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Analysis of microRNAs related to the development of infantile atopic dermatitis

Research Project

Project/Area Number 15K09638
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pediatrics
Research InstitutionChiba University

Principal Investigator

Inoue Yuzaburo  千葉大学, 大学院医学研究院, 特任講師 (00456063)

Co-Investigator(Renkei-kenkyūsha) SHIMOJO Naoki  千葉大学, 大学院医学研究院, 教授 (40221303)
YAMAIDE Fumiya  千葉大学, 医学部附属病院, 助教 (50636199)
NAKANO Taiji  千葉大学, 医学部附属病院, 助教 (90708306)
Research Collaborator Dissanayake Eishika  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsアトピー性皮膚炎 / microRNA / 表皮角化細胞 / コホート研究 / 出生コホート / 皮膚角化細胞
Outline of Final Research Achievements

We found that the microRNA, hsa-miR-144-3p (miR-144), is highly expressed in the umbilical cord serum of infants who developed AD at 1 year of age. This change was not evident in maternal serum or at 1 year of age. The miR-144 levels did not correspond to the serum IgE levels at 1 year of age as well. Mir-144 targets ABCA1 and led to a decrease in ABCA1 levels and cholesterol accumulation in miR-144-transfected human primary epithelial keratinocytes. In response to house dust mite (HDM) stimulation, miR-144-transfected keratinocytes showed an increase in the translocation of the NF-κB p65 subunit. HDM stimulation also led to an increase in the mRNA levels of human beta defensin-2, which has been shown to promote mast cell degranulation, following miR-144 transfection of keratinocytes. These findings suggest that high levels of miR-144, compounded via NF-kB activation maybe an early atopy-independent trigger for the development of AD at 1 year of age.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] MicroRNAs in Allergic Disease2016

    • Author(s)
      Dissanayake E, Inoue Y
    • Journal Title

      Current Allergy and Asthma Reports

      Volume: 16 Issue: 9 Pages: 67-67

    • DOI

      10.1007/s11882-016-0648-z

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Serum microRNAs as novel biomarkers in childhood asthma2016

    • Author(s)
      Inoue Y, Dissanayake E, Nakano T, Kudo K, Misumi S, Ide T, Yamamoto T, Chiba K, Yamaide F, Yamaide A, Arima T, Tomiita M, Hoshioka A, Shimojo N.
    • Organizer
      The annual conference of KAPARD
    • Place of Presentation
      Muju, Korea
    • Year and Date
      2016-04-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Association between serum microRNA expression in maternal blood or in cord blood and the development of atopic dermatitis at one year of age2015

    • Author(s)
      Y. Inoue, T. Yamamoto, F. Yamaide, Y. Morita, T. Nakano, T. Arima, H. Kojima, N. Shimojo, S. Ochiai, A. Eguchi , C. Mori
    • Organizer
      第64回日本アレルギー学会学術大会
    • Place of Presentation
      グランドプリンスホテル新高輪(東京都・港区)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] 喘息の診断用マイクロRNA2015

    • Inventor(s)
      井上祐三朗
    • Industrial Property Rights Holder
      井上祐三朗
    • Industrial Property Rights Type
      特許
    • Patent Publication Number
      2017-038553
    • Filing Date
      2015-08-20
    • Acquisition Date
      2017-02-23
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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