Methylome Analysis of Thyroid Dysgenesis
| Project/Area Number |
15K09630
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | National Center for Child Health and Development (2016-2017)
Keio University (2015) |
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Principal Investigator |
Narumi Satoshi 国立研究開発法人国立成育医療研究センター, 分子内分泌研究部, 室長 (40365317)
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| Co-Investigator(Renkei-kenkyūsha) |
Ito Takashi 九州大学, 医学研究院, 教授 (90201326)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 小児内内分泌学 / 甲状腺形成異常 / 遺伝学 / エピジェネティクス / メチローム / 小児内分泌学 / メチル化異常 / 次世代シーケンシング / 先天性甲状腺機能低下症 |
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| Outline of Final Research Achievements |
In this study, we screened genomic region showing differential methylation levels using DNA samples derived from patients with thyroid dysgenesis (TD) or age-matched control individuals. Analysis with the PBAT method (TD N=7; control N=3) revealed about 300 genomi regions, including the CpG island of the thyroid-specific transcription factor PAX8, were identified as the candidate regions. However, replication analysis with the BeadArray method failed to reproduce the abnormalities identified in the initial screening. We also re-analyzed the difference with the dataset obtained by the BeadArray method, but no significant difference was detected in the clustering analysis and analysis focusing on thyroid-specific transcription factors. The above data did not support our working hypothesis that "DNA methylation abnormality is involved in the onset of TD".
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Report
(4 results)
Research Products
(7 results)
