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The role of MafB in CKD.

Research Project

Project/Area Number 15K09241
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Kidney internal medicine
Research InstitutionUniversity of Tsukuba

Principal Investigator

Morito Naoki  筑波大学, 医学医療系, 講師 (70463825)

Research Collaborator USUI TOSHIAKI  筑波大学, 医学医療系, 講師
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords糸球体上皮細胞 / 転写因子 / 巣状糸球体硬化症
Outline of Final Research Achievements

Analysis of MafB deficient (MafB -/-) mice revealed that MafB is essential for podocyte differentiation and its foot process formation. However, the role of MafB in adult kidneys was not well known. Because MafB -/- mice die during the perinatal period, we used MafB heterozygote (MafB +/-) mice in this study.
We found that MafB +/- mice developed overt albuminuria at 50 week-old of age. By the electron microscopy analysis, we observed podocyte foot process effacement in MafB +/- mice. RT-PCR analysis revealed the Nephrin and Podocin glomerular expressions were decreased in MafB +/- mice compared to wild-type animals. We exploited transgenic mice expressing MafB in podocytes using Nephrin promoter (NPHS1 -MafB TG mice). We found that the NPHS1 MafB transgene successfully restored MafB mice to normal albuminuria at 50 week-old of age.
Conclusions: MafB is essential for the maintenance of podocytes. MafB could be a therapeutic target in chronic kidney disease.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 2016 2015

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Transcription factor MafB may play an important role in secondary hyperparathyroidism.2018

    • Author(s)
      Morito N, Yoh K, Usui T, Oishi H, Ojima M, Fujita A, Koshida R, Shawki HH, Hamada M, Muratani M, Yamagata K, Takahashi S.
    • Journal Title

      Kidney International

      Volume: 93 Issue: 1 Pages: 54-68

    • DOI

      10.1016/j.kint.2017.06.023

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] MAFB IS ESSENTIAL FOR THE MAINTENANCE OF PODOCYTES IN BOTH MICE AND HUMANS.2017

    • Author(s)
      臼井俊明、森戸直記
    • Organizer
      第54回ヨーロッパ腎臓学会 マドリード(スペイン)2017年6月3-6日
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 転写因子Mafbによる副甲状腺ホルモンの制御2016

    • Author(s)
      森戸 直記
    • Organizer
      第59回日本腎臓学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-06-17
    • Related Report
      2016 Research-status Report
  • [Presentation] MAFB MUTANT SHOWS PODOCYTE INJURY WITH AGING2016

    • Author(s)
      Naoki Morito
    • Organizer
      第53回ヨーロッパ腎臓学会
    • Place of Presentation
      Messe Wien Exhibition & Congress Centre(ウィーン、オーストリア)
    • Year and Date
      2016-05-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 転写因子Mafbのハプロ不全はpodocyte障害をもたらす2015

    • Author(s)
      森戸直記
    • Organizer
      日本腎臓学会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2015-06-05
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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