Analysis of the replication mechanism of hepatitis C virus in membrane vesicles containing replication complexes

• Project/Area Number: 15K09034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Hideki Aizaki 国立感染症研究所, ウイルス第二部, 室長 (00333360)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: HCV / プラス鎖RNAウイルス / 複製複合体 / Double membrane vesicle / SURF4 / 複製 / 膜小胞 / DMV / Surf4 / Replication / C型肝炎ウイルス / 宿主因子 / 小胞輸送

Outline of Final Research Achievements

Positive-strand RNA viruses such as hepatitis C virus (HCV), polio virus, and SARS coronavirus form membrane vesicles (Double membrane vesicles (DMV)) containing replication complexes. In this study, we analyzed the mechanism of membrane vesicle formation that is essential for HCV replication. It has been shown that the NS4B binding protein Surf4 may act to form a replication component common to positive-strand RNA viruses such as HCV and polio virus. These results indicate that Surf4 interacts with NS4B to form DMV and participate in HCV replication.

Academic Significance and Societal Importance of the Research Achievements

C型肝炎ウイルス(HCV)、ポリオウイルス、サーズコロナウイルス等のプラス鎖RNAウイルス共通の複製膜は正常なオルガネラには存在しない特徴滴な膜構造物と考えられることから、これらの疾患の診断、治療のターゲットとなりするものと期待できる。

Research Products

• [Journal Article] Epidermal growth factor receptor is a host entry cofactor triggering hepatitis B virus internalization (2019)
  • Author(s): M. Iwamoto, W. Saso, R. Sugiyama, K. Ishii, M. Ohki, S. Nagamori, R. Suzuki, H. Aizaki, A. Ryo, J-H Yun, S-Y Park, N. Ohtani, M. Muramatsu, S. Iwami, Y. Tanaka, C. Sureau, T. Wakita and K. Watashi
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 116 Issue: 17 Pages: 8487-8492
  • DOI: 10.1073/pnas.1811064116
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Activation of protein kinase R by hepatitis C virus RNA-dependent RNA polymerase. (2019)
  • Author(s): Suzuki R, Matsuda M, Shimoike T, Watashi K, Aizaki H, Kato T, Suzuki T, Muramatsu M, Wakita T.
  • Journal Title: Virology Volume: 529 Pages: 226-233
  • DOI: 10.1016/j.virol.2019.01.024
  • Peer Reviewed
• [Journal Article] Regulation of Hepatitis C Virus Infection by Cellular Retinoic Acid Binding Proteins through the Modulation of Lipid Droplet Abundance (2019)
  • Author(s): Bang Bo-Ram、Li Meng、Tsai Kuen-Nan、Aoyagi Haruyo、Lee Shin-Ae、Machida Keigo、Aizaki Hideki、Jung Jae U.、Ou Jing-Hsiung James、Saito Takeshi
  • Journal Title: Journal of Virology Volume: 93 Issue: 8
  • DOI: 10.1128/jvi.02302-18
  • Peer Reviewed
• [Journal Article] Troglitazone Impedes the Oligomerization of Sodium Taurocholate Cotransporting Polypeptide and Entry of Hepatitis B Virus Into Hepatocytes (2019)
  • Author(s): Fukano Kento、Tsukuda Senko、Oshima Mizuki、Suzuki Ryosuke、Aizaki Hideki、Ohki Mio、Park Sam-Yong、Muramatsu Masamichi、Wakita Takaji、Sureau Camille、Ogasawara Yuki、Watashi Koichi
  • Journal Title: Frontiers in Microbiology Volume: 9 Pages: 3257-3257
  • DOI: 10.3389/fmicb.2018.03257
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Thermostable hepatitis C virus JFH1-derived variant isolated by adaptation to Huh7.5.1 cells (2018)
  • Author(s): Shirasago Yoshitaka、Fukazawa Hidesuke、Aizaki Hideki、Suzuki Tetsuro、Suzuki Takeru、Sugiyama Kazuo、Wakita Takaji、Hanada Kentaro、Abe Ryo、Fukasawa Masayoshi
  • Journal Title: Journal of General Virology Volume: 99 Issue: 10 Pages: 1407-1417
  • DOI: 10.1099/jgv.0.001117
  • Peer Reviewed
• [Journal Article] A Single Adaptive Mutation in Sodium Taurocholate Cotransporting Polypeptide Induced by Hepadnaviruses Determines Virus Species Specificity (2018)
  • Author(s): Takeuchi Junko S.、Fukano Kento、Iwamoto Masashi、Tsukuda Senko、Suzuki Ryosuke、Aizaki Hideki、Muramatsu Masamichi、Wakita Takaji、Sureau Camille、Watashi Koichi
  • Journal Title: Journal of Virology Volume: 93 Issue: 5 Pages: 04132-18
  • DOI: 10.1128/jvi.01432-18
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] High-throughput neutralization assay for multiple flaviviruses based on single-round infectious particles using dengue virus type 1 reporter replicon. (2018)
  • Author(s): Matsuda M, Yamanaka A, Yato K, Yoshii K, Watashi K, Aizaki H, Konishi E, Takasaki T, Kato T, Muramatsu M, Wakita T, Suzuki R
  • Journal Title: Sci Rep Volume: 8 Issue: 1 Pages: 16624-16624
  • DOI: 10.1038/s41598-018-34865-y
  • Peer Reviewed / Open Access
• [Journal Article] The aryl hydrocarbon receptor?cytochrome P450 1A1 pathway controls lipid accumulation and enhances the permissiveness for hepatitis C virus assembly (2018)
  • Author(s): Ohashi Hirofumi、Nishioka Kazane、Nakajima Syo、Kim Sulyi、Suzuki Ryosuke、Aizaki Hideki、Fukasawa Masayoshi、Kamisuki Shinji、Sugawara Fumio、Ohtani Naoko、Muramatsu Masamichi、Wakita Takaji、Watashi Koichi
  • Journal Title: Journal of Biological Chemistry Volume: 293 Issue: 51 Pages: 19559-19571
  • DOI: 10.1074/jbc.ra118.005033
  • Peer Reviewed
• [Journal Article] A new strategy to identify hepatitis B virus entry inhibitors by AlphaScreen technology targeting the envelope-receptor interaction. (2018)
  • Author(s): Saso W, Tsukuda S, Ohashi H, Fukano K, Morishita R, Matsunaga S, Ohki M, Ryo A, Park SY, Suzuki R, Aizaki H, Muramatsu M, Sureau C, Wakita T, Matano T, Watashi K.
