The development of the new therapeutic drug which based on the structure of the virus activation enzyme: The new control method of the highly pathogenic infectious diseases.
| Project/Area Number |
15K09585
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Sagami Women's University |
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Principal Investigator |
Okumura Yuushi 相模女子大学, 栄養科学部, 教授 (70294725)
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| Co-Investigator(Kenkyū-buntansha) |
永野 ひかる 大阪府立大学, 公私立大学の部局等, 研究員 (10748924)
嶋田 昌子 相模女子大学, 栄養科学部, 教授 (30637369)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 高病原性ウイルス感染症 / ウイルス活性化酵素 / 膜結合型プロテアーゼ / 酵素阻害剤 / 高病原性感染症 |
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| Outline of Final Research Achievements |
Cleavage of viral envelope glycoprotein, hemagglutinin (HA), by host cellular proteases is essential step for influenza virus to enter into the target cells. We identified that ubiquitous type II transmembrane serine proteases, MSPL and its splice variant TMPRSS13, were candidates of HA-processing proteases of diverse highly pathogenic avian influenza (HPAI) viruses. In addition, we succeeded to reveal the crystal structure of MSPL/TMPRSS13. In this study, based on their structure, we first generated specific inhibitors for MSPL/TMPRSS13. To confirm the involvement of these proteases in HPAI virus infection, highly virulent virus (A/Crow/Kyoto/53/2004 (H5N1)) was infected into MSPL/TMPRSS13 stably expressed cells with or without their specific inhibitors. As a result, we concluded that these proteases specific inhibitors might be suppress HPAI virus multicycle replication and spreading. In vivo infectious experiments using their specific inhibitors are currently being investigated.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Structural analysis of the TKB domain of ubiquitin ligase Cbl-b complexed with its small inhibitory peptide, Cblin2016
Author(s)
Ohno A, Ochi A, Maita N, Ueji T, Bando A, Nakao R, Hirasaka K, Abe T, Teshima-Kondo S, Nemoto H, Okumura Y, Higashibata A, Yano S, Tochio H, Nikawa T
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Journal Title
Arch Biochem Biophys
Volume: 594
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] UCP3 is associated with Hax-1 in mitochondria in the presence of calcium ion2016
Author(s)
Hirasaka K, Mills EM, Haruna M, Bando A, Ikeda C, Abe T, Kohno S, Nowinski SM, Lago CU, Akagi K, Tochio H, Ohno A, Teshima-Kondo S, Okumura Y, Nikawa T
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Journal Title
Biochem Biophys Res Commun
Volume: 472
Issue: 1
Pages: 108-113
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Capric Acid Up-Regulates UCP3 Expression without PDK4 Induction in Mouse C2C12 Myotubes2016
Author(s)
Abe T, Hirasaka K, Kohno S, Tomida C, Haruna M, Uchida T, Ohno A, Oarada M, Teshima-Kondo S, Okumura Y, Choi I, Aoyama T, Terao J, Nikawa T*.
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Journal Title
Journal of Nutritional Science and Vitaminology
Volume: 62
Issue: 1
Pages: 32-39
DOI
NAID
ISSN
0301-4800, 1881-7742
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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