| Project/Area Number |
15K09322
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Neurology
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
Tokuda Takahiko 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80242692)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
建部 陽嗣 京都府立医科大学, 医学(系)研究科(研究院), 助教 (00637027)
渡邊 義久 京都府立医科大学, 医学(系)研究科(研究院), 講師 (50363990)
笠井 高士 京都府立医科大学, 医学(系)研究科(研究院), 講師 (70516062)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | α-シヌクレイン / オリゴマー / パーキンソン病 / 脂質 / エクソソーム / リポ蛋白 / 分子篩クロマトグラフィー / シヌクレインオリゴマー |
|---|
| Outline of Final Research Achievements |
It is known that extracellular α-synuclein (A-syn) oligomers are important as a pathogenic agent in Parkinson’s disease. In this research project, we first analyzed A-syn molecules in human plasma using gel-filtration chromatography. A-syn molecules in plasma were found in fraction with a molecular mass of 60 kDa and ~2,000 kDa. Furthermore, analyses of human plasma using lipid-adsorption columns demonstrated that A-syn molecules associated with lipids exist in the fractions corresponding to HDLs, and co-exist with ApoA1, ApoE, and ApoJ molecules. Some of those HDL-associated A-syn molecules were detected as oligomers. Our studies revealed that A-syn molecules in human plasma are mainly associated with HDL particles, and at least some part of them are oligomerized.
|
