Challenge to establish new preventive and therapeutic methods for rheumatoid arthritis

• Project/Area Number: 15K09522
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: University of Toyama
• Principal Investigator: Tatsuhiko Ozawa 富山大学, 大学院医学薬学研究部(医学), 助教 (10432105)
• Co-Investigator(Renkei-kenkyūsha): KATO Ryuichi 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (50240833)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: モノクローナルACPA / 関節リウマチ / シトルリン化 / ISAAC法 / フィブリノーゲン / ACAP / CCP / シトルリン化タンパク質 / モノクローナル抗体

Outline of Final Research Achievements

We tried to identify for nondenaturing proteins recognized by CCP-Ab1, an autoantibody derived from rheumatoid arthritis patients. Our findings demonstrated that a monoclonal ACPA (CCP-Ab1) bound to fibrinogen under native conditions. Next, we made the amino acid sequences of CCP-Ab1 revert to their germline sequences (CCP-Ab1 GL-rev) and analyzed its binding activity to the fibrinogen. CCP-Ab1 GL-rev did not bound to fibrinogen. These results suggested that CCP-Ab1 was generated by the stimulation of B-cells with citrullinated denatured protein and it acquired the reactivity to fibrinogen during B-cell differentiation by somatic hypermutation.

Research Products

• [Journal Article] Ligand-activated epidermal growth factor receptor (EGFR) signaling governs endocytic trafficking of unliganded receptor monomers by non-canonical phosphorylation. (2018)
  • Author(s): Tanaka T, Zhou Y, Ozawa T, Okizono R, Banba A, Yamamura T, Oga E, Muraguchi A, Sakurai H.
  • Journal Title: J. Biol. Chem Volume: 293 Issue: 7 Pages: 2288-2301
  • DOI: 10.1074/jbc.m117.811299
  • Peer Reviewed
• [Journal Article] Identification of Tumoricidal TCRs from Tumor-Infiltrating Lymphocytes by Single-Cell Analysis (2018)
  • Author(s): Shitaoka Kiyomi、Hamana Hiroshi、Kishi Hiroyuki、Hayakawa Yoshihiro、Kobayashi Eiji、Sukegawa Kenta、Piao Xiuhong、Lyu Fulian、Nagata Takuya、Sugiyama Daisuke、Nishikawa Hiroyoshi、Tanemura Atsushi、Katayama Ichiro、Murahashi Mutsunori、Takamatsu Yasushi、Tani Kenzaburo、Ozawa Tatsuhiko、Muraguchi Atsushi
  • Journal Title: Cancer Immunology Research Volume: 6 Issue: 4 Pages: 378-388
  • DOI: 10.1158/2326-6066.cir-17-0489
  • Peer Reviewed / Open Access
• [Journal Article] Cisplatin-induced non-canonical endocytosis of EGFR via p38 phosphorylation of the C-terminal region containing Ser-1015 in non-small cell lung cancer cells (2018)
  • Author(s): Tanaka T, Ozawa T, Oga E, Muraguchi A, Sakurai H
  • Journal Title: Oncol Lett Volume: in press
  • Peer Reviewed
• [Journal Article] Surveillance Study of Influenza Occurrence and Immunity in a Wisconsin Cohort During the 2009 Pandemic (2017)
  • Author(s): Lo CY, Strobl SL, Dunham K, Wang W, Stewart L, Misplon JA, Garcia M, Gao J, Ozawa T, Price GE, Navidad J, Gradus S, Bhattacharyya S, Viboud C, Eichelberger MC, Weiss CD, Gorski J, Epstein SL
  • Journal Title: Open Forum Infect Dis Volume: 4 Issue: 2
  • DOI: 10.1093/ofid/ofx023
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Association Between High-Avidity T-Cell Receptors, Induced by α-Fetoprotein-Derived Peptides, and Anti-Tumor Effects in Patients With Hepatocellular Carcinoma (2017)
  • Author(s): Nakagawa H, Mizukoshi E, Kobayashi E, Tamai T, Hamana H, Ozawa T, Kishi H, Kitahara M, Yamashita T, Arai K, Terashima T, Iida N, Fushimi K, Muraguchi A, Kaneko S
  • Journal Title: Gastroenterology Volume: 152 Issue: 6 Pages: 1395-1406
  • DOI: 10.1053/j.gastro.2017.02.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of an HLA class I allele closely involved in the auto-antigen presentation in acquired aplastic anemia (2017)
  • Author(s): Zaimoku Y, Takamatsu H, Hosomichi K, Ozawa T, Nakagawa N, Imi T, Maruyama H, Katagiri T, Kishi H, Tajima A, Muraguchi A, Kashiwase K, Nakao S
  • Journal Title: Blood Volume: 印刷中 Issue: 21 Pages: 2908-2916
  • DOI: 10.1182/blood-2016-11-752378
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Novel Epitopes with Agonistic Activity for the Development of Tumor Immunotherapy Targeting TRAIL-R1. (2017)
  • Author(s): Guo L, Sun X, Hao Z, Huang J, Han X, You Y, Li Y, Shen M, Ozawa T, Kishi H, Muraguchi A, Jin A.
  • Journal Title: J Cancer. Volume: 8 Issue: 13 Pages: 2542-2553
  • DOI: 10.7150/jca.19918
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A novel, rapid and efficient method of cloning functional antigen-specific T-cell receptors from single human and mouse T-cells (2016)
  • Author(s): Hamana H, Shitaoka K, Kishi H, Ozawa T, Muraguchi A
  • Journal Title: Biochem Biophys Res Commun Volume: 474 Issue: 4 Pages: 709-714
  • DOI: 10.1016/j.bbrc.2016.05.015
  • Peer Reviewed
• [Journal Article] TRAIL-receptor 1 IgM antibodies strongly induce apoptosis in human cancer cells in vitro and in vivo. (2016)
  • Author(s): Piao X, Ozawa T, Hamana H, Shitaoka K, Jin A, Kishi H, Muraguchi A.
  • Journal Title: Oncoimmunology Volume: 5 Issue: 5 Pages: e1131380-e1131380
  • DOI: 10.1080/2162402x.2015.1131380
  • Peer Reviewed
• [Journal Article] Feedback control of ErbB2 via ERK-mediated phosphorylation of a conserved threonine in the juxtamembrane domain (2016)
  • Author(s): Kawasaki Y, Sakimura A, Park CM, Tomaru R, Tanaka T, Ozawa T, Zhou Y, Narita K, Kishi H, Muraguchi A, Sakurai H
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 31502-31502
  • DOI: 10.1038/srep31502
  • Peer Reviewed / Open Access
• [Journal Article] Fully human monoclonal antibodies to TRAIL-R1 enhance TRAIL-induced apoptosis via activation of caspase-8 pathway. (2016)
  • Author(s): Hao Z, Han X, Sun X, Shen M, Huang J, Li Y, Ozawa T, Pang D, Jin S, Kishi H, Muraguchi A, Jin A.
  • Journal Title: Biochem Biophys Res Commun. Volume: 475 Issue: 2 Pages: 238-44
  • DOI: 10.1016/j.bbrc.2016.05.089
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A Human Anti-M2 Antibody Mediates Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Cytokine Secretion by Resting and Cytokine-Preactivated Natural Killer (NK) Cells (2015)
  • Author(s): Venkateswara R. Simhadri, Milena Dimitrova, John L. Mariano, Olatz Zenarruzabeitia, Weimin Zhong, Tatsuhiko Ozawa, Atsushi Muraguchi, Hiroyuki Kishi, Maryna C. Eichelberger, Francisco Borrego
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0124677-e0124677
  • DOI: 10.1371/journal.pone.0124677
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay (2015)
  • Author(s): Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 11333-11333
  • DOI: 10.1038/srep11333
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Monoclonal Antibody Against Citrullinated Peptides Obtained From Rheumatoid Arthritis Patients Reacts With Numerous Citrullinated Microbial and Food Proteins (2015)
  • Author(s): Reina Tsuda, Tatsuhiko Ozawa, Eiji Kobayashi, Hiroshi Hamana, Hirofumi Taki, Kazuyuki Tobe, Eiji Sugiyama, Masahiro Iwamoto, Johji Imura, Hiroyuki Kishi, Atsushi Muraguchi
  • Journal Title: Arthritis & Rheumatology Volume: 67 Issue: 8 Pages: 2020-2031
  • DOI: 10.1002/art.39161
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 関節リウマチ患者由来自己抗体が認識する非変性状態の抗原の同定と自己抗体誘導仮説 (2017)
  • Author(s): 小澤 龍彦、津田玲奈、浜名洋、小林栄治、多喜博文、戸邊一之、村口 篤、岸 裕幸
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] Evolution of anti-citrullinated peptide antibody (ACPA): analysis of autoantigen under native condition using a human monoclonal ACPA (2017)
  • Author(s): Ozawa T, Tsuda R, Hamana H, Kobayashi E, Taki H, Tobe K, Muraguchi A, Kishi H.
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] The model of antigen detection system using yeast surface display library (2017)
  • Author(s): Ohnuki Y, Ozawa T, Hamana H, Kobayashi E, Lyu F, Muraguchi A, Kishi H
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] A novel method for the production of recombinant MHC/peptide complexes (2017)
  • Author(s): Lyu F, Ozawa T, Hamana H, Kobayashi E, Muraguchi A, Kishi H
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] Majority of tumor infiltrating lymphocytes in B16F10 melanoma recognized tumor-associated antigens,but not melanoma-associated antigens or neoantigens (2017)
  • Author(s): Shitaoka K, Hamana H, Kobayashi E, Ozawa T, Hayakawa Y, Muraguchi A, Kishi H
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] T cell receptor (TCR) gene cloning from tumor infiltrating lymphocytes of breast cancer patients towardTCR gene therapy (2017)
  • Author(s): Hamana H, Nagata T, Shitaoka K, Sukegawa K, Ozawa T, Kobayashi E, Muraguchi A, Kishi H
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] TCR repertoire analysis of antigen-specific T cells using immunospot array assay on a chip (T-ISAAC) technology (2017)
  • Author(s): Kobayashi E, Ozawa T, Hamana H, Lyu F, Muraguchi A, Kishi H
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] Production of Ro52/TRIM21 autoantibodies that react to PEP08 epitope in Ro52 clinically relate to themorbidity and severity of interstitial lung disease in connective tissue diseases (2017)
  • Author(s): Okumura M, Ozawa T, Taki H, Muraguchi A, Kishi H
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] 自己免疫性出血病XIII/13症例より作製したヒト単クローン抗体のエピトープ解析 (2017)
  • Author(s): 尾崎 司、惣宇利 正善、小澤 龍彦、村口 篤、一瀬 白帝
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] 乳がん浸潤リンパ球の迅速・簡便TCRレパートリー解析 (2017)
  • Author(s): 浜名 洋、長田 拓哉、下岡 清美、祐川 健太、小澤 龍彦、村口 篤、岸 裕幸
  • Organizer: 第22回がん免疫学会総会
• [Presentation] Novel Human Anti-HLA-Bw4 and B61 Monoclonal Antibodies Kill Malignant B Cells Via CDC/ADCC While Sparing Normal Peripheral Blood Cells (2017)
  • Author(s): Takamatsu H, Ozawa T, Zaimoku Y, Tsuji A, Hanamura I, Kuroda J, Tanaka H, Kawai S, Kishi H, Nakao S.
  • Organizer: ASH the 59th Annual Meeting and Exposition
  • Int'l Joint Research
• [Presentation] p38活性化はEGFR Ser-1015のリン酸化を介したエンドサイトーシスを誘導する (2017)
  • Author(s): 大賀 英司、田中 智大、小澤 龍彦、村口 篤、櫻井 宏明
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] CR repertoire of CD4+CD25+CD137+Foxp3+ tumor infiltrating lymphocytes in mice. (2016)
  • Author(s): Kishi H, Hamana H, Shitaoka K, Lyu F, Ozawa T, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター・ラグナガーデンホテル（沖縄県・宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] ingle-T-cell manipulation method using microwell array chip (T-ISAAC) allows rapid and efficient cloning of antigen-specific TCRs. (2016)
  • Author(s): Ozawa T, Kishi H, Hamana H, Tajiri K, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター・ラグナガーデンホテル（沖縄県・宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] A rapid and easy system for cDNAs cloning of antigen specific TCRs from single human and mouse T-cells within 4 days. (2016)
  • Author(s): Hamana H, Kishi H, Shitaoka K, Ozawa T, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター・ラグナガーデンホテル（沖縄県・宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] T cell receptor obtained from tumor-infiltrating lymphocytes without using tumor-specific antigens exhibits cytotoxicity to tumors in vitro and in vivo. (2016)
  • Author(s): Shitaoka K, Kishi H, Hamana H, Hayakawa Y, Ozawa T, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター・ラグナガーデンホテル（沖縄県・宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] CR repertoire analysis of OVA-specific T-cells infiltrating into OVA-expressing-melanoma during tumor progression. (2016)
  • Author(s): Lyu F, Shitaoka K, Hamana H, Hayakawa Y, Kishi H, Muraguchi A
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター・ラグナガーデンホテル（沖縄県・宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] Epitopes and tissue reactivity of monoclonal anti-TRIM21/Ro52 autoantibodies isolated from collagen disease-patients with interstitial lung disease. (2016)
  • Author(s): Okumura M, Ozawa T, Taki H, Kishi H, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター・ラグナガーデンホテル（沖縄県・宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] Peripheral myeloid dendritic cells may predict the prognosis of patients with hepatocellular carcinoma. (2016)
  • Author(s): Tajiri K, Ozawa T, Kishi H, Muraguchi A
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター・ラグナガーデンホテル（沖縄県・宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] evelopment of novel human anti-HLA-monoclonal antibodies for clinical applications using peripheral blood B cells derived from anti-HLA antibody-positive donors. (2016)
  • Author(s): Takamatsu H, Zaimoku Y, Ozawa T, Kawai S, Tanaka H, Kishi H, Muraguchi A, Nakao S.
  • Organizer: 58th ASH Annual Meeting & Exposition
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] ISAAC法を用いた抗原特異的T細胞検出法の開発 (2016)
  • Author(s): 小澤龍彦，岸裕幸，浜名洋，田尻和人，呂福蓮，村口篤
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-11-30
• [Presentation] ErbB2/ErbB3ヘテロ二量体におけるERKを介したErbB2 Thr-677リン酸化によるフィードバック制御 (2016)
  • Author(s): 河崎優希，崎村綾香，パクチョルミン，都丸里佳，小澤龍彦，周越，成田香織，村口篤，櫻井宏明
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 関節リウマチ患者由来モノクローナル抗CCP抗体の取得と抗原の解析 (2016)
  • Author(s): 岸裕幸，津田玲奈，小澤龍彦，村口篤
  • Organizer: 第28回日本神経免疫学会学術集会
  • Place of Presentation: 長崎ブリックホール（長崎県・長崎市）
  • Year and Date: 2016-09-29
  • Invited
• [Presentation] Rapid generation of monoclonal antibodies using lymphocyte-microarray chip (2016)
  • Author(s): Ozawa T, Kishi H., and Muraguchi A
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: ANAクラウンプラザホテル富山・富山国際会議場（富山県・富山市）
  • Year and Date: 2016-09-27
  • Invited
• [Presentation] Cis-interaction of TCRs and antigen/MHC class I complexes on CD8+ T cells causes their activation (2016)
  • Author(s): Kishi H, Jin A, Hamana H, Shitaoka K, Tajiri K, Kobayashi E, Kusano S, Yokoyama S, Ozawa T, Nagai T, Obata T, Hatakeyama S, Horii M, Hu Y, Zhang F, Muraguchi A.
  • Organizer: 2016 International Congress of Immunology
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] A rapid and efficient single-cell manipulation method using microwell array chip (ISAAC) technology for screening antigen-specific cytokines-secreting T-cells. (2016)
  • Author(s): Ozawa T, Kishi H, Hamana H, Tajiri K, Lyu F, Muraguchi A.
  • Organizer: 2016 International Congress of Immunology
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] A rapid and easy system for screening of antigen-specific TCRs. (2016)
  • Author(s): Hamana H, Kishi H, Shitaoka K, Piao X, Ozawa T, Muraguchi A.
  • Organizer: 2016 International Congress of Immunology
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Identification of tumor-specific T cell receptors of primary tumor-infiltrating lymphocytes (TILs) from B16F10 melanoma-bearing mice at single cell levels. (2016)
  • Author(s): Shitaoka K, Hamana H, Kishi H, Ozawa T, Muraguchi A
  • Organizer: 2016 International Congress of Immunology
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Establishment of West Nile virus-neutralizing human monoclonal antibodies derived from the individuals vaccinated with inactivated Japanese encephalitis virus by ISAAC technology. (2016)
  • Author(s): Masaki H., Ozawa T., Takasaki T., Aoyama I., Yumisashi T., Konishi E., Kishi H., and Muraguchi A.