  • Journal Title: Biochem Biophys Res Commun Volume: 印刷中 Issue: 2 Pages: 374-379
  • DOI: 10.1016/j.bbrc.2018.04.187
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chemical array system, a platform to identify novel hepatitis B virus entry inhibitors targeting sodium taurocholate cotransporting polypeptide (2018)
  • Author(s): Kaneko M, Futamura Y, Tsukuda S, Kondoh Y, Sekine T, Hirano H, Fukano K, Ohashi H, Saso W, Morishita R, Matsunaga S, Kawai F, Ryo A, Park S-Y, Suzuki R, Aizaki H, Ohtani N, Sureau C, Wakita T, Osada H, Watashi K
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 2769-2769
  • DOI: 10.1038/s41598-018-20987-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nonstructural 5A Protein of Hepatitis C Virus Interferes with Toll-Like Receptor Signaling and Suppresses the Interferon Response in Mouse Liver. (2017)
  • Author(s): Tsutsumi T, Okushin K, Enooku K, Fujinaga H, Moriya K, Yotsuyanagi H, Aizaki H, Suzuki T, Matsuura Y, Koike K.
  • Journal Title: PLOS One Volume: 12 Issue: 1 Pages: 2017-2017
  • DOI: 10.1371/journal.pone.0170461
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Human Cathelicidin Compensates for the Role of Apolipoproteins in Hepatitis C Virus Infectious Particle Formation. (2016)
  • Author(s): Puig-Basagoiti F, Fukuhara T, Tamura T, Ono C, Uemura K, Kawachi Y, Yamamoto S, Mori H, Kurihara T, Okamoto T, Aizaki H, Matsuura Y.
  • Journal Title: J Virol. Volume: 90(19) Pages: 8464-77
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Bivalent vaccine platform based on Japanese encephalitis virus (JEV) elicits neutralizing antibodies against JEV and hepatitis C virus. (2016)
  • Author(s): Saga R, Fujimoto A, Watanabe N, Matsuda M, Hasegawa M, Watashi K, Aizaki H, Nakamura N, Tajima S, Takasaki T, Konishi E, Kato T, Kohara M, Takeyama H, Wakita T, Suzuki R.
  • Journal Title: Sci Rep. Volume: 27 Pages: 28688-28688
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Single-domain intrabodies against HCV Core inhibit viral propagation and Core-induced NF-κB Activation. (2016)
  • Author(s): Suzuki R, Saito K, Matsuda M, Sato M, Kanegae Y, Shi G, Watashi K, Aizaki H, Chiba J, Saito I, Wakita T, Suzuki T.
  • Journal Title: J Gen Virol. Volume: 97(4) Pages: 887-92
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Prolactin regulatory element binding protein is involved in hepatitis C virus replication compartment by interacting with NS4B. (2016)
  • Author(s): Kong L, Fujimoto A, Nakamura M, Aoyagi H, Matsuda M, Watashi K, Suzuki R, Arita M, Yamagoe S, Dohmae N, Suzuki T, Sakamaki Y, Ichinose S, Suzuki T, Wakita T, Aizaki H.
  • Journal Title: J Virol. Volume: 90(6) Pages: 3093-111
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A Novel Tricyclic Polyketide, Vanitaracin A, Specifically Inhibits the Entry of Hepatitis B and D Viruses by Targeting Sodium Taurocholate Cotransporting Polypeptide. (2015)
  • Author(s): Kaneko M, Watashi K, Kamisuki S, Matsunaga H, Iwamoto M, Kawai F, Ohashi H, Tsukuda S, Shimura S, Suzuki R, Aizaki H, Sugiyama M, Park SY, Ito T, Ohtani N, Sugawara F, Tanaka Y, Mizokami M, Sureau C, Wakita T.
  • Journal Title: J Virol. Volume: 89 Pages: 11945-53
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dysregulation of Retinoic Acid Receptor Diminishes Hepatocyte Permissiveness to Hepatitis B Virus Infection through Modulation of NTCP Expression. (2015)
  • Author(s): Tsukuda S, Watashi K, Iwamoto M, Suzuki R, Aizaki H, Okada M, Sugiyama M, Kojima S, Tanaka Y, Mizokami M, Li J, Tong S, Wakita T.
