Search for biomarkers useful for discriminating bacterial pneumonia in children

• Project/Area Number: 15K09673
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: KUSUHARA Koichi 産業医科大学, 医学部, 教授 (20243941)
• Co-Investigator(Kenkyū-buntansha): 保科 隆之 産業医科大学, 医学部, 講師 (30398078) ; 石井 雅宏 産業医科大学, 医学部, 助教 (30461560)
• Co-Investigator(Renkei-kenkyūsha): KAMIZONO Junji 産業医科大学, 医学部, 非常勤医師 (50299616) ; MIYAKAWA Takayuki 産業医科大学, 医学部, 非常勤医師 (90219733)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 肺炎 / 喀痰 / 細菌性 / バイオマーカー / 鑑別 / 小児

Outline of Final Research Achievements

Subjects of this study were children admitted to the University of Occupational and Environmental Health Hospital for pneumonia from April 2015 to March 2018, whose sputum samples were suitable for detection of causative bacteria (median age, 3.1 years). When phagocytized bacterial cells were seen on the Gram stain smear of the sputum sample and corresponding bacterium was isolated later, it was identified as a bacterial pathogen. The patients with and without identification were classified into bacterial pneumonia group (n=19, 46%) and non-bacterial pneumonia group (n=22, 54%), respectively. White blood cell count, neutrophil count, CRP, procalcitonin, presepsin and monocyte/neutrophil CD64 expression on admission were compared between the 2 groups. No biomarkers evaluated in this study showed significant difference between them. In ROC analysis, neutrophil CD64 expression showed better ability to discriminate bacterial pneumonia.

Research Products

• [Journal Article] Effectiveness of an early switch from intravenous to oral antimicrobial therapy for lower respiratory tract infection in patients with severe motor intellectual disabilities. (2018)
  • Author(s): Kawamura M, Hoshina T, Ogawa M, Nakamoto T, Kusuhara K.
  • Journal Title: J Infect Chemother Volume: 24 Issue: 1 Pages: 40-44
  • DOI: 10.1016/j.jiac.2017.08.017
  • Peer Reviewed
• [Journal Article] Utility of the Performance of Lumbar Puncture for Febrile Children with Status Epilepticus in the Bacterial Conjugate Vaccine Era. (2018)
  • Author(s): Kaoru H, Igarashi R, Hoshina T, Kojiro M, Kusuhara K.
  • Journal Title: Pediatr Infect Dis J Volume: 印刷中 Issue: 10 Pages: 963-966
  • DOI: 10.1097/inf.0000000000001945
  • Peer Reviewed
• [Journal Article] Difference between Japanese Secondary and Tertiary Medical Facilities Regarding Changes in the Hospitalization of Children for Pneumonia after the Introduction of the Pneumococcal Conjugate Vaccine. (2017)
  • Author(s): Haro K, Ogawa M, Hoshina T, Kojiro M, Kusuhara K.
  • Journal Title: Jpn J Infect Dis Volume: 70 Pages: 270-274
  • NAID: 130005671856
  • Peer Reviewed / Open Access
• [Journal Article] The efficacy of the direct clinical intervention for infectious diseases by a pediatric infectious disease specialist in the pediatric ward of a tertiary medical facility without a pediatric antimicrobial stewardship program. (2017)
  • Author(s): Hoshina T, Yamamoto N, Ogawa M, Nakamoto T, Kusuhara K.
  • Journal Title: Eur J Clin Microbiol Infect Dis Volume: 36 Issue: 8 Pages: 1449-1454
  • DOI: 10.1007/s10096-017-2952-5
  • Peer Reviewed
• [Journal Article] Efficacy of periodic surveillance culture in a neonatal intensive care unit in the presumption of causative pathogens of late-onset bacterial infection. (2017)
  • Author(s): Ichikawa S, Hoshina T, Kinjo T, Araki S, Kusuhara K
  • Journal Title: Am J Infect Control Volume: 45 Issue: 3 Pages: 251-254
  • DOI: 10.1016/j.ajic.2016.09.022
  • Peer Reviewed
• [Journal Article] Difference of the change of the hospitalization for pneumonia in children between Japanese secondary and tertiary medical facilities after the introduction of pneumococcal conjugate vaccine. (2017)
  • Author(s): Haro K, Ogawa M, Hoshina T, Kojiro M, Kusuhara K
  • Journal Title: Jpn J Infect Dis Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Analysis of the Physiological Variation in Neutrophil CD64 Expression during the Early Neonatal Period. (2016)
  • Author(s): Miyake F, Ishii M, Hoshina T, Ichikawa S, Araki S, Kinjo T, Shibata E, Hachisuga T, Kusuhara K
  • Journal Title: Am J Perinatol Volume: 33 Pages: 1377-1381
  • Peer Reviewed
• [Journal Article] CCL2 level is elevated with metabolic syndrome and CXCL10 level is correlated with visceral fat area in obese children. (2016)
  • Author(s): Ishii M, Araki S, Goto M, Yamamoto Y, Kusuhara K
  • Journal Title: Endocr J Volume: 63 Pages: 795-804
  • NAID: 130005597974
  • Peer Reviewed
• [Journal Article] Serial Changes of Serum Cytokine in a Pediatric Patient with Severe Fever with Thrombocytopenia Syndrome. (2016)
  • Author(s): Kawase M, Hoshina T, Nakamoto T, Asai H, Ishii M, Kusuhara K.
  • Journal Title: Pediatr Infect Dis J Volume: 35 Issue: 3 Pages: 359-360
  • DOI: 10.1097/inf.0000000000001005
  • Peer Reviewed
• [Journal Article] Utility of ferritin as a predictor of the patients with Kawasaki disease refractory to intravenous immunoglobulin therapy. (2015)
  • Author(s): Yamamoto N, Sato K, Hoshina T, Kojiro M, Kusuhara K.
  • Journal Title: Mod Rheumatol. Volume: 25 Issue: 6 Pages: 898-902
  • DOI: 10.3109/14397595.2015.1038430
  • Peer Reviewed
• [Journal Article] The uselessness of procalcitonin in the diagnosis of focal bacterial central nervous system infection. (2015)
  • Author(s): Hoshina T, Takimoto T, Nanishi E, Nishio H, Kusuhara K, Hara T.
  • Journal Title: J Infect Chemother Volume: 21 Issue: 8 Pages: 620-622
  • DOI: 10.1016/j.jiac.2015.04.003
  • Peer Reviewed