| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The analysis of intracellular trafficking pathway of albumin through glomerular endothelial cells after endocytosis via caveolae has been performed.
Co-localization of immunofluorescence study of albumin with several organelle and cytoskeletons showed transcytosis that albumin moved not along with actins and microtubules, reached to early endosome, and sorted to bypass endoplasmic reticulum, golgi apparatus, and lysosomes. The experiment to use transwells plates showed that the exocytosis of albumin to the other side of cells.
In the in vivo study with nephrotic syndrome modeled mouse induced by the peritoneal injection of puromycin, the inhibitors of caveolae reduced the amount of albuminuria.All these results indicated that intracellular trafficking pathway of albumin through glomerular endotherial cells after endocytosis via caveolae as the new etiology of urinary albumin excretion.
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