Evaluation of new type of vaccine strategy against infectious diseases
Project/Area Number |
15K09590
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Infectious disease medicine
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Shimizu Kanako 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (20391980)
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Co-Investigator(Kenkyū-buntansha) |
藤井 眞一郎 国立研究開発法人理化学研究所, その他, 研究員 (10392094)
山崎 哲 国立研究開発法人理化学研究所, その他, 研究員 (30392161)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | in vivo DC targeting / NKT / antibody / CD4Tfh / influenza / NKT細胞 / 樹状細胞 / CD4Tfh細胞 / 抗体 |
Outline of Final Research Achievements |
The goal of vaccines against infectious diseases is to induce long-term cellular and humoral immunity. Development of synthetic anti-viral vaccines that trigger CD4+ T cell-dependent B cell immune responses has been attempted. Here, we show, using a cellular vaccine carrying foreign protein antigen plus iNKT cell glycolipid antigen, designated as artificial adjuvant vector cells (aAVCs), that mature DCs in situ elicit not only ordinal antigen-specific CD4+ T cells, but also CD4+ Tfh and germinal center, resulted in inducing long-term antibody production. As a mechanism, CD4+ Tfh cells, but not Bcl6-/- CD4+ T or iNKTfh cells played an important role. To develop it for influenza infection, we established influenza hemagglutinin-carrying aAVC (aAVC-HA) and found that all the mice vaccinated with aAVC-HA were protected from influenza infection. Thus, the in vivo DC targeting therapy by aAVC would be useful for protection against viral infection.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Systemic DC activation modulates the tumor microenvironment and shapes the long-lived tumor-specific memory mediated by CD8+ T cells.2016
Author(s)
Shimizu K, Yamasaki S, Shinga J, Sato Y, Watanabe T, Ohara O, Kuzushima K, Yagita H, Komuro Y, Asakura M, Fujii S
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Journal Title
Cancer Res
Volume: 76(13)
Issue: 13
Pages: 3756-3766
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Systemic DC activation modulates the tumor microenvironment and shapes the long-lived tumor-specific memory mediated by CD8+ T cells2016
Author(s)
Kanako Shimizu, Satoru Yamasaki, Jun Shinga, Yusuke Sato, Takashi Watanabe, Osamu Ohara, Kiyotaka Kuzushima, Hideo Yagita, Yoshiko Komuro, Miki Asakura, and Shin-ichiro Fujii
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Journal Title
Cancer Research
Volume: in press
Related Report
Peer Reviewed / Acknowledgement Compliant
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