Identification of a molecular marker relating to the progression of pancreatic cancer cells

• Project/Area Number: 15K09042
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: The University of Tokyo
• Principal Investigator: Nakai Yousuke 東京大学, 医学部附属病院, 助教 (80466755)
• Co-Investigator(Kenkyū-buntansha): 山本 恵介 東京大学, 医学部附属病院, 助教 (10608532) ; 立石 敬介 東京大学, 医学部附属病院, 講師 (20396948) ; 松原 三郎 東京大学, 医学部附属病院, 助教 (40750550)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 膵癌 / KDM6B / 膵がん / エピジェネテイクス

Outline of Final Research Achievements

We reported a novel role of KDM6B in regulating PDAC progression (Carcinogenesis 2014;35(11):2404-14). KDM6B was downregulated in high grade PDACs and knockdown of KDM6B in PDAC cells increased the tumorigenicity and enhanced the aggressive phenotypes in vivo. Furthermore, CEBPA was identified as a direct target of KDM6B, and reduced KDM6B- C/EBP　axis was resulted in increased aggressiveness in PDAC cells. We tried to identify a surrogate molecular marker of the cells lacking KDM6B- function. We used a cDNA　microarray to compare the expression profiles of KDM6B- KD and control PDAC cells and identified the candidate molecule relating to the loss of KDM6B.

Research Products

• [Journal Article] Thromboembolisms in Advanced Pancreatic Cancer: A Retrospective Analysis of 475 Patients. (2017)
  • Author(s): Ishigaki K(1), Nakai Y, Isayama H, Saito K, Hamada T, Takahara N, Mizuno S, Mohri D, Kogure H, Matsubara S, Yamamoto N, Tada M, Koike K.
  • Journal Title: Pancreas Volume: 46 Issue: 8 Pages: 1069-1075
  • DOI: 10.1097/mpa.0000000000000889
  • Peer Reviewed / Open Access
• [Journal Article] The Role of Pancreatic Enzyme Replacement Therapy in Unresectable Pancreatic Cancer: A Prospective CohortStudy (2017)
  • Author(s): Saito T, Hirano K, Isayama H, Nakai Y, Saito K, Umefune G, Akiyama D, Watanabe T, Takagi K, Hamada T, Takahara N, Uchino R, Mizuno S, Kogure H, Matsubara S, Yamamoto N, Tada M, Koike K.
  • Journal Title: Dig Endosc Volume: 46 Issue: 3 Pages: 341-346
  • DOI: 10.1097/mpa.0000000000000767
  • Peer Reviewed
• [Journal Article] The inhibition of renin-angiotensin system in advanced pancreatic cancer: an exploratory analysis in 349 patients (2015)
  • Author(s): Nakai Y, Isayama H, Sasaki T, Takahara N, Saito K, Ishigaki K, Hamada T, Mizuno S, Miyabayashi K, Yamamoto K, Mohri D, Kogure H, Yamamoto N, Ijichi H, Tateishi K, Tada M, Koike K.
  • Journal Title: J Cancer Res Clin Oncol. Volume: 144 Issue: 5 Pages: 933-939
  • DOI: 10.1007/s00432-014-1873-2
  • Peer Reviewed