| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we have succeeded in inducing human iPS cells into anterior intermediate mesoderm cells and Wolffian duct (nephric duct) cells at the induction rate more than 70% in two-dimensional adherent cultures and established the method to generate ureteric bud (UB)-like tissues in three-dimensional cultures using gels from the induced Wolffian duct cells. The generated UB-like tissues exhibited their typical biological features, budding and branching, and the expression of marker genes, such as GATA3, RET, CK19, PAX2 and CALB1. Furthermore, we have succeeded in generating the UB-like tissues from iPS cells derived from the patients with autosomal recessive polycystic kidney disease (ARPKD) by applying the established differentiation method. We are currently examining the disease phenotypes in the UB-like tissues by comparing their counterparts generated from control iPS cells and developing the differentiation method to induce collecting ducts from UB-like tissues.
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