Galectin-3 Promotes Cell Proliferation and Attenuates Cytotoxic Effect of Paclitaxel via ERK Signaling Pathway in Gastric Cancer Cells.
| Project/Area Number |
15K08959
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
永原 章仁 順天堂大学, 医学部, 教授 (00266040)
北條 麻理子 順天堂大学, 医学部, 准教授 (60372934)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ガレクチン-3 / 胃癌 / パクリタキセル / ERKシグナル伝達系 |
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| Outline of Final Research Achievements |
Our aim in this study is to assess whether silencing of galectin-3 contributes to the inhibition of gastric cancer cell proliferation and enhances the effect of paclitaxel through ERK (Extracellular Signal-regulated Kinase) signaling pathway.
The proliferating cells were decreased in galectin-3 siRNA transfected cells compared to control cells. Western blot showed that phosphorylation of ERK was reduced in galectin-3 siRNA transfected cells. Cell proliferation in galectin-3 siRNA transfected cells was decreased compared to control cells in the presence of paclitaxel (TXL). Western blot showed that phosphorylation of ERK was reduced in galectin-3 siRNA/TXL combination compared to scrambled siRNA/TXL combination. These results indicate that silencing of galectin-3 downregulates ERK signaling pathway and enhances the effect of TXL, leading to synergistic inhibition of cell proliferation in gastric cancer.
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Report
(4 results)
Research Products
(3 results)
