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The analyses of cell type-specific extracellular vesicles derived from lung quiescent or cytokine-activated lung endothelial cells

Research Project

Project/Area Number 15K09206
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionTohoku University

Principal Investigator

YAMADA MITSUHIRO  東北大学, 大学病院, 助教 (00396483)

Co-Investigator(Kenkyū-buntansha) 吉岡 祐亮 (吉岡祐亮)  国立研究開発法人国立がん研究センター, 研究所, 研究員 (60721503)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsバイオマーカー / COPD / IPF / エクソソーム / 細胞外小胞 / ARDS / マイクロパーティクル
Outline of Final Research Achievements

Serum EV miR-21-5p was elevated in both the acute inflammatory phase (day 7) and the chronic fibrotic phase (day 28) in the mouse model. In the clinical setting, serum EV miR-21-5p was significantly higher in IPF patients than in healthy control subjects. The baseline serum EV miR-21-5p was correlated with the rate of decline in vital capacity over 6 months. Furthermore, serum EV miR-21-5p was independently associated with mortality during the following 30 months, even after adjustment for other variables. In the survival analysis, IPF patients whose baseline serum EV miR-21-5p was high had a significantly poorer prognosis over 30 months. Our results suggest that serum EV miR-21-5p has potential as a prognostic biomarker for IPF.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (22 results)

All 2018 2017 2016 2015

All Journal Article (12 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results) Book (1 results)

  • [Journal Article] The cholinergic anti-inflammatory pathway: an innovative treatment strategy for respiratory diseases and their comorbidities2018

    • Author(s)
      Yamada Mitsuhiro、Ichinose Masakazu
    • Journal Title

      Current opinion in pharmacology

      Volume: 40 Pages: 18-25

    • DOI

      10.1016/j.coph.2017.12.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ARDSにおけるエクソソームとmiRNA2018

    • Author(s)
      山田 充啓
    • Journal Title

      呼吸器内科

      Volume: 33 Pages: 66-71

    • NAID

      40021450795

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 【先制医療-予防医療の最前線-】 先制医療・予防医療の実際 感染性疾患(ワクチン接種)2018

    • Author(s)
      山田 充啓, 渡辺 彰
    • Journal Title

      診断と治療

      Volume: 106 Pages: 49-55

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Invasive pulmonary mucormycosis: rare presentation with pulmonary eosinophilia.2017

    • Author(s)
      Hirano T, Yamada M, Sato K, Murakami K, Tamai T, Mitsuhashi Y, Tamada T, Sugiura H, Sato N, Saito R, Tominaga J, Watanabe A, Ichinose M.
    • Journal Title

      BMC Pulm Med

      Volume: 17 Issue: 1 Pages: 76-76

    • DOI

      10.1186/s12890-017-0419-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 医学と医療の最前線 肺炎診療の動向(解説)2017

    • Author(s)
      山田 充啓
    • Journal Title

      日本内科学会雑誌

      Volume: 106 Pages: 2246-2253

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 炎症性呼吸器疾患バイオマーカーとしてのエクソソームの意義2017

    • Author(s)
      山田充啓
    • Journal Title

      医学のあゆみ

      Volume: 260 Pages: 533-534

    • Related Report
      2016 Research-status Report
  • [Journal Article] Serum extracellular vesicular miR-21-5p is a predictor of the prognosis in idiopathic pulmonary fibrosis.2016

    • Author(s)
      Makiguchi T, Yamada M, Yoshioka Y, Sugiura H, Koarai A, Chiba S, Fujino N, Tojo Y, Ota C, Kubo H, Kobayashi S, Yanai M, Shimura S, Ochiya T, Ichinose M.
    • Journal Title

      Respir Res

      Volume: 17 Issue: 1 Pages: 110-110

    • DOI

      10.1186/s12931-016-0427-3

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Inflammatory responses in the initiation of lung repair and regeneration: their role in stimulating lung resident stem cells2016

    • Author(s)
      Mitsuhiro Yamada, Naoya Fujino, Masakazu Ichinose
    • Journal Title

      Inflammation and Regeneration

      Volume: 36 Issue: 1 Pages: 15-15

    • DOI

      10.1186/s41232-016-0020-7

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cutting edge of COPD therapy: current pharmacological therapy and future direction.2015

    • Author(s)
      Yamada Mitsuhiro and Ichinose Masakazu
    • Journal Title

      COPD Research and Practice

      Volume: 1 Pages: 5-5

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 呼吸器疾患の分子病態に迫る「エクソソーム」2015

    • Author(s)
      山田充啓
    • Journal Title

      Respiratory Medical Research

      Volume: 3 Pages: 8-13

    • Related Report
      2015 Research-status Report
  • [Journal Article] エクソソームが映し出す血管病の新たな側面 「血管炎症におけるエクソソームを用いたバイオマーカー」2015

