| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
To understand the molecular mechanisms of Polycomb group (PcG) proteins in maintaining the cardiac functions, we analyzed Pcgf5 (PcG ring finger 5) functions at adult stage. As reported previously, Pcgf5 formed PRC1.5 complex with Ring1b and RYBP in adult mouse left ventricle (LV). We also found casein kinase 2 alpha 1 (CK2α1) and histone deacetylase 2 (HDAC2) associated with Pcgf5 and other PRC1.5 components. In several phosphoproteins, significant alterations were observed in young and aged Pcgf5-deficient LV. We also compared gene expressions in young and aged LV by RNA-seq analysis and defined specific expression changes in aged Pcgf5-deleted LV. These findings revealed that Pcgf5 plays a pivotal role in maintaining the cardiac function in aged mice.
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