| Project/Area Number |
15K08956
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
Handa Osamu 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90381970)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
内藤 裕二 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00305575)
水島 かつら 京都府立医科大学, 医学(系)研究科(研究院), 助教 (20564115)
木下 健司 武庫川女子大学, 薬学部, 教授 (70441219)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 唾液 / ヘリコバクター・ピロリ / PCR / 感染診断 / ヘリコバクター・ピロリ菌 / 安価 / 迅速 / 抗生剤感受性 / 感受性試験 / 非侵襲 / Helicobacter pylori |
|---|
| Outline of Final Research Achievements |
Diagnosis for H. pylori requires time and expenses. In this study, we focused on "the existence of H. pylori in saliva" as previously reported and by using saliva, attempted to develop a rapid and inexpensive H. pylori infection diagnosis and an antibiotic susceptibility test. First, using a standard solution in which H. pylori was reliably present, PCR was carried out with a template (primer) of H. pylori DNA as previously reported and an H. pylori-specific peak was recognized. However, in patients saliva (infected / non-infected), no specific peak was observed. We tried modification of PCR condition, use of other primers, concentration of specimen, change to primer for toxin DNA of H. pylori, but H. pylori in saliva was not detectable.
|
