• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

ROCK2 regulation of podocyte function in diabetic kidney disease

Research Project

Project/Area Number 15K09277
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Kidney internal medicine
Research InstitutionJikei University School of Medicine

Principal Investigator

Utsunomiya Kazunori  東京慈恵会医科大学, 医学部, 教授 (50185047)

Co-Investigator(Kenkyū-buntansha) 川浪 大治  東京慈恵会医科大学, 医学部, 准教授 (50568889)
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords糖尿病腎症 / 糸球体上皮細胞 / ポドサイト / Rho-kinase / ROCK2
Outline of Final Research Achievements

Diabetic kidney disease (DKD) remains leading cause of end stage renal disease and therefore a major burden on the healthcare system. Activation of ROCK pathway leads to the phosphorylation of downstream substrates, including myosin phosphatase target subunit, which has been postulated to control a variety of cellular functions such as cell proliferation, contraction, migration, and transcriptional regulation. A series of recent animal model studies have implicated ROCK signaling in cardiovascular and renal disease. In the present study, we identified ROCK2 as an important regulator of glomerular podocyte biability using cultured cells. In order to elucidate the role of ROCK in vivo, we generated podocyte-specific ROCK2 deletion mice. Gene expression and immunohistochemical studies will be performed in the mice to fully determine the significance of ROCK2 in the process of DKD.

Academic Significance and Societal Importance of the Research Achievements

ROCKは低分子量GTP結合タンパク質Rhoの標的タンパク質として同定されたセリン・スレオニンキナーゼである。これまでの国内外における研究により、ROCKは細胞収縮や増殖、遊走、遺伝子発現誘導等、細胞の重要な生理機能に関与することが示されている。また各種疾患モデル動物を用いた研究によってROCKの活性亢進が様々な病態を悪化させる原因となっていることが明らかになり、創薬のターゲットとして注目されている。本研究によってROCK2が糖尿病腎症の進展で重要な因子であると明らかになれば、今後これを標的とした有効な治療薬開発へつながる可能性がある。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2019 2018 2017 2016

All Journal Article (13 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 12 results,  Open Access: 8 results,  Acknowledgement Compliant: 3 results) Presentation (3 results)

  • [Journal Article] ROCK2 Regulates Monocyte Migration and Cell to Cell Adhesion in Vascular Endothelial Cells.2019

    • Author(s)
      Takeda Y, Matoba K, Kawanami D, Nagai Y, Akamine T, Ishizawa S, Kanazawa Y, Yokota T, Utsunomiya K
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20 Issue: 6 Pages: 1331-1331

    • DOI

      10.3390/ijms20061331

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Establishment of a myelinating co-culture system with a motor neuron-like cell line NSC-34 and an adult rat Schwann cell line IFRS1.2018

    • Author(s)
      Takaku S, Yako H, Niimi N, Akamine T, Kawanami D, Utsunomiya K, Sango K.
    • Journal Title

      Histochem Cell Biol

      Volume: 印刷中 Issue: 5 Pages: 537-543

    • DOI

      10.1007/s00418-018-1649-x

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Effect of Novel Glucose Monitoring System (Flash Glucose Monitoring) on Mental Well-being and Treatment Satisfaction in Japanese People with Diabetes.2018

    • Author(s)
      Mitsuishi S, Nishimura R, Harashima SI, Kawamura T, Tsujino D, Koide K, Nishimura A, Utsunomiya K, Inagaki N, Atsumi Y.
    • Journal Title

      Adv Ther

      Volume: 35 Issue: 1 Pages: 72-80

    • DOI

      10.1007/s12325-017-0649-x

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Primary Aldosteronism With Type 2 Diabetes Mellitus Requires More Antihypertensive Drugs for Blood Pressure Control: A Retrospective Observational Study.2018

    • Author(s)
      Ohashi K, Hayashi T, Watanabe Y, Hara K, Ukichi R, Asano H, Suzuki H, Yamashiro K, Tojo K, Sakamoto M, Utsunomiya K.
    • Journal Title

      J Clin Med Res

      Volume: 10 Issue: 1 Pages: 56-62

    • DOI

      10.14740/jocmr3233w

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lactic-fermented egg white improves visceral fat obesity in Japanese subjects-double-blind, placebo-controlled study.2017

    • Author(s)
      Matsuoka R, Kamachi K, Usuda M, Wang W, Masuda Y, Kunou M, Tanaka A, Utsunomiya K
    • Journal Title

