Prediction of DiabetesMellitus by using with iPS cell technology

• Project/Area Number: 15K09625
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kumamoto University
• Principal Investigator: MATSUMOTO SHIROU 熊本大学, 大学院生命科学研究部(医), 准教授 (70467992)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA KIMITOSHI 熊本大学, 大学院生命科学研究部小児科学分野, 教授 (30336234) ; KIDO JUN 熊本大学, 医学部附属病院小児科学分野, 特任助教 (70721215)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 小児糖尿病 / iPS細胞 / エネルギー代謝 / 1型糖尿病 / β細胞 / 再生医療 / バイオマーカー / 糖尿病 / 細胞内代謝機能 / メタボローム解析

Outline of Final Research Achievements

We tried to establish for prediction of DiabetesMellitus by using with iPS cell technology and novel therapeutic strategy with stem cells. We could isolated iPS cells originated from healthy control and type 1 Diabetes Mellitus, which were differentiated into beta cells. We could establish the way to purify the insulin producing cells and expansion methods with these iPS cells. We could also establish the method of mass culture suitable to clinical therapy. In addition, we analyzed metabolome investigation in beta cells differentiated from iPS cells and it is indicated that mitochondrial dysfunction may influence type 1 diabetes.

Research Products

• [Journal Article] A significant difference in the blood carnitine values obtained by the enzymatic cycling and andem mass spectrometry methods (2018)
  • Author(s): Kido J, Inoue H, Suzuki Y, Tanaka M, Mitsubuchi H, Nakamura K, Endo F, Matsumoto S.
  • Journal Title: Clinical Lab. Volume: 64(1) Issue: 01+02/2018 Pages: 211-215
  • DOI: 10.7754/clin.lab.2017.170805
  • Peer Reviewed
• [Journal Article] Amelioration by glycine of brain damage in neonatal rat brain following hypoxia-ischemia (2017)
  • Author(s): Mori Hiroko、Momosaki Ken、Kido Jun、Naramura Tetsuo、Tanaka Kenichi、Matsumoto Shirou、Nakamura Kimitoshi、Mitsubuchi Hiroshi、Endo Fumio、Iwai Masanori
  • Journal Title: Pediatrics International Volume: 59 Issue: 3 Pages: 321-327
  • DOI: 10.1111/ped.13164
  • Peer Reviewed
• [Journal Article] Plasma exchange and chelator therapy rescues acute liver failure in Wilson disease without liver transplantation. (2017)
  • Author(s): Kido J, Matsumoto S, Momosaki K, Sakamoto R, Mitsubuchi H, Inomata Y, Endo F, Nakamura K
  • Journal Title: Hepatol Res. Volume: Mar;47(4) Issue: 4 Pages: 359-363
  • DOI: 10.1111/hepr.12711
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Perinatal diagnosis and amino acid profiles of a large family with ornithine transcarbamylase deficiency. (2018)
  • Author(s): Shirou Matsumoto, Jun Kido, Hiroshi Mitsubuchi, Fumio Endo and Kimitoshi Nakamura
  • Organizer: the International Conference on Ureagenesis Defects
  • Int'l Joint Research / Invited
• [Presentation] 小児生活習慣病患者における血中アミノ酸プロファイル (2017)
  • Author(s): 鈴木 陽輔、城戸 淳、黒澤 茶々、水洗添 泉、坂本 理恵子、中村 公 俊、松本 志郎、遠藤 文夫
  • Organizer: 日本アミノ酸学会第 11 回学術大会(JSAAS2017)
• [Presentation] MATI/III deficiency with demyelination of central tegmental tract during neonatal period. (2017)
  • Author(s): Shirou Matsumoto, Jun Kido, Endo Fumio, Kimitoshi nakamrua.
  • Organizer: The 12th Asia-Pacific Conference of Human Genetics.
  • Int'l Joint Research
• [Presentation] Drug development and in vitro modeling of Methylmalonic Acidemia (MMA) using patient specific induced pluripotinet stem cells (2017)
  • Author(s): Shirou Matsumoto, Tadahiro Numakawa, Takumi Era
  • Organizer: The 39th Annual Meeting of the Molecular Biology Society of Japan
  • Int'l Joint Research / Invited
• [Presentation] Induced pluripotent stem cell research: Basic Research for Development of New Drugs for Organic Acidemia. (2015)
  • Author(s): Shirou Matsumoto, Jun Kido, Rieko Sakamoto, Takumi Era, Fumio Endo
  • Organizer: The 3rd Annual Symposium of the Asian Society of Inherited Metabolic Disorders
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2015-11-12
  • Int'l Joint Research