Role of galectin-3 and intestinal bacteria flora on NSAID-induced small intestinal ulcer

• Project/Area Number: 15K08975
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kindai University
• Principal Investigator: PARK Ah-Mee 近畿大学, 医学部, 講師 (70469245)
• Co-Investigator(Kenkyū-buntansha): 萩原 智 近畿大学, 医学部, 講師 (40460852)
• Research Collaborator: TSUNODA Ikuo 近畿大学, 医学部, 教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ガレクチン-3 / 腸内フローラ / NSAIDs / 小腸潰瘍 / ガレクチン－３ / 小腸フローラ / マクロファージ / ガレクチン-３ / ケモカインレセプター9 / インドメタシン / ガレクチンｰ3 / 小腸 / 炎症

Outline of Final Research Achievements

The purpose of this study is to clarify the role of Galectin-3 (Gal3) on NSAIDs-induced intestinal ulcer. By using Gal3 knockout mice, we found that Gal-3 is a worsen factor of NSAIDs-induced intestinal ulcer. There are two possible mechanism for this negative effect of Gal3. The first one is a role of Gal3 as danger signal of activated macrophages. This molecule might cause sever inflammation. The another one is changes of intestinal bacterial flora with or without Gal-3. We found obvious differences of small intestinal flora population between wildtype mice and Gal3 mice. Those differences might affect to the ulceration by NSAIDs.

Research Products

• [Journal Article] Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: novel implication for vascular atrophy (2018)
  • Author(s): Kishimoto A, Kimura S, Nio-Kobayashi J, Takahashi-Iwanaga H, Park AM, Iwanaga T
  • Journal Title: Exp Eye Res Volume: 未定 Pages: 1-9
  • DOI: 10.1016/j.exer.2018.03.024
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Murine γ-Herpesvirus 68 Induces Severe Lung Inflammation in IL-27-Deficient Mice with Liver Dysfunction Preventable by Oral Neomycin. (2018)
  • Author(s): Kyosuke Kanai, Ah-Mee Park, Akiko Watanabe, Tomohiro Arikawa, Teruhito Yasui, Hiroki Yoshida, Ikuo Tsunoda and Osamu Yoshie.
  • Journal Title: J Immunol Volume: 200 Issue: 8 Pages: 2703-2713
  • DOI: 10.4049/jimmunol.1700412
  • Peer Reviewed
• [Journal Article] Helicobacter pylori and gut microbiota in multiple sclerosis versus Alzheimer's disease: 10 pitfalls of microbiome studies (2017)
  • Author(s): Park Ah-Mee、Omura Seiichi、Fujita Mitsugu、Sato Fumitaka、Tsunoda Ikuo
  • Journal Title: Clin Exp Neuroimmunol Volume: 8 Issue: 3 Pages: 215-232
  • DOI: 10.1111/cen3.12401
  • Peer Reviewed / Open Access
• [Journal Article] IL-35 suppresses lipopolysaccharide-induced airway eosinophilia in EBI3-deficient mice. (2017)
  • Author(s): Kanai K, Park AM, Yoshida H, Tsunoda I, Yoshie O.
  • Journal Title: Journal of Immunology Volume: 198(1) Issue: 1 Pages: 119-127
  • DOI: 10.4049/jimmunol.1600506
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Galectin-3 Plays an Important Role in Innate Immunity to Gastric Infection by Helicobacter pylori. (2016)
  • Author(s): Park AM, Hagiwara S, Hsu DK, Liu FT, Yoshie O.
  • Journal Title: Infection and Immunity Volume: 84(4) Issue: 4 Pages: 1184-1193
  • DOI: 10.1128/iai.01299-15
  • Peer Reviewed
• [Journal Article] Heat Shock Protein 27 Plays a Pivotal Role in Myofibroblast Differentiation and in the Development of Bleomycin-Induced Pulmonary Fibrosis (2016)
  • Author(s): Park AM, Kanai K, Itoh T, Sato T, Tsukui T, Inagaki Y, Selman M, Matsushima K, Yoshie O.
  • Journal Title: Plos One Volume: 11 Issue: 2 Pages: e0148998-e0148998
  • DOI: 10.1371/journal.pone.0148998
  • Peer Reviewed / Open Access
• [Journal Article] mpaired expression of ATP-binding cassette transporter G2 and liver damage in erythropoietic protoporphyria (2015)
  • Author(s): Hagiwara S, Nishida N, Park AM, Sakurai T, Kawada A
  • Journal Title: Hepatology Volume: 62 Issue: 5 Pages: 1638-1639
  • DOI: 10.1002/hep.27871
  • Peer Reviewed / Open Access
• [Presentation] Novel function of p130 protein in pulmonary fibrosis (2017)
  • Author(s): Ah-Mee Park, Ikuo Tsunoda
  • Organizer: ConBio 2017
• [Presentation] HSP27ノックダウンによる線維芽細胞の細胞周期停止におけるE2F-4の役割 (2016)
  • Author(s): 朴雅美、角田郁夫
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] ヘリコバクター・ピロリ菌感染おけるガレクチン-3の生体防御的役割 (2016)
  • Author(s): 朴雅美、義江修
  • Organizer: 第81回日本インターフェロン・サイトカイン学会
  • Place of Presentation: 長崎大学（長崎県長崎市）
  • Year and Date: 2016-05-13
• [Presentation] IL-35は気道における好酸球浸潤を抑制する (2016)
  • Author(s): 金井 亨輔、朴 雅美、角田 郁生、吉田 裕樹、義江 修
  • Organizer: 第81回日本インターフェロン・サイトカイン学会
  • Place of Presentation: 長崎大学（長崎県長崎市）
  • Year and Date: 2016-05-13
• [Presentation] Galectin-3 plays important protective roles against H. pylori infection. (2015)
  • Author(s): 朴雅美、義江修
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Galectin-3 is involved in H. pylori killing by macrophages. (2015)
  • Author(s): 朴雅美、義江修
  • Organizer: がん免疫療法・マクロファージ国際会議2015
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-07-09
  • Int'l Joint Research