| Project/Area Number |
15K09445
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
MORI Kenji 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (00416223)
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| Co-Investigator(Renkei-kenkyūsha) |
HANADA Reiko 大分大学, 医学部, 教授 (00343707)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 生理活性ペプチド / 神経ペプチド / Gタンパク質共役型受容体 |
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| Outline of Final Research Achievements |
To search for novel bioactive peptide as endogenous ligand for orphan G protein-coupled receptor (GPCR), the novel assay system was introduced. This assay detected the activation of Gq protein-coupled receptor by quantifying inositol monophosphate which is a downstream metabolite of inositol triphosphate, and revealed the constitutive activities of several orphan GPCRs. These data suggest that the inverse agonists, in addition to agonists, may exist as endogenous ligand for these GPCRs.
To determine the molecular form of newly identified neuropeptide neuromedin U precursor-related peptide (NURP), immunoaffinity chromatography of tissue extracts was performed. In rat brain and small intestine, NURP was present as two mature peptides of 33 and 36 residues. Intracerebroventricularly administered NURP induced increase in plasma prolactin concentration by acting indirectly on the pituitary. Dopamine from the hypothalamus may be involved in this activity of NURP.
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