Analysis of quasispecies of HBV / HCV genes in a single individual by a new sequencing technique
| Project/Area Number |
15K08995
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
SAKAMOTO Minoru 山梨大学, 大学院総合研究部, 准教授 (60324191)
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| Co-Investigator(Kenkyū-buntansha) |
榎本 信幸 山梨大学, 大学院総合研究部, 教授 (20251530)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | HBV / HCV / quasispecies / deep sequencing / B型肝炎ウイルス / C型肝炎ウイルス / 薬剤耐性遺伝子 / Pre S2遺伝子 / DAA / 核酸アナログ治療 / B型肝炎 / C型肝炎 / 薬剤耐性変異 |
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| Outline of Final Research Achievements |
The quasispecies state of HBV, HCV genome was analyzed by new sequencing technology.
In HCV, the drug resistance mutation against DAA is minor clone so called drug resistant associated variants: RAV becomes dominant after treatment. In HBV, quasispecies state in which deletion mutant of HBV-PreS2 region and Pre S1 / S2 initiation codon mutation are existed. It is formed by persistent infection with or without antiviral treatment and existent ratio 1% or more is associated with the disease state. Further In HCC cases, an increase in the existent ratio of mutated clone in PreS2 region is closely related to liver carcinogenesis.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Association Between Alanine Aminotransferase Elevation And UGT1A1*6 Polymorphisms In Daclatasvir And Asunaprevir Combination Therapy For Chronic Hepatitis C.2017
Author(s)
Maekawa S, Sato M, Kuratomi N, Inoue T, Suzuki Y, Tatsumi A, Miura M, Matsuda S, Muraoka M, Nakakuki N, Amemiya F, Takano S, Fukasawa M, Nakayama Y, Yamaguchi T, Sato T, Sakamoto M, Murakawa M, Nakagawa M, Asahina Y, Enomoto N.
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Journal Title
J Gastroenterol
Volume: 印刷中
Issue: 6
Pages: 780-786
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular carcinoma with clinicopathological features.2016
Author(s)
Kawai-Kitahata F, Asahina Y, Tanaka S, Kakinuma S, Murakawa M, Sayuri Nitta S, Watanabe T, Otani S, Taniguchi M, Goto F, Nagata H, Kaneko S, Tasaka-Fujita M, Nishimura-Sakurai Y, Azuma S, Itsui Y, Nakagawa M, Tanabe M, Takano S, Fukasawa M, Sakamoto M, Maekawa S, Enomoto N, Watanabe M.
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Journal Title
Journal of Gastroenterology
Volume: 51
Issue: 5
Pages: 473-486
DOI
Related Report
Peer Reviewed
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[Journal Article] Induction of IFN-lambda3 as an additional effect of nucleotide, not nucleoside, analogues: a new potential target for HBV infection2016
Author(s)
Murata, K. Asano, M. Matsumoto, A. Sugiyama, M. Nishida, N. Tanaka, E. Inoue, T. Sakamoto, M. Enomoto, N. Shirasaki, T. Honda, M. Kaneko, S. Gatanaga, H. Oka, S. Kawamura, Y. I. Dohi, T. Shuno, Y. Yano, H. Mizokami, M.
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Journal Title
Gut
Volume: -
Issue: 2
Pages: 362-371
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Deep sequencing and phylogenetic analysis of variants resistant to interferon-based protease inhibitor therapy in chronic hepatitis induced by genotype 1b hepatitis C virus.2015
Author(s)
Sato M, Maekawa S, Komatsu N, Tatsumi A, Miura M, Muraoka M, Suzuki Y, Amemiya F, Takano S, Fukasawa M, Nakayama Y, Yamaguchi T, Uetake T, Inoue T, Sato T, Sakamoto M, Yamashita A, Moriishi K, Enomoto N.
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Journal Title
J Virol
Volume: 89(11)
Issue: 11
Pages: 6105-16
DOI
Related Report
Peer Reviewed / Open Access
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