    • Author(s)
      山田充啓
    • Journal Title

      血管医学

      Volume: 16 Pages: 61-68

    • Related Report
      2015 Research-status Report
  • [Journal Article] 細胞外小胞によるバイオマーカーの進展「細胞外小胞に着目した呼吸器疾患の診断・病態マーカーの探索」2015

    • Author(s)
      山田充啓
    • Journal Title

      医学のあゆみ

      Volume: 255 Pages: 203-208

    • Related Report
      2015 Research-status Report
  • [Presentation] Gene Expression Profiles in Isolated Lung Endothelial Cells in Chronic Obstructive Pulmonary Disease.2017

    • Author(s)
      18.Tojo Y, Yamada M*, Fujino N, Chiba S, Shibuya R, Koarai A, Sugiura H, Ogawa H,. Ichinose M.
    • Organizer
      The International Conference of the American Thoracic Society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 重症喘息の定義・分類と治療戦略 ―専門医の腕が試される― 「重症喘息の治療戦略:下気道感染の観点から」2017

    • Author(s)
      山田充啓
    • Organizer
      第66回日本アレルギー学会学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Identification of serum extracellular vesicle microRNA as a biomarker for prognosis in idiopathic pulmonary fibrosis2016

    • Author(s)
      Tomonori Makiguchi , Mitsuhiro Yamada , Yusuke Yoshioka , Hisatoshi Sugiura , Akira Koarai , Hiroshi Kubo , Takahiro Ochiya , Masakazu Ichinose
    • Organizer
      ATS 2016
    • Place of Presentation
      サンフランシスコ、米国
    • Year and Date
      2016-05-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 特発性肺線維症の予後予測因子としての血清エクソソームmicroRNA2016

    • Author(s)
      牧口友紀、山田充啓、吉岡祐亮、杉浦久敏、小荒井晃、大田千晴、久保裕司、小林誠一、矢内勝、志村早苗、落谷孝広、一ノ瀬正和
    • Organizer
      第56回 日本呼吸器学会学術講演会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2016-04-10
    • Related Report
      2016 Research-status Report
  • [Presentation] microRNAと細胞外小胞:病態での役割とバイオマーカーとしての意義2016

    • Author(s)
      山田充啓
    • Organizer
      第56回 日本呼吸器学会学術講演会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2016-04-09
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 炎症は再生を抑制するか“Inflammation and Regeneration in Cross-organs”「肺の再生と炎症」2015

    • Author(s)
      山田充啓
    • Organizer
      第36回 日本炎症・再生医学会 シンポジウム
    • Place of Presentation
      東京、虎ノ門ヒルズフォーラム
    • Year and Date
      2015-07-22
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] The Detection of Cell Type-Specific Extracellular Vesicles Derived from Lung Epithelial Cells, Stable or Cytokine-Activated Endothelial Cells by Exoscreen.2015

    • Author(s)
      Makiguchi T, Yamada M, Yoshioka Y, Sugiura H, Koarai A, Kubo H, Ochiya T, Kikuchi T, Ichinose M.
    • Organizer
      American Thoracic Society International Conference
    • Place of Presentation
      Denver, USA
    • Year and Date
      2015-05-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 炎症刺激・酸化・ニトロ化ストレス下の血管内皮細胞由来エクソソームの動態2015

    • Author(s)
      牧口友紀,山田充啓,吉岡祐亮,杉浦久敏,小荒井晃,久保裕司,落谷孝広,一ノ瀬正和
    • Organizer
      第55回日本呼吸器学会学術講演会
    • Place of Presentation
      東京、東京国際フォーラム
    • Year and Date
      2015-04-18
    • Related Report
      2015 Research-status Report
  • [Presentation] RDSの多面的評価 「エクソソームとmicroRNA: 新規病態マーカーとしての可能性」2015

    • Author(s)
      山田充啓
    • Organizer
      第55回日本呼吸器学会学術講演会 シンポジウム
    • Place of Presentation
      東京、東京国際フォーラム
    • Year and Date
      2015-04-17
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] パラダイムシフトをもたらすエクソソーム機能研究最前線:エクソソームを用いた炎症性肺疾患新規 バイオマーカーの探索2017

    • Author(s)
      山田充啓
    • Total Pages
      9
    • Publisher
      株式会社エヌ・ティー・エス
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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