Phosphorylase phosphorylation in the heart failure development

• Project/Area Number: 15K09134
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Kanazawa University
• Principal Investigator: Konno Tetsuo 金沢大学, 医学系, 助教 (50377389)
• Co-Investigator(Kenkyū-buntansha): 林 研至 金沢大学, 附属病院, 助教 (00422642) ; 小田 彰史 名城大学, 薬学部, 教授 (50433511)
• Co-Investigator(Renkei-kenkyūsha): WAKIMOTO Hiroko ハーバード大学
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 心不全 / リン酸化 / ホスホリラーゼ / 肥大型心筋症

Outline of Final Research Achievements

We aimed to investigate the role of phosphorylase in the development of heart failure. In vitro forward assay showed that WT-phosphorylase activity was significantly decreased compared with ThrXAla phosphorylase and ThrXAsp phosphorylase. This association was also observed in the reverse gel assay.

Research Products

• [Journal Article] Late Gadolinium Enhancement for Prediction of Mutation-Positive Hypertrophic Cardiomyopathy on the Basis of Panel-Wide Sequencing (2018)
  • Author(s): Teramoto R, Fujino N, Konno T, Nomura A, Nagata Y, Tsuda T, Tada H, Sakata K, Yamagishi M, Hayashi K, Kawashiri MA.
  • Journal Title: Circulation Journal Volume: 82 Issue: 4 Pages: 1139-1148
  • DOI: 10.1253/circj.CJ-17-1012
  • NAID: 130006528636
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Journal Article] Characteristics of induced pluripotent stem cells from clinically divergent female monozygotic twins with Danon disease. (2018)
  • Author(s): Yoshida S, Nakanishi C, Okada H, Mori M, Yokawa J, Yoshimuta T, Ohta K, Konno T, Fujino N, Kawashiri MA, Yachie A, Yamagishi M, Hayashi K.
  • Journal Title: J Mol Cell Cardiol. Volume: 114 Pages: 234-242
  • DOI: 10.1016/j.yjmcc.2017.11.019
  • Peer Reviewed / Open Access
• [Journal Article] Heat Failure Phenotypes Induced by Knockdown of DAPIT in Zebrafish: A New Insight into Mechanism of Dilated Cardiomyopathy. (2017)
  • Author(s): Nagata Y, Yamagishi M, Konno T, Nakanishi C, Asano Y, Ito S, Nakajima Y, Seguchi O, Fujino N, Kawashiri MA, Takashima S, Kitakaze M, Hayashi K.
  • Journal Title: Sci Rep. Volume: 7 Issue: 1 Pages: 17417-17417
  • DOI: 10.1038/s41598-017-17572-y
  • Peer Reviewed / Open Access
• [Journal Article] Impact of T wave amplitude in lead aVR on predicting cardiac events in ischemic and nonischemic cardiomyopathy patients with an implantable cardioverter defibrillator. (2017)
  • Author(s): Tanaka Y, Konno T, Tamura Y, Tsuda T, Furusho H, Takamura M, Sakata K, Yamagishi M, Hayashi K.
  • Journal Title: Ann Noninvasive Electrocardiol. Volume: 22 Issue: 6 Pages: 12452-12452
  • DOI: 10.1111/anec.12452
  • Peer Reviewed
• [Journal Article] Usefulness of Electrocardiographic Voltage to Determine Myocardial Fibrosis in Hypertrophic Cardiomyopathy. (2016)
  • Author(s): Konno T, Nagata Y, Teramoto R, Fujino N, Nomura A, Tada H, Sakata K, Furusho H, Takamura M, Nakamura H, Kawashiri MA, Yamagishi M, Hayashi K.
  • Journal Title: Am J Cardiol. Volume: 117 Issue: 3 Pages: 443-449
  • DOI: 10.1016/j.amjcard.2015.11.015
  • Peer Reviewed / Open Access
• [Journal Article] Hypokalemia and the Disappearance of Giant Negative T Waves (2016)
  • Author(s): Konno T, Hayashi K, Fujino N, Yamagishi M
  • Journal Title: Internal Medicine Volume: 55 Issue: 5 Pages: 545-546
  • DOI: 10.2169/internalmedicine.55.5770
  • NAID: 130005132630
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access
• [Journal Article] Myocardial tissue characterization of left ventricular reverse remodeling in ischemic cardiomyopathy. (2016)
  • Author(s): Nagata Y, Konno T, Hayashi K, Kawashiri MA.
  • Journal Title: Circ J Volume: 80 Pages: 2427-2428
  • NAID: 130005285034
  • Peer Reviewed
• [Journal Article] Whole exome sequencing combined with integrated variant annotation prediction identifies a causative myosin essential light chain variant in hypertrophic cardiomyopathy. (2016)
  • Author(s): Nomura A, Tada H, Teramoto R, Konno T, Hodatsu A, Won HH, Kathiresan S, Ino H, Fujino N, Yamagishi M, Hayashi K.
  • Journal Title: J Cardiol. Volume: 2 Issue: 2 Pages: 133-9
