Significance of simultaneous secretion of insulin and incretin regulated by SKIP molecule
| Project/Area Number |
15K09384
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HOSODA Kiminori 京都大学, 大学院医学研究科, 教授 (40271598)
TOMITA Tsutomu 京都大学, 大学院医学研究科, 助教 (50402897)
MASHIMO Tomoji 京都大学, 大学院医学研究科, 准教授 (80397554)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | インスリン分泌 / インクレチン分泌 / 糖尿病 / グルコース応答性 / インスリン顆粒 / 惹起経路 / 増幅経路 |
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| Outline of Final Research Achievements |
We find here that sphingosine kinase 1-interacting protein (SKIP, also called Sphkap) is highly expressed in pancreatic b-cells but not in a-cells. Intraperitoneal glucose tolerance test showed that plasma glucose levels were decreased and insulin levels were increased in SKIP-/- mice compared to SKIP+/+ mice, but exendin-4-enhanced insulin secretion was masked. GSIS was amplified more in SKIP-/- but exendin-4-enhanced insulin secretion was masked compared to that in SKIP+/+ islets. The ATP and cAMP content were similarly increased in SKIP+/+ and SKIP-/- islets; depolarization-evoked, PKA and cAMP-mediated insulin secretion were not affected. Inhibition of PDE activity equally augmented GSIS in SKIP+/+ and SKIP-/- islets. These results indicate that SKIP modulates GSIS by a pathway distinct from that of cAMP-, PDE- and sphingosine kinase-dependent pathways.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Sitagliptin monotherapy has better effect on insulinogenic index than glimepiride monotherapy in Japanese patients with type 2 diabetes mellitus: a 52-week, multicenter, parallel-group randomized controlled trial.2016
Author(s)
Kondo Y, Harada N, Hamasaki A, Kaneko S, Yasuda K, Ogawa E, Harashima S, Yoneda H, Fujita Y, Kitano N, Nakamura Y, Matsuo F, Shinji M, Hinotsu S, Nakayama T, Inagaki N
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Journal Title
Diabetol Metab Syndr.
Volume: 8
Issue: 1
Pages: 1-5
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ayano-Takahara S, Ikeda K, Fujimoto S, Asai K, Oguri Y, Harashima S, Tsuji H, Shide K, Inagaki N.2015
Author(s)
Ayano-Takahara S, Ikeda K, Fujimoto S, Asai K, Oguri Y, Harashima S, Tsuji H, Shide K, Inagaki N.
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Journal Title
J Diabetes Investig.
Volume: 6
Issue: 6
Pages: 678-686
DOI
Related Report
Peer Reviewed / Open Access
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