| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Outline of Final Research Achievements |
We had developed a new rat model, which has spontaneous seizure beginning around 7 weeks old. We investigated the pathogenesis of spontaneous seizure in relation to neuroimmunological aspect, especially to glial cells, using immune staining and PCR analysis. NMDAR1, NMDAR2A, NMDAR2B, GAD, GFAP, AQP4, Vimentin, Cx43, Cx36, Iba-I, Cx3cr1, IL-1R, IL1β, TLR4 were measured at 4 weeks and 8 weeks after birth. As a result, immune activation at the foot processes of microglia and astrocyte were presumed before onset of epilepsy. Based on the result, we speculated that the immune suppressive therapy targeting at IL-1, TLR4 before disease onset may prevent the development of epileptogenesis. In other experiment, we were able to extract the change of functional network in epilepsy patients by measuring the auditory evoked magnetic field. The change of auditory evoked magnetic response is expected to be a biomarker of epilepsy severity.
|
