Analysis of the onset and curative mechanism of food allergy using multicolor flow cytometry.

• Project/Area Number: 15K09669
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Fujita Health University
• Principal Investigator: Tsuge Ikuya 藤田保健衛生大学, 医学部, 教授 (00231431)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 食物アレルギー / 経口免疫療法 / アレルゲン特異的T細胞 / 好塩基球 / フローサイトメトリー / 抗IgE抗体

Outline of Final Research Achievements

This study aimed to analyze the dynamics of allergen-specific type 2 helper T cells (Th2), regulatory T cells (Treg) and basophils in oral immunotherapy (OIT) participants to improve the treatment of food allergies.
After 4 months, patients receiving OIT using hypoallergenic peptide milk exhibited a marked decline in the number of IL-4 producing milk-specific T cells, implying OIT exerts its effects through the inhibition of Th2. In OIT combined with anti-IgE antibody therapy, the basophil activation test (BAT) was completely depressed by anti-IgE therapy only in a case where the succeeding OIT was effective. It might suggest that BAT is useful for determining the optimal dose of anti-IgE antibody and predicting its efficacy.

Research Products

• [Journal Article] Long-term immunological effects of Japanese cedar pollen-based subcutaneous immunotherapy (2018)
  • Author(s): Inuo C, Ando H, Tanaka K, Nakajima Y, Tsuge I, Urisu A, Kondo Y.
  • Journal Title: Allergology International Volume: 67 Issue: 3 Pages: 408-410
  • DOI: 10.1016/j.alit.2017.11.002
  • NAID: 130007420604
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access
• [Journal Article] The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction. (2017)
  • Author(s): Yamamura K, Uruno T, Shiraishi A, Tanaka Y, Ushijima M, Nakahara T, Watanabe M, Kido-Nakahara M, Tsuge I, Furue M, Fukui Y.
  • Journal Title: Nat Commun Volume: 8 Issue: 1 Pages: 13946-13946
  • DOI: 10.1038/ncomms13946
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 卵白未摂取アトピー性皮膚炎乳幼児に対するオボムコイド減量加熱全卵ベビーフードの安全性 (2017)
  • Author(s): 犬尾 千聡, 森 雄司, 近藤 康人, 田中 健一, 中島 陽一, 川井 学, 山脇 一夫, 木村 守, 柘植 郁哉, 宇理須 厚雄
  • Journal Title: 日本小児アレルギー学会誌 Volume: 31 Pages: 135-140
  • NAID: 130006832518
  • Peer Reviewed
• [Journal Article] Effect of Japanese cedar-specific sublingual immunotherapy on allergen-specific TH2 cell counts in blood. (2016)
  • Author(s): Nomura T, Suzuki M, Yokota M, Nakamura Y, Ozeki K, Ito Y, Tsuge I, Saitoh S.
  • Journal Title: Ann Allergy Asthma Immunol. Volume: 117 Issue: 1 Pages: 72-78
  • DOI: 10.1016/j.anai.2016.04.003
  • Peer Reviewed
• [Journal Article] IgE-dependent mechanism and successful desensitization of erythritol allergy. (2016)
  • Author(s): Sugiura S, Kondo Y, Tsuge I, Nakagawa T, Kando N, Ito K, Koyama N.
  • Journal Title: Ann Allergy Asthma Immunol. Volume: 117 Issue: 3 Pages: 320-321
  • DOI: 10.1016/j.anai.2016.06.019
  • Peer Reviewed
• [Journal Article] Multicolor flow-cytometric analysis of milk allergen-specific T-helper type 2 cells revealed coexpression of interleukin-4 with Foxp3. (2015)
  • Author(s): Yamawaki K, Inuo C, Nomura T, Tanaka K, Nakajima Y, Kondo Y, Yoshikawa T,
  • Journal Title: Ann Allergy Asthma Immunol. Volume: 115 Issue: 6 Pages: 503-8
  • DOI: 10.1016/j.anai.2015.09.020
  • Peer Reviewed
• [Journal Article] Japanese cedar pollen-based subcutaneous immunotherapy (2015)
  • Author(s): Inuo C, Kondo Y, Tanaka K, Nakajima Y, Nomura T, Ando H, Suzuki S, Tsuge I,
  • Journal Title: Int Arch Allergy Immunol. Volume: 167 Issue: 2 Pages: 137-45
  • DOI: 10.1159/000437325
  • Peer Reviewed / Open Access
• [Journal Article] Hen's Egg Allergy. (2015)
  • Author(s): Urisu A, Kondo Y, Tsuge I.
  • Journal Title: Chem Immunol Allergy. Volume: 101 Pages: 124-30
  • DOI: 10.1159/000375416
  • ISBN: 9783318023404, 9783318023411
• [Presentation] 低アレルゲン化エビ粉末の作成 (2017)
  • Author(s): 川井 学, 奥村 早希, 岡本 薫, 大高 早希, 森 雄司, 大久保 悠里子, 山脇 一夫, 中島 陽一, 高田 聡, 中村 厚, 近藤 智彦, 筬島 克裕, 板垣 康治, 近藤 康人, 柘植 郁哉
  • Organizer: 日本小児アレルギー学会
• [Presentation] 食物アレルギー難治症例に対する治癒を目指した取り組み 小児牛乳アレルギー児の経口免疫療法における抗原特異的T細胞の解析 (2016)
  • Author(s): 山脇 一夫, 柘植 郁哉, 森 雄司, 犬尾 千聡, 野村 孝泰, 田中 健一, 鈴木 聖子, 中島 陽一, 近藤 康人, 宇理須 厚雄
  • Organizer: 日本アレルギー学会
  • Place of Presentation: 東京
  • Year and Date: 2016-06-17
• [Presentation] 小児の牛乳アレルギー児における抗原特異的IL4産生Th2細胞とFoxp3発現の関連性の検討 (2015)
  • Author(s): 山脇 一夫, 柘植 郁哉, 森 雄司, 犬尾 千聡, 野村 孝泰, 田中 健一, 鈴木 聖子, 中島 陽一, 近藤 康人, 宇理須 厚雄
  • Organizer: 第64回日本アレルギー学会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-26
• [Presentation] 中島 陽一, 大高 早希, 森 雄司, 大久保 悠里子, 田中 健一, 山脇 一夫, 犬尾 千聡, 平田 典子, 鈴木 聖子, 近藤 康人, 柘植 郁哉, 宇理須 厚雄 (2015)
  • Author(s): 魚経口負荷試験242例の検討
  • Organizer: 第64回日本アレルギー学会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-26