| Project/Area Number |
15K09018
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Itoh Yoshito 京都府立医科大学, 医学(系)研究科(研究院), 教授 (70244613)
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| Co-Investigator(Kenkyū-buntansha) |
山口 寛二 京都府立医科大学, 医学(系)研究科(研究院), 助教 (50381950)
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| Co-Investigator(Renkei-kenkyūsha) |
Fujita Jun 京都大学, 医学研究科, 教授 (50173430)
Itoh Katsuhiko 京都大学, 医学研究科, 准教授 (90281097)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Apg-2 / 分子シャペロン / 肝癌 / 細胞周期 / ユビキチン化 / 脂肪肝炎 |
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| Outline of Final Research Achievements |
Heat shock proteins (HSPs) not only protect cells from the toxic effects of heat and other stresses but also have multiple housekeeping functions, such as folding and protein degradation. Apg-2 is a subfamily of Hsp110 and has been reported to be overexpressed in human hepatocellular carcinomas (HCCs). To study the role of Apg-2 in liver steatosis and carcinogenesis, we generated Apg-2 deficient mice and examined high fat diet-induced liver steatosis and diethylnitrosamine (DEN)-induced hepatocellular carcinomas (HCCs). We found that Apg-2 deficient mice exhibited less liver steatosis and DEN-induced hepatocellular carcinomas (HCCs) compared to wild-types. RNA sequence from Apg-2 deficient HCCs and in vitro study of hepatoma cell lines revealed that Apg-2 regulated cell cycle G2/M pathway-related molecules, such as CDK1/CyclinB1, and promoted HCCs proliferations. These findings indicated that Apg-2 is a potential target for the therapy of HCCs.
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