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Rapid diagnosis of aqcuired Creutzfeldt-jakob disease with protein misfolding cyclic anplification

Research Project

Project/Area Number 15K09307
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurology
Research InstitutionTohoku University

Principal Investigator

Takeuchi Atsuko  東北大学, 医学系研究科, 助教 (00535239)

Co-Investigator(Renkei-kenkyūsha) Kitamoto Tetsuyuki  東北大学, 医学系研究科, 教授 (20192560)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsプリオン / クロイツフェルト・ヤコブ病 / 試験管内増幅 / 獲得型CJD / 医原性プリオン病 / PMCA / in vitro / プリオン病 / 医原性クロイツフェルトヤコブ病
Outline of Final Research Achievements

Plaque-type dura mater graft-associated Creutzfeldt-Jacob disease (p-dCJD) prions showed distinct amplification features from those of non p-dCJD (np-dCJD) prions in protein misfolding cyclic amplification (PMCA). Although no amplification from np-dCJD prions was observed with 129M or 129V substrates, p-dCJD prions were amplified drastically with the 129V substrates but not with the 129M substrates despite the PRNP codon 129 incompatibility between seed and substrate. Moreover, we found that type 2 PMCA products were newly generated from p-dCJD prions using type 2 PrPSc-specific antibody, while PrPSc in p-dCJD cases did not react with the type 2 PrPSc-specific antibody. These findings suggest that our PMCA is a useful tool for easily and rapidly identifying acquired CJD associated with the transmission of the V2 CJD strain of codon 129 methionine homozygotes, based on the preference for the 129 V substrate and the type of the amplified products.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Application of protein misfolding cyclic amplification for the rapid diagnosis of acquired Creutzfeldt-Jakob disease.2017

    • Author(s)
      Takeuchi A, Kobayashi A, Morita M, Kitamoto T
    • Journal Title

      Medical Research Archives

      Volume: 5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of protein misfolding cyclic amplification for the rapid diagnosis of acquired Creutzfeldt-Jakob disease2017

    • Author(s)
      Takeuchi A, Kobayashi A, Morita M, Kitamoto T
    • Journal Title

      Medical Research Archives

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Distinctive properties of plaque-type dura mater graft-associated Creutzfeldt-Jakob disease in cell-protein misfolding cyclic amplification2016

    • Author(s)
      Atsuko Takeuchi, Atsushi Kobayashi, Piero Parchi, Masahito Yamada, Masanori Morita, Shusei Uno and Tetsuyuki Kitamoto
    • Journal Title

      Laboratory Investigation

      Volume: in press Issue: 5 Pages: 581-7

    • DOI

      10.1038/labinvest.2016.27

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Diagnosis of MMiK type acquired Creutzfeldt-Jakob disease with cell- protein misfolding amplification (PMCA)2017

    • Author(s)
      Takeuchi A, Kobayashi A, Morita M, Kitamoto T
    • Organizer
      日本認知症学会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of the origin of Creutzfeldt-Jakob disease after cadaver-sourced pituitary growth hormone treatment using an amplification property in protein misfolding cyclic amplificatiton2016

    • Author(s)
      Takeuchi A, Yamamoto M, Parchi P, HaiK S, Morita M, Kobayashi A, Kitamoto T
    • Organizer
      PRION2016
    • Place of Presentation
      学術総合センター(東京)
    • Year and Date
      2016-05-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] cell-PMCA of acquired Creutzfeldt-Jakob disease2015

    • Author(s)
      Atsuko Takeuchi, Atsushi Kobayashi, Piero Parchi, Masahito Yamada, Masanori Morita, Shuusei Uno and Tetsuyuki Kitamoto
    • Organizer
      Asia Pacific Society of Prion Research
    • Place of Presentation
      金沢 (石川県立音楽堂)
    • Year and Date
      2015-09-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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