Proteomic analysis for drug-resistant mechanisms of Staphylococcus aureus

Research Project

Project/Area Number	15K09586
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Infectious disease medicine
Research Institution	Showa Pharmaceutical University (2017) St. Marianna University School of Medicine (2015-2016)
Principal Investigator	KANAMOTO Taisei 昭和薬科大学, 薬学部, 教授 (20260755)
Co-Investigator(Kenkyū-buntansha)	浅井大輔聖マリアンナ医科大学, 医学部, 講師 (10423485) 有戸光美聖マリアンナ医科大学, 医学部, 講師 (00509911)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	黄色ブドウ球菌 / 薬剤耐性 / プロテオミクス / 薬剤耐性機構 / MRSA / 質量分析
Outline of Final Research Achievements	Drug-resistant bacteria are great concern in medicine around the world. To clarify the precise mechanism of the resistance, we attempted to establish de novo method which can analyze the bacterial proteomics efficiently and precisely. A conventional method of 2D-PAGE analysis revealed that the expression level of 46 proteins in methicillin-resistant Staphylococcus aureus (MRSA) varied significantly under the cultivation with high antibiotics (oxacillin) concentration. We are constructing a recombinant protein library of MRSA for the internal control of the de novo proteomic analysis.