The involvement of proton-sensing receptor OGR1 in chronic airway inflammation and secretion.
| Project/Area Number |
15K09213
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | University of Fukui |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
OKAJIMA Fumikazu 青森大学, 薬学部, 教授 (30142748)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | プロトン / OGR1 / 気道炎症 / IL-8 / 気管支平滑筋細胞 / グルココルチコイド / ヒト気管支平滑筋細胞 / NF-kB / AP-1 / ステロイド / ＮＦ－ｋＢ / ＡＰ－１ / ＩＬ－８ / 遺伝子転写 / デキサメタゾン |
|---|
| Outline of Final Research Achievements |
Human bronchial smooth muscle cells (BSMCs) are involved in airway inflammatio mation. Ovarian cancer G protein-coupled receptor 1 (OGR1) sense extracellular protons and mediate cytokine production in BSMCs. BSMCs were stimulated by pH 6.3 or pH 7.4-adjusted DMEM containing 0.1% BSA (0.1% BSA-DMEM). As a control stimulant, TNF-alpha (10 ng/ml) was used. IL-8 mRNA expression in BSMCs was quantified by RT-PCR. Acidic pH induced mRNA expression of IL-8 and a substantial amount of IL-8 production in BSMCs. BSMCs transfected by siRNA specific for OGR1 produced less IL-8 compared with those transfected non-targeting siRNA. PKC inhibitor, MEK 1/2 inhibitor, and the inhibitor of IkB phosphorylation reduced acidic pH-stimulated IL-8 production in BSMCs. Dexamethasone inhibited acidic pH-stimulated IL-8 production of BSMCs in a dose-dependent manner. Dexamethasone did not affect either phosphorylation or binding to consensus DNA site of NF-kB p65.
|
Report
(4 results)
Research Products
(15 results)
-
-
-
-
[Journal Article] Nattokinase, profibrinolytic enzyme, effectively shrinks the nasal polyp tissue and decreases viscosity of mucus2017
Author(s)
Takabayashi T, Imoto Y, Sakashita M, Kato Y, Tokunaga T, Yoshida K, Narita N, Ishizuka T, Fujieda S.
-
Journal Title
Allergology International
Volume: 66
Issue: 4
Pages: 594-602
DOI
NAID
ISSN
1323-8930, 1440-1592
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