  • Journal Title: J Biol Chem. Volume: 27 Pages: 5673-84
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Targeting cellular squalene synthase, an enzyme essential for cholesterol biosynthesis, is a potential antiviral strategy against hepatitis C virus. (2015)
  • Author(s): Saito K, Shirasago Y, Suzuki T, Aizaki H, Hanada K, Wakita T, Nishijima M, Fukasawa M. Targeting cellular squalene synthase, an enzyme essential for cholesterol biosynthesis, is a potential antiviral strategy against hepatitis C virus.
  • Journal Title: J Virol. Volume: 89 Pages: 2220-32
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Alyl hydrocarbon receptor-cytochrome p450 1a1 pathway regulates hepatic lipid biosynthesis to maximize Hepatitis C virus production. (2018)
  • Author(s): Hirofumi Ohashi, Syo Nakajima, Sulyl Kim, Ryosuke Suzuki, Hideki Aizaki, Masayoshi Fukasawa, Shinji Kamisuki, Fumio Sugawara, Naoko Ohtani, Masamichi Muramatsu, Takaji Wakita, Koichi Watashi.
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses.
  • Int'l Joint Research
• [Presentation] Construction of chimeric reporter HCV with efficient production capacity. (2018)
  • Author(s): Rika Sato, Noriyuki Watanabe, Hussein Aly, Madoka Koyanagi, Yutaka Arimura, Hideki Aizaki, Masamichi Muramatsu, Takaji Wakita.
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Establishment of infectious genotype 4a HCVcc. (2018)
  • Author(s): Noriyuki Watanabe, Takaya Suzuki, Tomoko Date, Su Su Hmwe, Hussein Aly, Hideki Aizaki, Masaya, Sugiyama, Masashi Mizokami, Mohamed El Kassas, Ashraf Tabll, William Delaney, Guofeng Cheng, Masamichi Muramatsu, Takaji Wakita.
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses.
  • Int'l Joint Research
• [Presentation] Antiviral activity of phospholipase A2 group V (PLA2G5) against HCV. (2018)
  • Author(s): Francesc Puig-Basagoiti, Masayoshi Fukasawa, Ryosuke Suzuki, Koichi Watashi, Masamichi Muramatsu, Takaji Wakita, Hideki Aizaki.
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses.
  • Int'l Joint Research
• [Presentation] Flavivirus subviral particles-based DNA vaccine induces neutralizing antibodies against HCV. (2018)
  • Author(s): Keigo Yato, Mami Matsuda, Noriyuki Watanabe, Shogo Nakajima, Akira Fujimoto, Koichi Watashi, Hideki Aizaki, Takanobu Kato, Koji Tamura, Masamichi Muramatsu, Takaji Wakita, Ryosuke Suzuki.
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses.
  • Int'l Joint Research
• [Presentation] Identification of Alyl hydrocarbon receptor ligands inhibiting the lipid accumulation and hepatitis C virus production. (2018)
  • Author(s): Kazane Nishioka, Hirofumi Ohashi, Ryosuke Suzuki, Hideki Aizaki, Masamichi Muramatsu, Takaji Wakita, Koichi Watashi.
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses.
  • Int'l Joint Research
• [Presentation] Abnormal hepatocellular organelles remain to be observed in sustained virological response (SVR) patients. (2018)
  • Author(s): Haruyo Aoyagi, Hiroko Iijima, Xin Zheng, Yu Ting Kao, Mami Matsuda, Koichi Watashi, Ryosuke Suzuki, Takahiro Masaki, Noritomo Shimada, Keizo Kato, Akihito Tsubota, Kenjiro Wake, Takaji Wakita, Hideki Aizaki.
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses.
  • Int'l Joint Research
• [Presentation] Abnormal hepatocellular organelles remain to be observed in sustained virological response patients. (2018)
  • Author(s): Haruyo Aoyagi, et. al.
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses.
  • Int'l Joint Research
• [Presentation] Human hepatic stellate cells are permissive for hepatitis C virus infection/replication and play important roles in fibrosis. (2018)
  • Author(s): Xin Zheng, et al.
  • Organizer: 25th International Symposium on Hepatitis C Virus and Related Viruses.
  • Int'l Joint Research
• [Presentation] Epidemiology Study of Acute Hepatitis B and C in Japan, from April 1999 (2018)
  • Author(s): Xin Zheng, Haruyo Aoyagi, Gewaid E. Hossam, Takuma Zaitsu, Francesc Puig-Basagoiti, Yu Ting Kao, Koichi Watashi, Ryosuke Suzuki, Takuri Takahashi, Tomimasa Sunagawa, Kazunori Oishi, Takaji Wakita, Hideki Aizaki.
  • Organizer: 6th JAPAN-TAIWAN-KOREA HBV Research Symposium 2018
• [Presentation] HCVに対する抗ウイルス治療後、SVR後の肝細胞の超微細構造の変化. (2018)
  • Author(s): 青柳東代, 飯島尋子, 松田麻未, 渡士幸一, 鈴木亮介, 政木隆博, 酒巻有里子, 市野瀬志津子, 坪田昭人, 和氣健二郎, 脇田隆字, 相崎英樹.
  • Organizer: 第２６回抗ウイルス療法学会総会
• [Presentation] Hepatitis C virus infection triggers the transavtivation of alyl hydrocarbon receptor to rearrange hepatic lipid biosynthesis. (2018)
  • Author(s): Hirofumi Ohashi, Syo Nakajima, Sulyl Kim, Ryosuke Suzuki, Hideki Aizaki, Masayoshi Fukasawa, Shinji Kamisuki, Fumio Sugawara, Naoko Ohtani, Masamichi Muramatsu, Takaji Wakita, Koichi Watashi.
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] Development and use of chronological and real-time monitoring system of hepatitis C virus RNA replication. (2018)
  • Author(s): Xiaoyu Li, Masahiko Ito, Kenji Nakashima, Haruyo Aoyagi, Hideki Aizaki, Tetsuro Suzuki.
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] HCV genome and abnormal organelles in the liver after elimination of hepatitis C virus by drug treatment. (2018)
  • Author(s): Hideki Aizaki, Haruyo Aoyagi, Hiroko Iijima, Xin Zheng, Mami Matsuda, Koichi Watashi, Ryosuke Suzuki, Takahiro Masaki, Noritomo Shimada, Keizo Kato, Akihito Tsubota, Takeshi Saito, Kazuhiko Hayashi, Masaru Enomoto, Ayako Mimata, Yuriko Sakamaki, Shizuko Ichinose, Kenjiro Wake, Masamichi Muramatsu, Takaji Wakita.
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] Identification of Alyl hydrocarbon receptor ligands inhibiting the lipid accumulation and hepatitis C virus production. (2018)
  • Author(s): Kazane Nishioka, Hirofumi Ohashi, Ryosuke Suzuki, Hideki Aizaki, Masamichi Muramatsu, Takaji Wakita, Koichi Watashi.
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] Construction of chimeric reporter HCV efficient production capacity. (2018)
  • Author(s): Rika Sato, Noriyuki Watanabe, Hussein Aly, Madoka Koyanagi, Yutaka Arimura, Hideki Aizaki, Masamichi Muramatsu, Takaji Wakita.
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] Establishment of infectious genotype 4a HCVcc. (2018)
  • Author(s): Noriyuki Watanabe, Takaya Suzuki, Tomoko Date, Su Su Hmwe, Hussein Aly, Masaya Sugiyama, Masashi Mizokami, Hideki Aizaki, Masamichi Muramatsu, Takaji Wakita.
  • Organizer: 第66回日本ウイルス学会学術集会
• [Presentation] Ultrastructure of hepatocytes in chronic hepatitis C patients who achieve a sustained virological response. (2017)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Zheng X, Kao YT, Hossam GE, Zaitsu T, Matsuda M, Watashi K, Suzuki S, Masaki T, Shimada N, Kato K, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H.
  • Organizer: The Asian Pacific Association for the Study of the Liver
  • Place of Presentation: China
  • Year and Date: 2017-02-16
  • Int'l Joint Research
• [Presentation] Phosphorylation of protein kinase R and translational suppression by NS5B protein of hepatitis C virus. (2017)
  • Author(s): Suzuki R, Matsuda M, Shimoike T, Watashi k, Aizaki H, Kato T, Suzuki T, Wakita T.
  • Organizer: 日本ウイルス学会第65回学術集会
• [Presentation] Generation of hepatitis C virus vaccine antigen based on Japanese encephalitis virus subviral particles. (2017)
  • Author(s): Yato K, Matsuda M, Watanabe N, Nakajima S, Fujimoto A, Watashi K, Aizaki H, Kato T, Tamura K, Wakita T, Suzuki R.
  • Organizer: 日本ウイルス学会第65回学術集会
• [Presentation] Membrane protein, embryonic lethal, abnormal vision, drosophila-like 1, interacts with NS5A and involves in hepatitis C virus replication (2017)
  • Author(s): Kao YT, Goto K, Aoyagi H, Hossam GE, Zheng X, Puig-Basagoiti F, Watashi K, Suzuki R, Yamagoe S, Dohmae N, Suzuki T, Okushin K, Tsutsumi T, Yotsuyanagi H, Moriya K, Koike K, Suzuki T, Wakita T, Aizaki H
  • Organizer: 日本ウイルス学会第65回学術集会
• [Presentation] Novel crosstalk of Xenobiotic Response with Lipid Biosynthesis Pathway regulates the Host Permissiveness to the Hepatitis C Virus Production (2017)
  • Author(s): Ohashi H, Nakajima S, Kim S, Fukasawa M, Suzuki R, Aizaki H, Sugawara F, Ohtani N, Wakita T, Watashi K
  • Organizer: 日本ウイルス学会第65回学術集会
• [Presentation] Human hepatic stellate cells are permissive for hepatitis C virus infection/replication and play important roles in fibrosis (2017)
  • Author(s): Zheng X, Zaitsu T, Aoyagi H, Matsuda M, Watanabe N, Fujimoto A, Watashi K, Suzuki R, Fukuhara T, Matsuura Y, Ichinose S, Wake K, Suzuki T, Iijima H, Yokoyama H, Masaki T, Matsuura T, Tamura K, Wakita T, Aizaki H
  • Organizer: 日本ウイルス学会第65回学術集会
