Analysis of microRNAs related to the development of infantile atopic dermatitis
| Project/Area Number |
15K09638
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Chiba University |
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Principal Investigator |
Inoue Yuzaburo 千葉大学, 大学院医学研究院, 特任講師 (00456063)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIMOJO Naoki 千葉大学, 大学院医学研究院, 教授 (40221303)
YAMAIDE Fumiya 千葉大学, 医学部附属病院, 助教 (50636199)
NAKANO Taiji 千葉大学, 医学部附属病院, 助教 (90708306)
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| Research Collaborator |
Dissanayake Eishika
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | アトピー性皮膚炎 / microRNA / 表皮角化細胞 / コホート研究 / 出生コホート / 皮膚角化細胞 |
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| Outline of Final Research Achievements |
We found that the microRNA, hsa-miR-144-3p (miR-144), is highly expressed in the umbilical cord serum of infants who developed AD at 1 year of age. This change was not evident in maternal serum or at 1 year of age. The miR-144 levels did not correspond to the serum IgE levels at 1 year of age as well. Mir-144 targets ABCA1 and led to a decrease in ABCA1 levels and cholesterol accumulation in miR-144-transfected human primary epithelial keratinocytes. In response to house dust mite (HDM) stimulation, miR-144-transfected keratinocytes showed an increase in the translocation of the NF-κB p65 subunit. HDM stimulation also led to an increase in the mRNA levels of human beta defensin-2, which has been shown to promote mast cell degranulation, following miR-144 transfection of keratinocytes. These findings suggest that high levels of miR-144, compounded via NF-kB activation maybe an early atopy-independent trigger for the development of AD at 1 year of age.
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Report
(4 results)
Research Products
(4 results)
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[Presentation] Serum microRNAs as novel biomarkers in childhood asthma2016
Author(s)
Inoue Y, Dissanayake E, Nakano T, Kudo K, Misumi S, Ide T, Yamamoto T, Chiba K, Yamaide F, Yamaide A, Arima T, Tomiita M, Hoshioka A, Shimojo N.
Organizer
The annual conference of KAPARD
Place of Presentation
Muju, Korea
Year and Date
2016-04-09
Related Report
Int'l Joint Research / Invited
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[Presentation] Association between serum microRNA expression in maternal blood or in cord blood and the development of atopic dermatitis at one year of age2015
Author(s)
Y. Inoue, T. Yamamoto, F. Yamaide, Y. Morita, T. Nakano, T. Arima, H. Kojima, N. Shimojo, S. Ochiai, A. Eguchi , C. Mori
Organizer
第64回日本アレルギー学会学術大会
Place of Presentation
グランドプリンスホテル新高輪（東京都・港区）
Year and Date
2015-05-26
Related Report
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