)
  • Author(s): Kenji Kuwayama, Hajime Miyaguchi, Tadashi Yamamuro, Kenji Tsujikawa, Tatsuyuki Kanamori, Yuko T. Iwata, Hiroyuki Inoue
  • Organizer: TIAFT meeting 2015
  • Place of Presentation: Florence, Italy
  • Year and Date: 2015-08-30
  • Int'l Joint Research
• [Presentation] 尿や血液の代替資料からの薬物検査 ―唾液、皮膚分泌物、毛髪、爪― (2015)
  • Author(s): 桑山 健次、山室 匡史、辻川 健治、宮口 一、金森 達之、岩田 祐子、井上 博之
  • Organizer: 日本法中毒学会第34年会
  • Place of Presentation: 福岡市
  • Year and Date: 2015-06-26
• [Presentation] 毛髪中ゾルピデム分析における２つの落とし穴：蓄積挙動の人種差と前処理法の抽出能力について (2015)
  • Author(s): 宮口 一、桑山 健次
  • Organizer: 日本法中毒学会第34年会
  • Place of Presentation: 福岡市
  • Year and Date: 2015-06-26