| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In this study, I evaluated the accumulation of HBsAg in hepatocytes by using the HBsAg expression vectors. I prepared the HBsAg expression vectors with several clones of genotypes A, B, and C. These vectors were transfected to HepG2 cells and intra- and extra-cellular HBsAg was measured. The expression of HBsAg of genotype C clones resulted in an intra-cellular accumulation of HBsAg in comparison with other genotypes. I also evaluated the effects of polymorphisms at the promoter region of HBsAg. I prepared the HBsAg expression vector harboring the amino acid polymorphisms in the promoter region of HBsAg. The amino acid substitution of T2768G was associated with the accumulation of HBsAg. These observations may explain the HBsAg-associated hepatocarcinogenesis.
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