• [Presentation] Abnormal hepatocellular organelles remain to be observed in sustained virological response patients (2017)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Zheng X, Kao YT, Hossam GE, Matsuda M, Watashi K, Suzuki R, Masaki T, Aizawa N, Shimada N, Kato K, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H
  • Organizer: 日本ウイルス学会第65回学術集会
• [Presentation] C型慢性肝炎のウイルス学的著効（SVR）後の肝実質細胞のオルガネラ異常の解析 (2017)
  • Author(s): 青柳東代, 飯島尋子, Francesc Puig-Basagoiti, Zheng Xin, Yu Ting Kao, Gewaid E. Hossam, 在津拓馬, 松田麻未, 渡士幸一, 鈴木亮介, 政木隆博, 島田紀明, 加藤慶三, 坪田昭人, 三又絢子, 酒巻有里子, 市野瀬志津子, 和氣健二郎, 脇田隆字, 相崎英樹
  • Organizer: 第27回抗ウイルス療法学会学術集会・総会
• [Presentation] HCVの肝星細胞への感染および肝星細胞活性化のメカニズム解析 (2017)
  • Author(s): 相崎英樹、渡士幸一、鈴木哲朗、政木隆博、横山寛、脇田隆字
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] Crosstalk of Xenobiotic Metabolism Pathway with Lipid Metabolism regulates the Host Permissiveness to Hepatitis C Virus Production (2017)
  • Author(s): Ohashi H, Nakajima S, Kim S, Suzuki R, Aizaki H, Fukasawa M, Kamisuki S, Sugawara F, Ohtani N, Wakita T, Watashi K
  • Organizer: 第14回ウイルス学キャンプin湯河原
• [Presentation] Abnormal organelles were continuously observed in sustained virological response (SVR) patients (2017)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Zheng X, Kao YT, Gewaid HE, Zaitsu T, Matsuda M, Watashi K, Suzuki R, Masaki T, Shimada N, Kato K, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H
  • Organizer: The Asian Pacific Associaton for the Study of the Liver - Single Topic Conference 2017
  • Int'l Joint Research
• [Presentation] Acute hepatitis C virus infection among HIV-infected men who have sex with men (2017)
  • Author(s): Aoyagi H, Itoda I, Zheng X, Hossam GE, Puig-Basagoiti F, Kao YT, Watashi K, Suzuki R, Wakita T, Aizaki H
  • Organizer: APASL Single Topic Conference 2017 Mongolia 6th HCV Conference on HCV and CO-INFECTIONS
  • Int'l Joint Research
• [Presentation] Sphingomyelin participates in the formation of Hepatitis C virus membrane replication vesicles (2017)
  • Author(s): Gewaid HE, Aoyagi H, Arita M, Watashi K, Suzuki R, Aly HH, Kumagai K, Yamaji T, Fukasawa M, Mimata A, Sakamaki Y, Ichinose S, Hanada K, Wakita T, Aizaki H
  • Organizer: American Society for Virology - 2017 Annual Meeting
  • Int'l Joint Research
• [Presentation] Structural proteins of hepatitis C virus Genotype 3a S310 strain permit claudin-1-independent entry. (2017)
  • Author(s): Suzuki R, Yato K, Tosaka T, Su Su Hmwe, Matsuda M, Watashi K, Aizaki H, Fukasawa M, Matsuura Y, Wakita T
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses.
  • Int'l Joint Research
• [Presentation] NS5A-Associated Membrane Protein, Embryonic Lethal, Abnormal Vision, Drosophila-Like 1, Involves in Hepatitis C Replication (2017)
  • Author(s): Kao YT, Goto K, Aoyagi H, Hossam GE, Zheng X, Puig-Basagoiti F, Watashi K, Suzuki R, Yamagoe S, Dohmae N, Suzuki T, Okushin K, Tsutsumi T, Yotsuyanagi H, Moriya K, Koike K, Suzuki T, Wakita T, Aizaki H
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Novel Crosstalk of Xenobiotic Response with Lipid Metabolism Regulates the Host Permissivenessto Hepatitis C Virus Production (2017)
  • Author(s): Ohashi H, Nakajima S, Kim S, Suzuki R, Aizaki H, Fukasawa M, Kamisuki S, Sugawara F, Ohtani N, Wakita T, Watashi K
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Sphingomyelin participates in the formation of Hepatitis C virus membrane replication vesicles. (2017)
  • Author(s): Gewaid HE, Aoyagi H, Kao YU, Zheng X, Puig-Basagoiti F, Watashi K, Arita M, Suzuki R, Aly HH, Kumagai K, Yamaji T, Fukasawa M, Mimata A, Sakamaki Y, Ichinose S, Hanada K, Wakita T, Aizaki H
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] HCVに対する抗ウイルス治療後、SVR後の肝細胞の超微細構造の変化 (2016)
  • Author(s): 青柳東代、飯島尋子、松田麻未、渡士幸一、鈴木亮介、會澤信弘、政木隆博、坪田昭人、和氣健二郎、脇田隆字、相崎英樹
  • Organizer: 第41回日本肝臓学会東部会
  • Place of Presentation: 東京
  • Year and Date: 2016-12-08
• [Presentation] Aryl hydrocarbon receptor regulates the assembly of hepatitis C virus and affects the emergence of drug resistant virus against DAAs. (2016)
  • Author(s): Ohashi H, Nakajima S, Kim S, Suzuki R, Aizaki H, Fukasawa M, Kamisuki S, Sugawara F, Ohtani N, Wakita T, Watashi K.
  • Organizer: 日本ウイルス学会第64回学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2016-10-23
• [Presentation] Sphingomyelin is a component in the membranous replication factories. (2016)
  • Author(s): Gewaid HE, Aoyagi H, Watashi K, Suzuki R, Aly H, Kumagai K, Yamaji T, Fukasawa M, Mimata A, Sakamaki Y, Ichinose S, Hanada K, Wakita T, Aizaki H.
