Understanding of the pathogenesis of thyroid disorder by studying the effect of colloidal Tg on gene regulation

• Project/Area Number: 15K09444
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Teikyo University
• Principal Investigator: Suzuki Koichi 帝京大学, 医療技術学部, 教授 (20206478)
• Research Collaborator: KAWASHIMA Akira ; ISHIDO Yuko ; LUO Yuqian ; YOSHIHARA Aya ; ODA Kenzaburo ; USUKURA Kensei
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 甲状腺 / サイログロブリン / 甲状腺ホルモン / 遺伝子発現

Outline of Final Research Achievements

We have uncovered that high concentration of thyroglobulin accumulated in the follicular lumen of the thyroid has two distinct activity: one is to suppress gene expressions necessary for biosynthesis of thyroid hormone and iodine transport and the other is rather to enhance colloid resorption, hydrolysis, transport, and secretion. This evidence indicates that follicular thyroglobulin is the intrinsic autoregulator of thyroid hormone biosynthesis, its storage and secretion by negative-feedback mechanisms. It is speculated that gain-of-function of this mechanism will lead to hypothyroidisms and loss-of-function will induce hyperthyroidisms.

Research Products

• [Journal Article] Follicular thyroglobulin induces cathepsin H expression and activity in thyrocytes. (2017)
  • Author(s): Oda K, Luo Y, Yoshihara A, Ishido Y, Sekihata K, Usukura K, Sue M, Hiroi N, Hirose T, Suzuki K.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 483 Pages: 541-546
  • DOI: 10.1016/j.bbrc.2016.12.109
  • Peer Reviewed / Open Access
• [Journal Article] A Novel Role for Flotillin-Containing Lipid Rafts in Negative-Feedback Regulation of Thyroid-Specific Gene Expression by Thyroglobulin. (2016)
  • Author(s): Luo Y, Akama T, Okayama A, Yoshihara A, Sue M, Oda K, Hayashi M, Ishido Y, Hirano H, Hiroi N, Katoh R, Suzuki K.
  • Journal Title: Thyroid Volume: 26 Pages: 1630-1639
  • DOI: 10.1089/thy.2016.0187
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Follicular thyroglobulin enhances gene expression necessary for thyroid hormone secretion (2015)
  • Author(s): Ishido Y, Luo Y, Yoshihara A, Hayashi M, Yoshida A, Hisatome I and Suzuki K
  • Journal Title: Endocr J Volume: 62 Issue: 11 Pages: 1007-1015
  • DOI: 10.1507/endocrj.ej15-0263 10.1507/endocrj.EJ15-0263
  • NAID: 130005109819
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Presentation] 濾胞内サイログロブリンは甲状腺細胞におけるカテプシンHの発現および活性を誘導する (2017)
  • Author(s): 小田健三郎、Luo Yuqian、吉原彩、石藤雄子、関端健吾、臼倉健世、廣井直樹、弘世貴久、鈴木幸一
  • Organizer: 第33回甲状腺病態生理研究会
• [Presentation] A novel role for flotillin-containing lipid rafts in negative-feedback regulation of thyroid-specific gene expression by thyroglobulin (2017)
  • Author(s): Yuqian Luo, Takeshi Akama, Akiko Okayama, Yuko Ishido, Hisashi Hirano, and Koichi Suzuki
  • Organizer: 99th Annual Meeting and Expo of the Endocrine Society (ENDO 2017)
  • Int'l Joint Research
• [Presentation] Follicular thyroglobulin induces cathepsin H expression and activity in the thyrocytes (2017)
  • Author(s): Kenzaburo Oda, Yuqian Luo, Aya Yoshihara, Yuko Ishido, Kengo Sekihata, Kensei Usukura, Mariko Sue, Naoki Hiroi, Takahisa Hirose, Koichi Suzuki
  • Organizer: 99th Annual Meeting and Expo of the Endocrine Society (ENDO 2017)
  • Int'l Joint Research
• [Presentation] 甲状腺ホルモン合成におけるヨード代謝調節の検討 (2017)
  • Author(s): 吉原 彩、臼倉健世、Yuqian Luo、川島 晃、小田健三郎、石藤雄子、廣井直樹、鈴木幸一
  • Organizer: 第90回 日本内分泌学会学術総会
• [Presentation] コロイド内thyroglobulinによる濾胞機能autocrine negative feedback調節機構研究の展開 (2016)
  • Author(s): 鈴木幸一
  • Organizer: 第59回日本甲状腺学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2016-11-03
  • Invited
• [Presentation] サイログロブリン(Tg)による甲状腺機能遺伝子発現調節は細胞膜脂質ラフトのflotillinによって介在される (2015)
  • Author(s): 駱 予倩、赤間 剛、岡山 明子、石藤 雄子、平野 久、鈴木 幸一
  • Organizer: 第58回日本甲状腺学会
  • Place of Presentation: 福島市
  • Year and Date: 2015-11-05
• [Book] Adv Protein Chem Struct Biol: Chromatin Remodelling and Immunity (2017)
  • Author(s): Suzuki K and Luo Y
  • Total Pages: 999
  • Publisher: Elsevier, Kidlington, Oxford UK
• [Book] Encyclopedia of Cell Biology vol 1 (2016)
  • Author(s): Luo Y, Sellitti DF and Suzuki K
  • Total Pages: 11
  • Publisher: Academic Press