Development of novel cell transplantation method using cardiomyocytes derived from human iPS cells with microvessel structure

• Project/Area Number: 15K09098
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Keio University
• Principal Investigator: Kanazawa Hideaki 慶應義塾大学, 医学部(信濃町), 講師 (40338033)
• Co-Investigator(Renkei-kenkyūsha): FUJITA Jun 慶應義塾大学, 医学部, 特任准教授 (10306706) ; FUKUDA Keiichi 慶應義塾大学, 医学部, 教授 (20199227) ; FUKUDA Junji 横浜国立大学, 大学院工学研究院, 准教授 (80431675)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 心筋再生医療 / iPS由来心筋細胞

Outline of Final Research Achievements

By using a special incubator with an oxygen permeable poly-dimethylsiloxane (PDMS) membrane as the bottom surface, it was possible to product myocardial spheroids. These cardiomyocytes tended to have a high myosin light chain (MLY 2) which is a maturation marker. On the other hand, in the conventional incubator that does not permeate oxygen, the tendency of target gene expression of Hypoxia-Inducible Factor (HIF) was high.

Research Products

• [Journal Article] Efficient large-scale 2D culture system for human induced pluripotent stem cells and differentiated cardiomyocytes. (2017)
  • Author(s): Toyama S, Fujita J*, Fujita C, Yamaguchi M, Kanaami S, Ohno R, Sakamoto K, Kodama M, Kurokawa J, Kanazawa H, Seki T, Kishino Y, Okada M, Nakajima K, Tanosaki S, Someya S, Hirano A, Kawaguchi S, Kobayashi E, Fukuda K
  • Journal Title: Stem Cell Rep Volume: 9 Issue: 5 Pages: 1406-1414
  • DOI: 10.1016/j.stemcr.2017.08.025
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Manipulation of Pluripotent Stem Cell Metabolism for Clinical Application. (2017)
  • Author(s): Tohyama S, Tanosaki S, Someya S, Fujita J, Fukuda K.
  • Journal Title: Curr Stem Cell Rep. Volume: 3 Issue: 1 Pages: 28-34
  • DOI: 10.1007/s40778-017-0073-9
  • Peer Reviewed
• [Journal Article] Glutamine Oxidation Is Indispensable for Survival of Human Pluripotent Stem Cells. (2016)
  • Author(s): Tohyama S, Fujita J, Hishiki T, Matsuura T, Hattori F, Ohno R, Kanazawa H, Seki T, Nakajima K, Kishino Y, Okada M, Hirano A, Kuroda T, Yasuda S, Sato Y, Yuasa S, Sano M, Suematsu M, Fukuda K.
  • Journal Title: Cell Metab Volume: 23 Issue: 4 Pages: 663-74
  • DOI: 10.1016/j.cmet.2016.03.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Gelatin Hydrogel Enhances the Engraftment of Transplanted Cardiomyocytes and Angiogenesis to Ameliorate Cardiac Function after Myocardial Infarction. (2015)
  • Author(s): Nakajima K, Fujita J, Matsui M, Tohyama S, Tamura N, Kanazawa H, Seki T, Kishino Y, Hirano A, Okada M, Tabei R, Sano M, Goto S, Tabata Y, Fukuda K.
  • Journal Title: PLoS One Volume: 10(7) Issue: 7 Pages: e0133308-e0133308
  • DOI: 10.1371/journal.pone.0133308
  • NAID: 120005763407
  • Peer Reviewed / Open Access
• [Journal Article] Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions. (2015)
  • Author(s): Kishino Y, Seki T, Yuasa S, Fujita J, Fukuda K.
  • Journal Title: J Vis Exp. Volume: 105 Issue: 105 Pages: 105-105
  • DOI: 10.3791/53225
  • Peer Reviewed
• [Presentation] 臨床応用前夜となったiPS細胞による心筋再生医療の今後の展開 (2017)
  • Author(s): 福田恵一、遠山周吾、、関倫久、中嶋一晶、湯浅慎介、金澤英明、藤田淳
  • Organizer: 第21回日本心不全学会学術集会
• [Presentation] 臨床応用前夜となったiPS細胞による心筋再生医療の今後の展開 (2017)
  • Author(s): 福田恵一、遠山周吾、金澤英明、関倫久、中嶋一晶、藤田淳、湯浅慎介
  • Organizer: 第65回日本心臓病学会学術集会
• [Presentation] iPS由来心筋細胞を用いた重症心不全に対する心筋再生医療における移植デバイスの開発 (2016)
  • Author(s): 1.田部井亮太、金澤英明、藤田淳、遠山周吾、平野暁教、川口新治、志水秀行、田畑泰彦、小林英司、福田恵一
  • Organizer: 第20回日本適応医学会学術集会
  • Place of Presentation: 東京コンベンションホール
  • Year and Date: 2016-12-16
• [Presentation] Generation of NKX2-5 DsRed/w hiPSCs using TALE nucleases. (2015)
  • Author(s): 大野 麗、遠山 周吾, 藤田 淳, 福田 恵一
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-12-03
• [Book] Recent Improvements and Emerging Issues in iPSC Generation for the Modeling of Disease (2016)
  • Author(s): Seki T, Yuasa S, Fukuda K.
  • Publisher: Human iPS Cells in Disease Modelling