  • DOI: 10.1016/j.jjcc.2015.09.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Electrocardiographic QRS Fragmentation as a Marker for Myocardial Fibrosis in Hypertrophic Cardiomyopathy. (2015)
  • Author(s): Konno T, Hayashi K, Fujino N, Oka R, Nomura A, Nagata Y, Hodatsu A, Sakata K, Furusho H, Takamura M, Nakamura H, Kawashiri MA, Yamagishi M.
  • Journal Title: J Cardiovasc Electrophysiol. Volume: 26 Issue: 10 Pages: 1081-1087
  • DOI: 10.1111/jce.12742
  • Peer Reviewed / Open Access
• [Journal Article] Right ventricular hypertrophy is associated with cardiovascular events in hypertrophic cardiomyopathy: evidence from study with magnetic resonance imaging. (2015)
  • Author(s): Nagata Y, Konno T, Fujino N, Hodatsu A, Nomura A, Hayashi K, Nakamura H, Kawashiri MA, Yamagishi M.
  • Journal Title: Can J Cardiol. Volume: 31 Issue: 6 Pages: 702-708
  • DOI: 10.1016/j.cjca.2014.12.036
  • Peer Reviewed / Open Access
• [Journal Article] Increased extent of myocardial fibrosis in genotyped hypertrophic cardiomyopathy with ventricular tachyarrhythmias. (2014)
  • Author(s): Fujita T, Konno T, Yokawa J, Masuta E, Nagata Y, Fujino N, Funada A, Hodatsu A, Kawashiri MA, Yamagishi M, Hayashi K.
  • Journal Title: J Cardiol. Volume: in press Issue: 1 Pages: 63-68
  • DOI: 10.1016/j.jjcc.2014.10.002
  • Peer Reviewed / Open Access
• [Journal Article] Fragmented QRS Predicts Heart Failure Progression in Patients With Hypertrophic Cardiomyopathy (2014)
  • Author(s): Nomura A, Konno T, Fujita T, Tanaka Y, Nagata Y, Tsuda T, Hodatsu A, Sakata K, Nakamura H, Kawashiri MA, Fujino N, Yamagishi M, Hayashi K.
  • Journal Title: Circulation Journal Volume: 79 Issue: 1 Pages: 136-143
  • DOI: 10.1253/circj.CJ-14-0822
  • NAID: 130004704014
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Presentation] 肥大型心筋症における心筋線維化：遺伝子型および遺伝子発現の観点から (2017)
  • Author(s): 今野哲雄
  • Organizer: 第81回日本循環器学会学術集会
  • Place of Presentation: 金沢
  • Invited
• [Presentation] Impact of genotype on conduction disturbance in patients with hypertrophic cardiomyopathy (2017)
  • Author(s): 今野哲雄
  • Organizer: 第81回日本循環器学会学術集会
  • Place of Presentation: 金沢
• [Presentation] Low Electrocardiographic Voltage Reflects Increased Myocardial Fibrosis in Hypertrophic Cardiomyopathy (2016)
  • Author(s): Konno T, Nagata Y, Teramoto R, Fujino N, Nomura A, Tada H, Sakata K, Hayashi K, Yamagishi M.
  • Organizer: 日本循環器学会学術集会総会 2016
  • Place of Presentation: 仙台、宮城
  • Year and Date: 2016-03-18
• [Presentation] Inclusion of Accessory Right Precordial Leads Improves Predictive Ability of Butler Voltage Index for Right Ventricular Hypertrophy in Hypertrophic Cardiomyopathy (2016)
  • Author(s): Konno T, Nagata Y, Teramoto R, Fujino N, Nomura A, Sakata K, Hayashi K, Yamagishi M.
  • Organizer: 日本循環器学会学術集会総会 2016
  • Place of Presentation: 仙台、宮城
  • Year and Date: 2016-03-18
• [Presentation] 心筋症の遺伝子診断 (2016)
  • Author(s): 今野哲雄
  • Organizer: 第20回日本心不全学会学術集会
  • Place of Presentation: 札幌
  • Invited
• [Presentation] Impact of left ventricular wall hypertrophy relative to right ventricular wall thickness on pathological remodeling and adverse cardiac events in hypertrophic cardiomyopathy (2015)
  • Author(s): Konno T, Nagata Y, Teramoto R, Masuta E, Hayashi K, Fujino N, Yamagishi M.
  • Organizer: European Society of Cardiology, Annual congress in 2015
  • Place of Presentation: London, UK
  • Year and Date: 2015-08-29
  • Int'l Joint Research
• [Presentation] Fragmented QRS improves prediction ability of the Risk-SCD calculator for sudden cardiac death in Japanese patients with hypertrophic cardiomyopathy. (2015)
  • Author(s): Teramoto R, Konno T, Tada H, Tsuda T, Hodatsu A, Sakata K, Hayashi K, Fujino N, Kawashiri MA, Yamagishi M.
  • Organizer: European Society of Cardiology, Annual congress in 2015
  • Place of Presentation: London, UK
  • Year and Date: 2015-08-29
  • Int'l Joint Research
• [Book] 循環器内科 第77巻4号 (2015)
  • Author(s): 寺本了太、今野哲雄、宝達明彦、藤野陽、林研至、山岸正和
  • Total Pages: 4
  • Publisher: 科学評論社 東京
• [Remarks] 金沢大学循環器病態内科学教室
  • URL: http://intmed2.w3.kanazawa-u.ac.jp/
• [Remarks] 金沢大学大学院医薬保健総合研究科 循環器病態内科学
  • URL: http://intmed2.w3.kanazawa-u.ac.jp/circulatory/