| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Chronic granulomatous disease (CGD) is an inherited disorder caused by a mutation in the gene encoding gp91phox. The phagocytes of CGD patients lack the ability to produce superoxide anion (O2-) by the NAPDH oxidase complex enzyme. CGD patients are susceptible to severe infections. Cyt b, the catalytic center of the NADPH oxidase, consists of two subunits gp91phox and p22phox. It has been suggested that the extracellular region of gp91phox is necessary and sufficient to form the epitope for monoclonal antibody (mAb) 7D5. To further elucidate 7D5 epitope on human gp91phox, we constructed chimeric DNA expressed human and mouse gp91phox recombinant protein. The fusion proteins were immunostained for 7D5. The 143ELGDRQNES151 region was found to reside at the extracellular surface on human gp91phox and represents an important epitope for the interaction with 7D5. In particular, amino acid R147 is a unique epitope for membrane-associated Cyt b 7D5.
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