  • Organizer: 23rd International Symposium on Hepatitis C Virus and Related Viruses.
  • Place of Presentation: Japan
  • Year and Date: 2016-10-11
  • Int'l Joint Research
• [Presentation] Human hepatic stellate cells are permissive for hepatitis C virus infection/replication and play important roles in fibrosis. (2016)
  • Author(s): Zaitsu T, Aoyagi H, Matsuda M, Watanabe N, Fujimoto A, Watashi K, Suzuki R, Fukuhara T, Matsuura Y, Wake K, Suzuki T, Matsuura T, Tamura K, Wakita T, Aizaki H.
  • Organizer: 23rd International Symposium on Hepatitis C Virus and Related Viruses. Kyoto
  • Place of Presentation: Japan
  • Year and Date: 2016-10-11
  • Int'l Joint Research
• [Presentation] Activation of protein kinase R by hepatitis C virus RNA-dependent RNA polymerase. (2016)
  • Author(s): Suzuki R, Matsuda M, Shimoike T, Watashi K, Aizaki H, Kato T, Suzuki T, Wakita T.
  • Organizer: 23rd International Symposium on Hepatitis C Virus and Related Viruses.
  • Place of Presentation: Japan
  • Year and Date: 2016-10-11
  • Int'l Joint Research
• [Presentation] Role of lipid droplets in the emergence of drug resistant virus against direct acting antivirals. (2016)
  • Author(s): Ohashi H, Nakajima S, Kim S, Suzuki R, Aizaki H, Fukasawa M, Kamisuki S, Sugawara F, Ohtani N, Wakita T, Watashi K.
  • Organizer: 23rd International Symposium on Hepatitis C Virus and Related Viruses.
  • Place of Presentation: Japan
  • Year and Date: 2016-10-11
  • Int'l Joint Research
• [Presentation] Ultrastructure of hepatocytes in chronic hepatitis C patients who achieve a sustained virological response. (2016)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Zheng X, Kao YT,. Hossam GE, Zaitsu T, Matsuda M, Watashi K, Suzuki R, Masaki T, Shimada N, Kato K, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H.
  • Organizer: The 15th Awaji International Forum on Infection and Immunity.
  • Place of Presentation: Japan
  • Year and Date: 2016-09-06
  • Int'l Joint Research
• [Presentation] スフィンゴ脂質のC型肝炎ウイルス複製における役割の解析. (2016)
  • Author(s): ホッサムゲワイド、口頭、青柳東代、渡士幸一、鈴木亮介、熊谷圭悟、山地俊之、深澤征義、酒巻有里子、市野瀬志津子、花田賢太郎、脇田隆字、相崎英樹.
  • Organizer: 第５８回日本脂質生化学学会
  • Place of Presentation: 秋田
  • Year and Date: 2016-06-09
• [Presentation] HCVに対する抗ウイルス治療後、SVR後の肝細胞の超微細構造の変化. (2016)
  • Author(s): 青柳東代、飯島尋子、松田麻未、渡士幸一、鈴木亮介、政木隆博、坂巻有里子、市野瀬志津子、坪田昭人、和氣健二郎、脇田隆字、相崎英樹
  • Organizer: 第26回抗ウイルス療法学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-05-13
• [Presentation] Surfeit 4 is involved in HCV replication by interacting with NS4B. Positive-strand RNA viruses (2016)
  • Author(s): Kong L, Aoyagi H, Matsuda M, Fujimoto A, Watashi K, Suzuki R, Suzuki T, Wakita T, Aizaki1 H,
  • Organizer: Plus strand RNA meeting
  • Place of Presentation: USA
  • Year and Date: 2016-05-02
  • Int'l Joint Research
• [Presentation] Prolactin regulatory element binding protein and Surfeit 4 are involved in hepatitis C virus replication compartment by interacting with NS4B. (2016)
  • Author(s): Kong L, Fujimoto A, Nakamura M, Aoyagi H, Matsuda M, Watashi K, Suzuki R, Arita M, Yamagoe S, Dohmae N, Suzuki T, Sakamaki Y, Ichinose S, Suzuki T, Wakita T, Aizaki H.
  • Organizer: Asian Pacific Association for the Study of the Liver
  • Place of Presentation: 東京
  • Year and Date: 2016-02-20
  • Int'l Joint Research
• [Presentation] 感染細胞の超微形態変化（SVR 後も含めて） (2016)
  • Author(s): 相崎英樹
  • Organizer: (公財)宮川庚子記念研究財団研修会
  • Place of Presentation: 東京
  • Year and Date: 2016-02-13
  • Invited
• [Presentation] HCVに対する抗ウイルス治療後、SVR後の肝細胞の超微細構造の変化 (2016)
  • Author(s): 青柳東代、飯島尋子、松田麻未、渡士幸一、鈴木亮介、政木隆博、三又絢子、酒巻有里子、市野瀬志津子、坪田昭人、和氣健二郎、脇田隆字、相崎英樹
  • Organizer: 広島肝臓プロジェクト研究センターシンポジウム
  • Place of Presentation: 広島
• [Presentation] 肝炎ウイルス検査陽性者アンケート調査の受診勧奨効果 (2015)
  • Author(s): 中野卓二、橋本千樹、川部直人、村尾道人、嶋崎宏明、菅敏樹、中岡和徳、高川友花、大城昌史、高村知希、野村小百合、吉岡健太郎、中田佳穂里、青山政美、片岡博喜、相崎英樹、是永匡紹.
