The mechanism of metastasis and metastasis suppression in gastric carcinoma by molecular interactions between intracellular target molecules and metastasis suppressor protein Nm23-H1

• Project/Area Number: 15K08951
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kochi Gakuen College (2016-2017); Kochi University (2015)
• Principal Investigator: MURAKAMI MASANAO 高知学園短期大学, その他部局等, 准教授(移行) (80571017)
• Co-Investigator(Kenkyū-buntansha): 大畑 雅典 高知大学, 教育研究部医療学系基礎医学部門, 教授 (50263976)
• Research Collaborator: Derek Kennedy グリフィス大学, エスキティス研究所, 准教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 胃がん / がん転移抑制 / Nm23-H1 / Asef / G3BP / G3BP1 / G3BP2 / 癌転移抑制蛋白質 / 低分子Gタンパク質 / スキルス胃癌 / Asef2 / Cdc42 / cell migration

Outline of Final Research Achievements

We had identified several molecules as a target protein of metastasis suppressor protein Nm23-H1. To clear the mechanism of how cancer cells were obtained ability of migration, we investigated the relationship between Nm23-H1 and candidate of binding proteins which we identified previously. ①Asef2 was identified as a target protein of Nm23-H1, which was stimulated by adenomatous polyposis coli. The GEF activity of Asef was enhanced in scirrhous type gastric carcinoma cell lines and then led to high migration activities, however exogenous Nm23-H1 again suppressed GEF activity. ②G3BP1, 2a and 2b (G3BPs) that are associated with breast cancer and pancreatic cancer, were also bound to Nm23-H1, but there was a difference in the binding strength. Each G3BP molecules have different functions. G3BP1 is known to associate with stress granules formation. Co-transfected cells of G3BPs and Nm23-H1 showed co-localization in the cytoplasm as punctate signals and in all cases.

Research Products

• [Int'l Joint Research] Department of Microbiology/Perelman School of Medicine/University of Pennsylvania(米国)
• [Journal Article] Ecology of Merkel cell Polyomavirus in Healthy Skin among individuals in an Asian Cohort. (2016)
  • Author(s): 1.Yumiko Hashida, Mikio Kamioka, Moe Tanaka, Sena Hosokawa, Masanao Murakami, Kimiko Nakajima, Hiroaki Kikuchi, Mikiya Fujieda, Shigetoshi Sano, Masanori Daibata.
  • Journal Title: Journal of Infectious Diseases. Volume: 213 Pages: 1708-1716
  • Peer Reviewed / Open Access
• [Journal Article] High load of Merkel cell polyomavirus DNA detected in the normal skin of Japanese patients with Merkel cell carcinoma. (2016)
  • Author(s): 2.Yumiko Hashida, Kimiko Nakajima, Hideki Nakajima, Takeo Shiga, Moe Tanaka, Masanao Murakami, Shigenobu Matsuzaki, Seiji Naganuma, Naoki Kuroda, Yasutaka Seki, Harutaka Katano, Shigetoshi Sano, Masanori Daibata.
  • Journal Title: J. Clin. Virol. Volume: 82 Pages: 101-107
  • Peer Reviewed
• [Journal Article] Ecology of Merkel cell polyomavirus in healthy skin among individual in an Asian cohort (2016)
  • Author(s): Hashida Y, Kamioka M, Tanaka M, Hosokawa S, Murakami M, Nakajima K, Kikuchi H, Fujieda M, Sano S, Daibata M.
  • Journal Title: J. infect. Dis Volume: 213(11) Pages: 1708-1716
  • Peer Reviewed
• [Journal Article] Prevalence of human cytomegalovirus, polyomaviruses, and oncogenic viruses in glioblastoma among Japanese subjects. (2015)
  • Author(s): Yumiko Hashida, Ayuko Taniguchi, Toshio Yawata, Sena Hosokawa, Masanao Murakami, Makoto Hiroi, Tetsuya Ueba, Masanori Daibata.
  • Journal Title: Infectious Agents and Cancer. Volume: 10 Issue: 1 Pages: 3-3
  • DOI: 10.1186/1750-9378-10-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Epidemiological study of Merkel cell polyomavirus in healthy skin among healthy individuals in Japan. (2016)
  • Author(s): 1.Hashida Y, Tanaka M, Murakami M, Matsuzaki S, Daibata M.
  • Organizer: 第64回日本ウイルス学会学術集会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-10-23
• [Presentation] Phylogenetic analysis of Merkel cell polyomavirus based on full-length LT and VP1 sequences derived from normal skin of healthy individuals. (2016)
  • Author(s): 2.Tanaka M, Hashida Y, Murakami M, Matsuzaki S, Daibata M.
  • Organizer: 第64回日本ウイルス学会学術集会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-10-23
• [Presentation] Virus infection in glioblastoma multiform (GBM): Possible association of human papillomavirus with pathogenesis of GBM. (2015)
  • Author(s): Hashida Y, Taniguchi A, Yawata T, Hoshikawa S, Tanaka M, Murakami M, Kamioka M, Hiroi M, Ueba T, and Daibata M
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場・（福岡県）
  • Year and Date: 2015-11-22
• [Presentation] Nm23-H1 regulates cell migration via interaction with Asef1 and Asef2. (2015)
  • Author(s): Murakami M, Hashida Y, Kamioka M, Kawasaki Y, Yanagihara K, Matsuzasi S, Akiyama Tetsu, Daibata M.
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場・（愛知県）
  • Year and Date: 2015-10-08
• [Presentation] Mycobaterium smegmatis バクテリオファージの長期保存状態における生存率 (2015)
  • Author(s): Mstuzaki S, Ohhara N, Taniguchi H, Yamasaki T, Ujihata T, Murakami M, Daibata M.
  • Organizer: 第68回日本細菌学会中国・四国支部総会
  • Place of Presentation: 岡山大学・（岡山県）
  • Year and Date: 2015-10-03
• [Book] 臨床と微生物 (2015)
  • Author(s): 松崎茂展、氏原隆子、村上雅尚、大畑雅典
  • Total Pages: 740
  • Publisher: 近代出版