• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of molecular mechanisms for AQP2 activation to deveplop its inhibitor

Research Project

Project/Area Number 15K09286
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Kidney internal medicine
Research InstitutionTokyo Medical and Dental University

Principal Investigator

YUI Naofumi  東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (00633976)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords水チャネル / リン酸化 / 脱リン酸化 / 細胞内輸送 / 集合管 / バソプレシン / アクアポリン2 / 尿崩症 / アクアポリン2 / 心不全
Outline of Final Research Achievements

In this study, we developed a phospho-specific AQP2 immuno-isolation technique, and identified that vasopressin-mediated AQP2 Ser-261 phospho-regulation is processing in combination with Ser-256 and Ser-269 phosphorylation. pS256-pS261-AQP2 is phophorylated at Ser-269 to be pS256-pS261-pS269 triplly phospho-form, and then dephosphorylated at Ser-261 to be transformed into pS256-pS269-AQP2. We further revealed that the subsequent Ser-261 dephosphorylation was required to be accumulated in apical plasma membrane in concert with prior Ser-256 and Ser-269 phosphorylation.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (23 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 6 results,  Acknowledgement Compliant: 4 results) Presentation (11 results) (of which Int'l Joint Research: 5 results) Remarks (2 results)

  • [Int'l Joint Research] Massachusetts General Hospital(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] AKAPs-PKA disruptors increase AQP2 activity independently of vasopressin in a model of nephrogenic diabetes insipidus2018

    • Author(s)
      Ando Fumiaki、Mori Shuichi、Yui Naofumi、Morimoto Tetsuji、Nomura Naohiro、Sohara Eisei、Rai Tatemitsu、Sasaki Sei、Kondo Yoshiaki、Kagechika Hiroyuki、Uchida Shinichi
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 1411-1411

    • DOI

      10.1038/s41467-018-03771-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The proteasome inhibitor bortezomib attenuates renalfibrosis in mice via supression of TGF b12018

    • Author(s)
      Zeniya M, Mori T, Yui N, Nomura N, Mandai S, Isobe K, Chiga M, Sohara E, Rai T, Uchida S.
    • Journal Title

      Scientific Reports

      Volume: 7 Pages: 13086-13086

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ser-261 phospho-regulation is involved in pS256 and pS269-mediated apical translocation.2017

    • Author(s)
      Yui N, Ando F, Sasaki S, Uchida S.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 490 Pages: 1039-1039

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ezrin directly interacts with AQP2 and promotes its endocytosis.2017

    • Author(s)
      Li W, Jin WW, Tsuji K, Chen Y, Nomura N, Su L, Yui N, Arthur J, Cotecchia S, Paunescu TG, Brown D, Lu HAJ.
    • Journal Title

      Journal of Cell Science

      Volume: 130 Pages: 2914-2914

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation2017

    • Author(s)
      Naofumi Yui, Sei Sasaki, Shinichi Uchida
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 482 Issue: 4 Pages: 524-529

    • DOI

      10.1016/j.bbrc.2016.11.118

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Wnt5a induces renal AQP2 expression by activating calcineurin signalling pathway.2016

    • Author(s)
      Ando F, Sohara E, Morimoto T, Yui N, Nomura N, Kikuchi E, Takahashi D, Mori T, Vandewalle A, Rai T, Sasaki S, Kondo Y, Uchida S.
    • Journal Title

      Nat Commun.

      Volume: 7 Issue: 1 Pages: 13636-13636

    • DOI

      10.1038/ncomms13636

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Bence-Jones Protein λ-type Multiple Myeloma Patient Withdrawn from Maintenance Hemodialysis after Long-term Bortezomib and Dexamethasone Therapy2016

    • Author(s)
      Wakaba Yamaguchi, Naofumi Yui, Toshikage Nagao, Gaku Oshikawa, Mariko Negi, Soichiro Iimori, Tomokazu Okado, Tatemitsu Rai, Shinichi Uchida.
    • Journal Title

      Internal Medicine

      Volume: 55 Issue: 3 Pages: 263-268

    • DOI

      10.2169/internalmedicine.55.5310

    • NAID

      130005122595

    • ISSN
      0918-2918, 1349-7235
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Retroperitoneal Fibrosis in Chronic Kidney Disease2015

    • Author(s)
      Yuki Yoshizaki, Naofumi Yui, Tomokazu Okado, Junichi Ishigami, Soichiro Iimori, Katsuyuki Oi, Eisei Sohara, Sei Sasaki, Tatemitsu Rai, Shinichi Uchida.
    • Journal Title

