• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Antitumor efficacy of oncolytic reovirus against gastrointestinal tumor

Research Project

Project/Area Number 15K09060
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionNagoya City University

Principal Investigator

Mori Yoshinori  名古屋市立大学, 大学院医学研究科, 研究員 (80468248)

Co-Investigator(Kenkyū-buntansha) 片岡 洋望  名古屋市立大学, 大学院医学研究科, 准教授 (40381785)
青山 峰芳  名古屋市立大学, 大学院薬学研究科, 教授 (70363918)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsレオウイルス / 腫瘍溶解性ウイルス / 消化管腫瘍 / 消化管間葉系腫瘍 / Fas / GIST / reovirus
Outline of Final Research Achievements

We investigated whether reovirus has antitumor activity against GIST cells in the setting of imatinib sensitivity. Cell proliferation and apoptosis assays were performed using a human GIST cell line, GIST-T1, and imatinib-resistant GIST (GIST-IR) cells that we established. The molecular pathways responsible for cell damage by reovirus were explored using PCR-arrays and Western blots. Reovirus significantly induced apoptotic cell death in GIST-T1 and GIST-IR cells in vitro, despite differences in the activation of receptor tyrosine kinase pathways between GIST-T1 and GIST-IR. Molecular assays indicated the possibility that reovirus induces apoptotic cell death via Fas signaling. Furthermore, in vivo mouse tumor xenograft models demonstrated a significant anti-tumor effect of reovirus on both GIST-T1 and GIST-IR cells. Our results demonstrate the therapeutic potential of reovirus against GIST.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2017 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results)

  • [Int'l Joint Research] University of Calgary/Dept Biochem & Molec Biol(カナダ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Anti-tumor efficacy of oncolytic reovirus against gastrointestinal stromal tumor cells.2017

    • Author(s)
      Inagaki Y, Kubota E, Mori Y, Aoyama M, Kataoka H, Johnston RN, Joh T.
    • Journal Title

      Oncotarget

      Volume: 8(70) Issue: 70 Pages: 115632-115646

    • DOI

      10.18632/oncotarget.23361

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 消化管Gastrointestinal stromal tumor (GIST)に対する腫瘍崩壊ウイルス・レオウイルスの抗腫瘍効果の検討2017

    • Author(s)
      稲垣 佑祐、久保田英嗣、市川紘、野尻優、野村智史、林則之、西江裕忠、片野敬仁、尾関啓司、岡本泰幸、溝下勤、志村貴也、森義徳、谷田諭、片岡洋望、城卓志
    • Organizer
      第13回日本消化管学会総会学術集会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2017-02-17
    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi