The molecular epidemiology of respiratory viruses associated with asthma attacks A single-center observational study in Japan

• Project/Area Number: 15K09579
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Kyorin University
• Principal Investigator: TAKESHI SARAYA 杏林大学, 医学部, 准教授 (40549185)
• Co-Investigator(Kenkyū-buntansha): 木村 博一 群馬パース大学, 保健科学部, 教授 (20391807) ; 石井 晴之 杏林大学, 医学部, 教授 (30406970) ; 倉井 大輔 杏林大学, 医学部, 准教授 (60530665) ; 滝澤 始 杏林大学, 医学部, 教授 (80171578)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 気道ウイルス / 気管支喘息発作 / 季節性 / マイコプラズマ / 呼吸器ウイルス / 分子疫学 / ウイルス感染 / 呼気凝集液 / サイトカイン / 気道ウイルス感染 / ウイルス / 気管支喘息

Outline of Final Research Achievements

A total of 106 asthma attack patients enrolled in this study in both inpatient (n=49) and outpatient (n=57) settings. Among these, patients with virus alone (n=39), virus and bacterial (n=5), and bacterial alone (n=5) were identified. The frequency of virus-positive patients was significantly higher in inpatients (75.3%, n=33) than in outpatients (19.3%, n=11). Major VRIs included human rhinovirus (HRV) (n=24), human metapneumovirus (hMPV) (n=9), influenza virus (Inf-V) (n=8), and respiratory syncytial virus (RSV) (n=3) infections with seasonal variations. HRV-A and HRV-C were the most commonly detected viruses, with wide genetic divergence on phylogenetic analysis. Asthmatic exacerbations in adults are highly associated with VRIs such as HRV-A or HRV-C, hMPV, RSV, and Inf-V infections with seasonal variations and genetic divergence, but similar frequencies of VRIs occurred in asthma attack patients throughout the
seasons.

Academic Significance and Societal Importance of the Research Achievements

本研究により、通年性の気管支喘息発作に寄与する呼吸器ウイルスの全体像が明らかとなった。また原因となる呼吸器ウイルスには季節性が存在すること、従来より指摘されていたマイコプラズマニューモニエやクラミドフィラニューモニエなどの細菌感染の関与は認めなかった。
気管支喘息発作に最も影響を与えるウイルスは感冒の主たるウイルスであるライノウイルスであり、分子遺伝学的には多様性を示していることが判明した。

Research Products

• [Journal Article] The molecular epidemiology of respiratory viruses associated with asthma attacks: A single-center observational study in Japan. (2017)
  • Author(s): Saraya T, Kimura H, Kurai D, Ishii H, Takizawa H.
  • Journal Title: Medicine (Baltimore). Volume: 96 Issue: 42 Pages: 1-7
  • DOI: 10.1097/md.0000000000008204
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] The molecular epidemiology of respiratory pathogens associated with asthma attacks: a single-center observational study in Japan (2018)
  • Author(s): Saraya T
  • Organizer: Joint Congress of the 7th Meeting of the Asian Organization for Mycoplasmology (7th AOM), The 45th Meeting of the Japanese Society of Mycoplasmology (45th JSM)
  • Int'l Joint Research
• [Presentation] The molecular epidemiology of respiratory pathogens associated with asthma attacks: a single-center observational study in Japan (2018)
  • Author(s): Takeshi Saraya
  • Organizer: Joint Congress of The 7th Meeting of the Asian Organization for Mycoplasmology (7th AOM), The 45th Meeting of the Japanese Society of Mycoplasmology (45th JSM)
  • Int'l Joint Research
• [Presentation] 気管支喘息発作におけるウイルス感染の前視方的研究 (2017)
  • Author(s): 皿谷 健、倉井 大輔、木村博一、石井晴之、滝澤 始
  • Organizer: 第57回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2017-04-21
• [Presentation] 成人気管支喘息発作・COPD増悪における 呼吸器ウイルス感染の頻度、臨床像の前向き観察研究 (2015)
  • Author(s): 皿谷 健
  • Organizer: 第８９回感染症学会総会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2015-04-16