      General Medicine

      Volume: 16 Issue: 2 Pages: 103-106

    • DOI

      10.14442/general.16.103

    • NAID

      130005078484

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anemia mmanegement and morality risk in newly visiting patients with chronic kidney disease in Japan: The CKD-ROUTE study.2015

    • Author(s)
      Soichiro Iimori, Shotaro Naito, Yumi Noda, Hidenori Nishida, Naofumi Yui, Tomokazu Okado, Sei Sasaki, Shinichi Uchida, Tatemitsu Rai.
    • Journal Title

      Nephrology

      Volume: 20 Issue: 9 Pages: 601-608

    • DOI

      10.1111/nep.12493

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] アクアポリン2の細胞内輸送調節における複合的リン酸化制御機構の解明2017

    • Author(s)
      油井直史、佐々木成、内田信一
    • Organizer
      日本腎臓学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Quantification of singleand multi-site AQP2 phosphorylation2017

    • Author(s)
      Yui N, Uchida S.
    • Organizer
      51th Annual Meeting of American Society of Nephrology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規腎臓線維化改善薬としてのbortezomibの検討2017

    • Author(s)
      銭谷慕子、森崇寧、油井直史、野村尚弘、千賀宗子、高橋大栄、蘇原映誠、頼建光、内田信一
    • Organizer
      日本腎臓学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Regulated Ser-261 dephosphorylation combined with Ser-256 and Ser-269 phosphorylation in the C-terminus of aquaporin-2 water channel2016

    • Author(s)
      Naofumi Yui, Sei Sasaki, Shinichi Uchida
    • Organizer
      50th Annual Meeting of American Society of Nephrology
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2016-11-15
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The proteasome inhibitor bortezomib attenuate renal fibrosis in mice2016

    • Author(s)
      Moko Zeniya, Takayasu Mori, Naohiro Nomura, Naofumi Yui, Eisei Sohara, Tatemitsu Rai, Shinichi Uchida
    • Organizer
      50th Annual Meeting of American Society of Nephrology
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2016-11-15
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] カルシニューリンは腎性尿崩症治療の標的分子である2016

    • Author(s)
      安藤史顕、蘇原映誠、油井直史、野村尚弘、菊池絵梨子、高橋大栄、森崇寧、頼建光、佐々木成、内田信一
    • Organizer
      第59回日本腎臓学会学術総会
    • Place of Presentation
      パシフィコ横浜 横浜 神奈川
    • Year and Date
      2016-06-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Dephosphorylation at Ser-261 is a determinant for the regulated AQP2 apical accumulation2015

    • Author(s)
      Naofumi Yui, Sei Sasaki, Shinichi Uchida
    • Organizer
      American Society of Nephrology
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Sunitinib-induced nephrotic syndrome and acute kidney injury in a malignant insulinoma patient: a case report2015

    • Author(s)
      Shoda Wakana, Naofumi Yui, Shotaro Naito, Soichiro Iimori, Koichiro Susa, Takayasu Mori, Naohiro Nomura, Eisei Sohara, Tomokazu Okado, Tatemitsu Rai, Shinichi Uchida.
    • Organizer
      American Society of Nephrology
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] スニチニブによる腎症の一例2015

    • Author(s)
      正田若菜、油井直史、工藤篤、大橋敦希、井上祐一、飯盛聡一郎、須佐紘一郎、森崇寧、佐藤文絵、野村尚弘、桑名仁、内藤省太郎、蘇原映誠、岡戸丈和、頼建光、内田信一
    • Organizer
      日本腎臓学会東部学術大会
    • Place of Presentation
      東京ミッドタウン ホール&カンファレンス(東京都港区)
    • Year and Date
      2015-10-02
    • Related Report
      2015 Research-status Report
  • [Presentation] バソプレッシン刺激によるアクアポリン2の活性化はGABAb受容体情報伝達機構により調節を受ける2015

    • Author(s)
      谷田部淳一、高野梢、谷田部緑、油井直史、蘇原映誠、内田信一、渡辺毅、木村純子
    • Organizer
      日本腎臓学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-06-07
    • Related Report
      2015 Research-status Report
  • [Presentation] Ser-269リン酸化AQP2の細胞内局在の時系列変化2015

    • Author(s)
      油井直史、佐々木成、内田信一
    • Organizer
      日本腎臓学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-06-06
    • Related Report
      2015 Research-status Report
  • [Remarks] 東京医科歯科大学腎臓内科

    • URL

      http://www.tmd.ac.jp/grad/kid/

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Remarks] 東京医科歯科大学 腎臓内科学

    • URL

      http://www.tmd.ac.jp/grad/kid/kid-J.htm

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2022-01-24  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi