Proteomic analysis for drug-resistant mechanisms of Staphylococcus aureus

• Project/Area Number: 15K09586
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Showa Pharmaceutical University (2017); St. Marianna University School of Medicine (2015-2016)
• Principal Investigator: KANAMOTO Taisei 昭和薬科大学, 薬学部, 教授 (20260755)
• Co-Investigator(Kenkyū-buntansha): 浅井 大輔 聖マリアンナ医科大学, 医学部, 講師 (10423485) ; 有戸 光美 聖マリアンナ医科大学, 医学部, 講師 (00509911)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 黄色ブドウ球菌 / 薬剤耐性 / プロテオミクス / 薬剤耐性機構 / MRSA / 質量分析

Outline of Final Research Achievements

Drug-resistant bacteria are great concern in medicine around the world. To clarify the precise mechanism of the resistance, we attempted to establish de novo method which can analyze the bacterial proteomics efficiently and precisely. A conventional method of 2D-PAGE analysis revealed that the expression level of 46 proteins in methicillin-resistant Staphylococcus aureus (MRSA) varied significantly under the cultivation with high antibiotics (oxacillin) concentration. We are constructing a recombinant protein library of MRSA for the internal control of the de novo proteomic analysis.

Research Products

• [Presentation] 黄色ブドウ球菌の病原性解明 網羅的タンパク定量からのアプローチ (2018)
  • Author(s): 川島汐織、千澤直孝、西中志唯、金本大成
  • Organizer: 昭和薬科大学第11回ハイテクリサーチセンター報告会