| Project/Area Number |
15K09668
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Ruby Pawankar 日本医科大学, 医学部, 助教授 (00287674)
田辺 雄次郎 日本医科大学, 医学部, 助教 (00643818)
尾崎 優介 日本医科大学, 医学部, 助教 (60740561)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 腸内細菌叢 / 粘膜免疫 / 自然リンパ球 / 気管支喘息 / 乳児早期 / 抗菌薬 / 抗菌薬曝露による粘膜リンパ球の変化 / 抗菌薬曝露によるILC2の変化 / 抗菌薬曝露による腸内細菌構成変化 / 消化管と肺でのILC2の相違を評価 / 肺の病理像の相違を評価 / 2群での組織評価とフローサイトメトリー解析 / 粘膜自然リンパ球の評価方法の確立 / 粘膜評価方法の確立 |
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| Outline of Final Research Achievements |
We examined whether changes in mucosal immune cell profile, especially a profile of innate lymphoid cells, through altering the intestinal microbiota caused by antibiotic exposure, are associated with pathophysiology of asthma. First, we examined the effects of antibiotic exposure using Amoxicillin during infant on murine intestinal microbiota, we found that Amoxicillin exposure resulted in predominance of genus Bacteroides, with disappearance of genus Lactobacillus and genus Bifidobacterium. Also, we found decreased intestinal microbial diversity in Amoxicillin group compared with control group. Next, we examined mucosal immune cell profile in both intestine and lung, we found that profound decrease in mucosal immune cells and ILC2 cells in the intestine.
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