| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Pancreatic bud/epithelial cells (NKX6.1+ cells) are considered as pancreas-committed cells. Induction of the NKX6.1+ cell is a critical step for the generation of pancreatic cells from human pluripotent stem cells. However, the induction method and mechanism have not yet been fully established. In this study, we identified low molecular weight compounds which promote differentiation into NKX6.1+ cells from human iPS cells. Based on the analysis of the compounds, we found that cytoskeleton regulating molecules are involved in the part of the mechanisms. Our differentiation protocol established in this study and the mechanism may be useful for both basic research and clinical application using pancreatic cells.
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