Therapeutic drug monitoring for IBD

• Project/Area Number: 15K08967
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Andoh Akira 滋賀医科大学, 医学部, 教授 (90252395)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: IBD / 抗TNF抗体 / 炎症性腸疾患 / 抗サイトカイン療法 / IgG / アダリムマブ

Outline of Final Research Achievements

he present report describes a new ELISA system for measurement of serum ADA concentrations. In this system, non-specific binding of serum IgG was markedly blocked and did not require specific materials, such as anti-ADA idiotype antibodies. In addition, this study describes a novel immunoassay for AAAs that can measure AAAs in the presence of free ADA. These methods improved the diagnostic accuracy for measurement of serum ADA and AAA concentrations and might be useful for determination of the therapeutic approach for patients with a loss of response to ADA.

Research Products

• [Journal Article] Successful treatment by fecal microbiota transplantation for Japanese patients with refractory Clostridium difficile infection: A prospective case series. (2017)
  • Author(s): Bamba S, Nishida A, Imaeda H, Inatomi O, Sasaki M, Sugimoto M, Andoh A.
  • Journal Title: J Microbiol Immunol Infect. Volume: 201717 Issue: 4 Pages: 30235-9
  • DOI: 10.1016/j.jmii.2017.08.027
  • Peer Reviewed / Open Access
• [Journal Article] Enterococcus durans TN-3 Induces Regulatory T Cells and Suppresses the Development of Dextran Sulfate Sodium (DSS)-Induced Experimental Colitis (2016)
  • Author(s): Kanda T, Nishida A, Ohno T, Imaeda H, Takahashi K, Inatomi O, Bamba S, Sugimoto M, Andoh A
  • Journal Title: Pros One Volume: 11 Issue: 7 Pages: e0159705-e0159705
  • DOI: 10.1371/journal.pone.0159705
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of the gut microbial community between obese and lean peoples using 16S gene sequencing in a Japanese population (2016)
  • Author(s): Andoh A, Nishida A, Takahashi K, Inatomi O, Imaeda H, Bamba S, Kito K, Sugimoto M, Kobayashi T
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 59 Issue: 1 Pages: 65-70
  • DOI: 10.3164/jcbn.15-152
  • NAID: 130005160848
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy and safety of single fecal microbiota transplantation for Japanese patients with mild to moderately active ulcerative colitis. (2016)
  • Author(s): Nishida A1, Imaeda H1, Ohno M1, Inatomi O1, Bamba S1, Sugimoto M1, Andoh A2
  • Journal Title: J Gastroenterol. Volume: 52 Issue: 4 Pages: 476-482
  • DOI: 10.1007/s00535-016-1271-4
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Increased Expression of Interleukin-36, a Member of the Interleukin-1 Cytokine Family, in Inflammatory Bowel Disease. (2016)
  • Author(s): Nishida A, Hidaka K, Kanda T, Imaeda H, Shioya M, Inatomi O, Bamba S, Kitoh K, Sugimoto M, Andoh A.
  • Journal Title: Inflammatory bowel disease Volume: 22 Issue: 2 Pages: 303-314
  • DOI: 10.1097/mib.0000000000000654
  • Peer Reviewed / Open Access / Acknowledgement Compliant