| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
COPD and IPF are chronically progressive and lethal lung diseases. Extracellular vesicles (EVs), including exosome and microvesicle, are recognized as important mediators of intercellular communication. We investigated the involvement of EV-mediated intercellular communication between lung fibroblasts (LFs) and human bronchial epithelial cells (HBECs) in COPD and IPF pathogenesis. We confirmed that they were internalized when incubated with HBECs or fibroblasts.Cigarette smoking extracts (CSE) induced cellular senescence in HBEC. EVs derived from senescent HBEC induced myofibroblast differentiation. We identified miR210 in exosome deribed from senescent HBEC as an effector miRNA. EVs derived from IPF-LFs induced mitochondrial dysfunction and ROS accompanied by cellular senescence in HBECs. Three upregulated EV miRNAs from IPF-LF regulated cellular senescence and mitochondrial dysfunction via targeting SIRT3 in HBEC.
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