      Lipids Health Dis

      Volume: 16 Issue: 1 Pages: 237-237

    • DOI

      10.1186/s12944-017-0631-2

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] High Glucose Stimulates Mineralocorticoid Receptor Transcriptional Activity Through the Protein Kinase C β Signaling.2017

    • Author(s)
      Hayashi T, Shibata H, Kurihara I, Yokota K, Mitsuishi Y, Ohashi K, Murai-Takeda A, Jo R, Ohyama T, Sakamoto M, Tojo K, Tajima N, Utsunomiya K, Itoh H.
    • Journal Title

      Int Heart J

      Volume: 58 Pages: 794-802

    • NAID

      130006179200

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Time-dependent effects of ipragliflozin on behaviour and energy homeostasis in normal and type 2 diabetic rats: continuous glucose telemetry analysis.2017

    • Author(s)
      Iuchi H, Sakamoto M, Matsutani D, Suzuki H, Kayama Y, Takeda N, Minamisawa S, Utsunomiya K.
    • Journal Title

      Sci Rep

      Volume: 22 Issue: 1 Pages: 351-354

    • DOI

      10.1038/s41598-017-12106-y

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rho-Kinase Blockade Attenuates Podocyte Apoptosis by Inhibiting the Notch Signaling Pathway in Diabetic Nephropathy.2017

    • Author(s)
      Matoba K, Kawanami D, Nagai Y, Takeda Y, Akamine T, Ishizawa S, Kanazawa Y, Yokota T, Utsunomiya K.
    • Journal Title

      Int J Mol Sci

      Volume: 18 Issue: 8 Pages: 1795-1795

    • DOI

      10.3390/ijms18081795

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] SGLT2 Inhibitors as a Therapeutic Option for Diabetic Nephropathy.2017

    • Author(s)
      Kawanami D, Matoba K, Takeda Y, Nagai Y, Akamine T, Yokota T, Sango K, Utsunomiya K.
    • Journal Title

      Int J Mol Sci

      Volume: 18 Issue: 5 Pages: 1083-1083

    • DOI

      10.3390/ijms18051083

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Incretin-based Therapies for Diabetic Complications: Basic Mechanisms and Clinical Evidence.2016

    • Author(s)
      Daiji Kawanami, Keiichiro Matoba, Kazunori Sango, Kazunori Utsunomiya
    • Journal Title

      International Journal of Molecular Medicine

      Volume: 17 Issue: 8 Pages: 1223-1223

    • DOI

      10.3390/ijms17081223

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Signaling pathways in diabetic nephropathy2016

    • Author(s)
      Daiji Kawanami, Keiichiro Matoba, Kazunori Utsunomiya
    • Journal Title

      Histology and Histopathology

      Volume: 31 Pages: 1059-67

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dyslipidemia in diabetic nephropathy2016

    • Author(s)
      Kawanami D, Matoba K, Utsunomiya K
    • Journal Title

      Renal Replacement Therapy

      Volume: 未定

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Rho/Rho-kinase2016

    • Author(s)
      川浪大治、宇都宮一典
    • Journal Title

      日本臨牀

      Volume: 74 Pages: 77-83

    • Related Report
      2015 Research-status Report
  • [Presentation] ROCK2は血管内皮における接着分子の発現と単球接着を制御する2018

    • Author(s)
      竹田 裕介, 的場 圭一郎, 川浪 大治, 永井 洋介, 赤嶺 友代, 石澤 将, 金澤 康, 横田 太持, 宇都宮 一典
    • Organizer
      第61回日本糖尿病学会年次学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Rho-kinaseはメサンギウム細胞においてアクチン動態を介して糸球体硬化を制御する2018

    • Author(s)
      永井 洋介, 川浪 大治, 的場 圭一郎, 竹田 裕介, 赤嶺 友代, 石澤 将, 金澤 康, 横田 太持, 宇都宮 一典
    • Organizer
      第61回日本糖尿病学会年次学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 糖尿病腎症の病態解明cutting edge 糖尿病腎症の発症・進展におけるRho-kinaseの意義2018

    • Author(s)
      川浪 大治, 的場 圭一郎, 永井 洋介, 竹田 裕介, 赤嶺 友代, 石澤 将, 金澤 康, 横田 太持, 宇都宮 一典
    • Organizer
      第61回日本糖尿病学会年次学術集会
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi