| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The aims of this study were to test whether HCV affects the ability of microRNAs (miRNAs), including miR-122, to repress their endogenous targets, and if so, to address the underlying mechanism(s). The results showed that HCV infection led to increases in the expression of endogenous miRNA targets. This de-repression of miRNA targets was canceled in HCV subgenomic replicon (SGR) cells by supplementation of exogenous mature miRNAs. However, miRNA functions remained impaired in HCV-infected cells, suggesting the possible involvement of viral structural proteins in miRNA dysfunction. Interestingly, this miRNA dysfunction was reproduced in SGR cells by knocking down some of miRNA-induced silencing complex (miRISC) proteins, while overexpression of these proteins restored miRNA functions in HCV-infected cells. These results suggest that HCV structural proteins may induce the dysfunction of miRISC, resulting in the global de-repression of miRNA targets.
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