microRNA expression during the multiple steps of pancreatic carcinogenesis

• Project/Area Number: 15K09038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAMOTO Natsuyo 東京大学, 医学部附属病院, 登録研究員 (40599578)
• Co-Investigator(Kenkyū-buntansha): 伊地知 秀明 東京大学, 医学部附属病院, 講師 (70463841) ; 大塚 基之 東京大学, 医学部附属病院, 講師 (90518945)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵発癌 / microRNA / スプライシング / 膵がん / 膵癌

Outline of Final Research Achievements

We determined the microRNA expresssion profiles in normal pnacreas tissues, PanIN tissues, and pancreatic carcinoma tissues. We found taht miR125b is increasing proportionally to the pancreatic carcinogenetic steps. In addition, miR125b trasnlocated into the nucleus when culturing the cells in three dimension and we found Galectin 3 binds to the miR125b, which may be involved in the translocation of this microRNA.Furthermore, miR125b in thenucleus was involved in the splicing of mRNAs dependeing on the sequences. These findings may be related with the pancreatic carcinogenesis by a novel pathway.

Research Products

• [Journal Article] Biliary epithelial injury-induced regenerative response by IL-33 promotes cholangiocarcinogenesis from peribiliary glands. (2017)
  • Author(s): Nakagawa H, Suzuki N, Hirata Y, Hikiba Y, Hayakawa Y, Kinoshita H, Ihara S, Uchino K, Nishikawa Y, Ijichi H, Otsuka M, Arita J, Sakamoto Y, Hasegawa K, Kokudo N, Tateishi K, Koike K.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 114(19) Issue: 19
  • DOI: 10.1073/pnas.1619416114
  • Peer Reviewed / Open Access
• [Journal Article] Repression of miRNA function mediates inflammation-associated colon tumorigenesis. (2017)
  • Author(s): Yoshikawa T., J. F. Wu, M. Otsuka, T. Kishikawa, N. Suzuki, A. Takata, M. Ohno, R. Ishibashi, M. Yamagami, R. Nakagawa, N. Kato, M. Miyazawa, J. Han, and K. Koike
  • Journal Title: Gastroenterology Volume: 152 Issue: 3 Pages: 631-643
  • DOI: 10.1053/j.gastro.2016.10.043
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Thromboembolisms in Advanced Pancreatic Cancer: A Retrospective Analysis of 475 Patients. (2017)
  • Author(s): Ishigaki K(1), Nakai Y, Isayama H, Saito K, Hamada T, Takahara N, Mizuno S, Mohri D, Kogure H, Matsubara S, Yamamoto N, Tada M, Koike K.
  • Journal Title: Pancreas Volume: 46 Issue: 8 Pages: 1069-1075
  • DOI: 10.1097/mpa.0000000000000889
  • Peer Reviewed / Open Access
• [Journal Article] Outcome of long-term maintenance steroid therapy cessation in patients with autoimmune pancreatitis (2015)
  • Author(s): Hirano K, Tada M, Isayama H, Sasahira N, Umefune G, Akiyama D, Watanabe T, Saito T, Takagi K, Takahara N, Hamada T, Mizuno S, Miyabayashi K, Mohri D, Kogure H, Yamamoto N, Nakai Y, Arizumi T, Toda N, Koike K
  • Journal Title: J Clin Gastroenterol. Volume: 50 Issue: 4 Pages: 331
  • DOI: 10.1097/mcg.0000000000000440
  • Peer Reviewed
• [Journal Article] Pancreatic cancer with malignant ascites: clinical features and outcomes (2015)
  • Author(s): Takahara N, Isayama H, Nakai Y, Sasaki T, Saito K, Hamada T, Mizuno S Miyabayashi K, Mohri D, Kogure H, Matsubara S, Yamamoto N, Hirano K, Ijichi H, Tateishi K, Tada M, Koike K.,
  • Journal Title: Pancreas. Volume: 44 Issue: 3 Pages: 380-385
  • DOI: 10.1097/mpa.0000000000000290
  • Peer Reviewed