  • Organizer: 第41回日本肝臓学会西部会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-12-03
• [Presentation] Development of a novel vaccine platform based on Japanese encephalitis viruses. (2015)
  • Author(s): Suzuki R, Saga R, Fujimoto A, Watanabe N, Matsuda M, Hasegawa M, Watashi K, Aizaki H, Nakamura N, Konishi E, Kato T, Tajima S, Takasaki T, Takeyama H, Wakita T.
  • Organizer: 日本分子生物学会第38回年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Surfeit 4 is involved in HCV replication by interacting with NS4B. (2015)
  • Author(s): Lingbao K, Aoyagi H, Matsuda M, Fujimoto A, Watashi K, Suzuki R, Yamagoe S, Dohmae N, Sakamaki Y, Ichinose S, Suzuki T, Wakita T, Aizaki H.
  • Organizer: 日本ウイルス学会第63回学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-22
• [Presentation] A new fungi-derived vanitaracin A specifically inhibits the entry of hepatitis B and D viruses through interacting with NTCP. (2015)
  • Author(s): Kaneko M, Watashi K, Kamisuki S, Matsunaga H, Iwamoto M, Kawai H, Ohashi H, Tsukuda S, Shimura S, Suzuki R, Aizaki H, Sugiyama M, Sam-Yong P, Ohtani N, Sugawara F, Tanaka Y, Mizokami M, Wakita T.
  • Organizer: 日本ウイルス学会第63回学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-22
• [Presentation] Transfer Hepatitis C virus from human liver cells to hepatic stellate cells. (2015)
  • Author(s): Zaitsu T, Aoyagi H, Fujimoto A, Matsuda M, Watanabe N, Watashi K, Suzuki R, Fukuhara T, Matsuura Y, Sakamaki Y, Ichinose S, Wake K, Suzuki T, Iijima H, Matsuura T, Tamura K, Wakita T, Aizaki H.
  • Organizer: 日本ウイルス学会第63回学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-22
• [Presentation] Multimerization of hepatitis B virus core protein regulated by microtubules. (2015)
  • Author(s): Iwamoto M, Watashi K, Sugiyama M, Suzuki R, Aizaki H, Tanaka Y, Mizokami M, Ohtani N, Koiwai O, Wakita T.
  • Organizer: 日本ウイルス学会第63回学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-22
• [Presentation] Identification of a flavonoid derivative that directly acts on hepatitis B virus surface protein and inhibits viral entry. (2015)
  • Author(s): Tsukuda S, Watashi K, Hamada Y, Isogawa M, Iwamoto M, Suzuki R, Aizaki H, Kojima S, Sugiyama M, Saito A, Tanaka Y, Mizokami M, Wakita T.
  • Organizer: 日本ウイルス学会第63回学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-22
• [Presentation] Bivalent vaccine platform based on Japanese encephalitis virus (JEV) elicit neutralizing antibodies against JEV and Hepatitis C virus. (2015)
  • Author(s): Saga R, Fujimoto A, Watanabe N, Matsuda M, Hasegawa M, Watashi K, Aizaki H, Nakamura N, Konishi E, Kato T, Tajima S, Takasaki T, Takeyama H, Wakita T, Suzuki R.
  • Organizer: 日本ウイルス学会第63回学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-22
• [Presentation] Flutamide-mediated Disruption of Lipid Droplets Impairs the Host Permissiveness to Hepatitis C virus Production. (2015)
  • Author(s): Ohashi H, Watashi K, Nakajima S, Sulyi K, Suzuki R, Aizaki H, Fukasawa M, Kamisuki S, Sugawara F, Ohtani N, Wakita T.
  • Organizer: 日本ウイルス学会第63回学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-22
• [Presentation] Transfer hepatitis C virus between human liver cells and hepatic stellate cells. (2015)
  • Author(s): Aizaki H.
  • Organizer: The 2015 Italy-Japan Liver Workshop
  • Place of Presentation: Italy
  • Year and Date: 2015-10-26
  • Int'l Joint Research
• [Presentation] Japanese encephalitis virus (JEV)-subviral particles harboring HCV neutralization epitopes elicit neutralizing antibodies against JEV and HCV. (2015)
  • Author(s): Saga R, Fujimoto A, Watanabe N, Matsuda M, Hasegawa M, Watashi K, Aizaki H, Nakamura N, Konishi E, Kato T, Takeyama H, Wakita T, Suzuki R.
  • Organizer: 22nd International Symposium on Hepatitis C Virus and Related Viruses.
  • Place of Presentation: Strasbourg, France
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] Identification of NTCP-targeting small molecules that inhibit HBV entry by cell-based and in vitro technologies. (2015)
  • Author(s): Watashi K, Tsukuda S, Shimura S, Kaneko M, Sluder A, Peel M, Kamisuki S, Matsunaga S, Morishita R, Futamura Y, Kondoh Y, Suzuki R, Aizaki H, Sugiyama M, Borroto-Esoda K, Ryo A, Sugawara F, Osada H, Tanaka Y, Mizokami M, Wakita T.
  • Organizer: 2015 International Meeting, Molecular Biology of Hepatitis B Viruses.
  • Place of Presentation: Dolce Bad Nauheim, Germany
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Cilnidipine inhibits the early phase of HBV infection in a NTCP independent mechanism. (2015)
  • Author(s): Tsukuda S, Watashi K, Iwamoto M, Suzuki R, Aizaki H, Tanaka Y, Wakita T.
  • Organizer: 2015 International Meeting, Molecular Biology of Hepatitis B Viruses.
  • Place of Presentation: Dolce Bad Nauheim, Germany
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Identification of a new tricyclic polyketide, vanitaracin A, which inhibits the entry of HBV, HDV, and nucleoside analog-resistant HBV through targeting NTCP. (2015)
  • Author(s): Kaneko M, Watashi K, Kamisuki S, Mtsunaga H, Iwamoto M, Kawai F, Ohashi H, Tsukuda S, Shimura S, Suzuki R, Aizaki H, Sugiyama M, Sam-Yong P, Ohtani N, Sugawara F, Tanaka Y, Mizokami M, Sureau C, Wakita T.
  • Organizer: 2015 International Meeting, Molecular Biology of Hepatitis B Viruses.
  • Place of Presentation: Dolce Bad Nauheim, Germany
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Prolactin regulatory element binding protein promotes the formation of HCV membranes replication compartment by interacting with NS4B. (2015)
  • Author(s): Kong L, Fujimoto A, Aoyagi H, Matsuda M, Watashi K, Suzuki R, Yamagoe S, Dohmae N, Suzuki T, Suzuki T, Wakita T, Aizaki H.
  • Organizer: 22nd International Symposium on Hepatitis C Virus and Related Viruses.
  • Place of Presentation: Strasbourg, France
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] B型肝炎ウイルスに対する直接標的薬proanthocyanidinおよび宿主標的薬vanitalacinの感染阻害効果および作用機序の解析. (2015)
  • Author(s): 渡士幸一、九十田千子、金子学、松永大輝、紙透伸治、齊藤安貴子、鈴木亮介、相崎英樹、杉山真也、溝上雅史、菅原二三男、田中靖人、脇田隆字.
  • Organizer: 第25回抗ウイルス療法学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-22
• [Presentation] Neoechinulin Bのliver X receptor転写阻害効果の同定およびそれによるC型肝炎ウイルス複製制御機構の解析. (2015)
  • Author(s): 渡士幸一、中嶋翔、紙透伸治、Jesus Izaguirre-Carbonell、Andrew Tae-Jun Kwon、鈴木治和、九十田千子、岡田麻衣子、鈴木亮介、相崎英樹、菅原二三男、脇田隆字
  • Organizer: 第25回抗ウイルス療法学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-22
• [Presentation] 新規真菌二次代謝産物 SF177 の B 型肝炎ウイルス感染阻害活性および NTCP トランスポーター阻害活性の同定. (2015)
  • Author(s): 金子学, 渡士幸一，紙透伸治, 岩本将士，河合文啓, 鈴木亮介，相崎英樹，朴三用, 菅原二三男, 脇田隆字.
  • Organizer: 第25回抗ウイルス療法学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-22
• [Presentation] HCV複製複合体を含む膜小胞の形成メカニズムの解析 (2015)
  • Author(s): 相崎英樹、脇田隆字.
  • Organizer: 第51回日本肝臓学会総会
  • Place of Presentation: 熊本
  • Year and Date: 2015-05-21
• [Book] C型肝炎ウイルスの遺伝子構造と抗ウイルス薬の作用機序は？ (2018)
  • Author(s): 52.相崎英樹
  • Total Pages: 1
  • Publisher: 日本医事新報
• [Book] 特集ウイルス肝炎のすべて、医学のあゆみ (2017)
  • Author(s): 4.相崎英樹、Zheng Xin、脇田隆字
  • Total Pages: 6
  • Publisher: 医歯薬出版社
• [Book] ここまでわかったC型肝炎ウイルスの感染・複製機構、目覚しく治療効果を発揮するC型肝炎治療 (2017)
  • Author(s): 相崎英樹、和気健二郎、脇田隆字
  • Total Pages: 10
  • Publisher: メジカルビュー社
• [Book] Mebio (2017)
  • Author(s): 相崎英樹、和気健二郎、脇田隆字
  • Total Pages: 10
  • Publisher: メジカルビュー社
• [Book] 医薬ジャーナル (2016)
  • Author(s): 相崎英樹、脇田隆字
  • Total Pages: 4
  • Publisher: 医薬ジャーナル社
• [Book] くらしの豆知識 (2016)
  • Author(s): 相崎英樹、脇田隆字
  • Total Pages: 2
  • Publisher: 国民生活センター
• [Book] 肝炎ウイルス検査のすすめ (2016)
  • Author(s): 相崎英樹、脇田隆字
  • Total Pages: 2
  • Publisher: 国民生活センター
• [Book] C型肝炎ウイルスの複製・増殖のメカニズム、特集C型肝炎治療における新時代の幕開け、特集C型肝炎治療における新時代の幕開け (2015)
  • Author(s): 相崎英樹、脇田隆字
  • Total Pages: 4
  • Publisher: 医薬ジャーナル社
• [Book] 日本におけるC型肝炎の疫学国際比較、真ウイルス性肝炎学―最新の基礎・臨床研究情報― (2015)
  • Author(s): 田中純子、相崎英樹、内田茂治
  • Total Pages: 9
  • Publisher: 日本臨床社