The intrinsic roles of human PAI-1 in adipocyte differentiation and function with using patient-derived iPS cells.
Project/Area Number |
15K09411
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Metabolomics
|
Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
Sano Hideto 浜松医科大学, 医学部, 助教 (80623842)
|
Co-Investigator(Kenkyū-buntansha) |
浦野 哲盟 浜松医科大学, 医学部, 教授 (50193967)
鈴木 優子 浜松医科大学, 医学部, 准教授 (20345812)
田中 宏樹 浜松医科大学, 医学部, 助教 (50456563)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | PAI-1 / adipocytes / endothelial cells / iPS cells / iPS細胞 / 脂肪細胞 / 血管内皮細胞 / 内皮細胞 / 細胞分化 / 細胞内代謝 |
Outline of Final Research Achievements |
We have generated iPS cells (PAI-1 iPS) from PAI-1 deficient patients, then differentiated them into mature cells, and analyzed the intrinsic function of human PAI-1. We have attempted adipocyte differentiation from PAI-1 iPS. Mesenchymal stem cell (MSC) - like cells could be detected, but we are still inquiring into efficient condition of adipocyte differentiation. On the other hand, in the gene expression analysis of iPS cell-derived endothelial cells (iPS-ECs) which are already established, we observed the expression of hormone-like protein genes that involved in adipocyte differentiation such as IGFBP 3 and 5. Currently, this iPS-ECs are co-cultured with the above MSC-like or adipocyte precursor cell line such as 3T3-L1 cells, and confirmed PAI-1 functions and the effects on adipocyte differentiation.
|
Report
(4 results)
Research Products
(17 results)
-
-
[Journal Article] Elevated Plasma Levels of LDL Cholesterol Promote Dissecting Thoracic Aortic Aneurysms in Angiotensin II-Induced Mice.2018
Author(s)
Tanaka H, Iida Y, Iwaki T, Suzuki Y, Sano H, Miyajima C, Zaima N, Sasaki T, Sumioka A, Hakamata S, Shimizu H, Umemura K, Urano T.
-
Journal Title
Ann Vasc Surg.
Volume: 48
Pages: 204-213
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Lysophosphatidylcholine Acyltransferase-3 Expression Is Associated with Atherosclerosis Progression.2017
Author(s)
Tanaka H, Zaima N, Sasaki T, Yamamoto N, Inuzuka K, Yata T, Iwaki T, Umemura K, Sano H, Suzuki Y, Urano T, Setou M, Unno N
-
Journal Title
J Vasc Res
Volume: 54(4)
Issue: 4
Pages: 200-208
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-