| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this study, we screened genomic region showing differential methylation levels using DNA samples derived from patients with thyroid dysgenesis (TD) or age-matched control individuals. Analysis with the PBAT method (TD N=7; control N=3) revealed about 300 genomi regions, including the CpG island of the thyroid-specific transcription factor PAX8, were identified as the candidate regions. However, replication analysis with the BeadArray method failed to reproduce the abnormalities identified in the initial screening. We also re-analyzed the difference with the dataset obtained by the BeadArray method, but no significant difference was detected in the clustering analysis and analysis focusing on thyroid-specific transcription factors. The above data did not support our working hypothesis that "DNA methylation abnormality is involved in the onset of TD".
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