| Project/Area Number |
15K09590
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Infectious disease medicine
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
Shimizu Kanako 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (20391980)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
藤井 眞一郎 国立研究開発法人理化学研究所, その他, 研究員 (10392094)
山崎 哲 国立研究開発法人理化学研究所, その他, 研究員 (30392161)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | in vivo DC targeting / NKT / antibody / CD4Tfh / influenza / NKT細胞 / 樹状細胞 / CD4Tfh細胞 / 抗体 |
|---|
| Outline of Final Research Achievements |
The goal of vaccines against infectious diseases is to induce long-term cellular and humoral immunity. Development of synthetic anti-viral vaccines that trigger CD4+ T cell-dependent B cell immune responses has been attempted. Here, we show, using a cellular vaccine carrying foreign protein antigen plus iNKT cell glycolipid antigen, designated as artificial adjuvant vector cells (aAVCs), that mature DCs in situ elicit not only ordinal antigen-specific CD4+ T cells, but also CD4+ Tfh and germinal center, resulted in inducing long-term antibody production. As a mechanism, CD4+ Tfh cells, but not Bcl6-/- CD4+ T or iNKTfh cells played an important role. To develop it for influenza infection, we established influenza hemagglutinin-carrying aAVC (aAVC-HA) and found that all the mice vaccinated with aAVC-HA were protected from influenza infection. Thus, the in vivo DC targeting therapy by aAVC would be useful for protection against viral infection.
|