  • Organizer: 2016 International Congress of Immunology
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] B16F10メラノーマ浸潤リンパ球の単一細胞解析による，がん特異的T細胞の同定およびTCR遺伝子治療の試み (2016)
  • Author(s): 下岡清美，浜名洋，呂福蓮，小澤龍彦，早川芳弘，岸裕幸，村口篤
  • Organizer: 第20回日本がん免疫学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府・大阪市）
  • Year and Date: 2016-07-27
• [Presentation] ISAAC法を用いた関節リウマチ患者由来自己抗体の単離とその自己抗体が認識する新しい自己抗原の同定 (2015)
  • Author(s): 小澤龍彦、津田玲奈、浜名洋、多喜博文、戸邊一之、杉山英二、岩本雅弘、井村穣二、岸裕幸、村口篤
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県、神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 抗膜タンパク質ウサギ高親和性モノクローナル抗体のエピトープ配列を由来とする新規アフィニティタグの開発 (2015)
  • Author(s): 矢野智也、竹田浩之、小澤龍彦、村口篤、澤崎達也
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県、神戸市）
  • Year and Date: 2015-12-01
• [Presentation] コムギ無細胞系を用いた膜タンパク質間トランス相互作用解析技術 (2015)
  • Author(s): 栄谷紘一、竹田浩之、小澤龍彦、村口篤、澤崎達也
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県、神戸市）
  • Year and Date: 2015-12-01
• [Presentation] ERKを介したErbB2 Thr-677リン酸化によるErbB2/ErbB3ヘテロ二量体のフィードバック阻害機構 (2015)
  • Author(s): 河崎優希、崎村綾香、Chulmin Park、周越、都丸里佳、成田香織、小澤龍彦、村口篤、櫻井宏明
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県、神戸市）
  • Year and Date: 2015-12-01
• [Presentation] ISAAC 法による日本脳炎ワクチン被接種者末梢血単核球からのヒト抗ウエストナイルウイルス中和モノクローナル抗体の樹立 (2015)
  • Author(s): Masaki, H., Ozawa, T., Takahashi, T., Aoyama, I. Yumisashi, T.,Konishi, E., Kishi, H., Muraguchi, A.
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場（福岡県、福岡市）
  • Year and Date: 2015-11-22
• [Presentation] ＩＳＡＡＣ法による日本脳炎ワクチン被接種者末梢血単核球からのヒト抗ウエストナイルウイルス中和モノクローナル抗体の樹立（第２報） (2015)
  • Author(s): 正木秀幸、小澤龍彦、高崎智彦、青山幾子、弓指孝博、小西英二、岸裕幸、村口篤
  • Organizer: 第22回トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 福岡国際会議場（福岡県、福岡市）
  • Year and Date: 2015-11-21
• [Presentation] Reactivity of a monoclonal anti-citrullinated protein antibody obtained from RA patient with numerous citrullinated antigens (2015)
  • Author(s): Ozawa, T., Tsuda, R., Hamana, H., Taki, H., Kishi, H., Muraguchi, A.
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Identification of tumor-specific T cell receptors from primary tumor-infiltrating lymphocytes (TILs) obtained from B16F10 melanoma-bearing mice (2015)
  • Author(s): Shitaoka, K., Kishi, H., Hamana, H. Ozawa, T. Muraguchi, A.
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌市）
  • Year and Date: 2015-11-18
• [Presentation] TRAIL Receptor-1 IgM Antibodies Strongly Induced Apoptosis in Cancer Cells (2015)
  • Author(s): Piao, X., Ozawa, T., Kishi, H., Shitaoka, K., Hamana, H., Muraguchi, A.
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Obtaining monoclonal TRIM21/Ro52 autoantibodies from collagen diseases patients with interstitial lung disease and reactivity of peptide library with these monoclonal autoantibodies. (2015)
  • Author(s): Obi, M. Ozawa, T. Taki, H. Kishi, H. Muraguchi, A.
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Establishment of West Nile virus-neutralizing human monoclonal antibodies derived from the individuals vaccinated with inactivated Japanese encephalitis virus by ISAAC technology (2nd.) (2015)
  • Author(s): Masaki, H. Ozawa, T. Takahashi, T. Kishi, H. Muraguchi, A.
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Development of a NFAT-luciferase reporter system for screening of antigen-specific TCRs using non-T cells (2015)
  • Author(s): Hamana, H. Kishi, H. Shitaoka, K. Piao, X. Ozawa, T. Muraguchi, A.
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道、札幌市）
  • Year and Date: 2015-11-18
• [Presentation] 乳がん細胞におけるErbB2 Thr-677 リン酸化によるErb2/ErbB3 ヘテロダイマーのフィードバック阻害機構 (2015)
  • Author(s): 河崎優希、崎村綾香、Chulmin Park、周越、都丸里佳、成田香織、小澤龍彦、村口篤、櫻井宏明
  • Organizer: 第74回日本癌学会学術集会
  • Place of Presentation: 名古屋国際会議場（愛知県、名古屋市）
  • Year and Date: 2015-10-08