Cell culture and diagnosis of lung cancer using bronchial brush specimens

• Project/Area Number: 15K09204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Hokkaido University
• Principal Investigator: Kikuchi Eiki 北海道大学, 大学病院, 助教 (60463741)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 細胞培養 / 肺癌

Outline of Final Research Achievements

Cell culture was performed using a total of 80 specimens of 77 cases of bronchial brushing specimen, 2 cases of pleural effusion and 1 autopsy lung, and cell culture and passage were possible in 53 of them (66.3%). Most of the cells that could not be cultured were due to bacterial contamination, probably by bacteria from lower respiratory tract. The EGFR and human KRAS gene sequence showed that all of the 53 cultured cells have human genes, and the KRAS mutation was observed in one of 8 cases.