Research on Pathogenesis of Acute Rupture of Chordae Tendinae of the Mitral Valve in Infants: Possible involvement of maternally derived anti-SSA antibody
| Project/Area Number |
15K09705
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
Shiraishi Isao 国立研究開発法人国立循環器病研究センター, 病院, 部長 (80295659)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIRAI Manabu 国立研究開発法人国立循環器病研究センター, 創薬オミックス解析センター, 室長 (70294121)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 僧帽弁腱索断裂 / 抗SSA抗体 / メタゲノム解析 / RNAトランスクリプトーム解析 / 川崎病 / 僧帽弁検索断裂 / 僧帽弁腱索 / 急性心不全 / DNA網羅的解析 / トランスクリプトーム解析 / 乳児 / 僧帽弁閉鎖不全 / SSA抗体 |
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| Outline of Final Research Achievements |
In order to elucidate the pathogenesis and pathophysiology of the mitral chordal rupture in infants, metagenome analysis was performed using a fresh mitral valve tissue of an infant with mitral chordal rupture. RNA transcriptome analysis was also performed using formalin fixed paraformaldehyde embedded (FFPE) mitral valve tissues taken from the 4 patients and 2 controls. So far, specific viral or bacterial genome has not been detected in metagenome analysis. Specific RNA up regulation and down regulation have not been identified including pathways concerning anti-SSA antibody, either.
To be able to obtain convincing results and elucidate pathogenesis and pathophysiology of the disease, metagenome and RNA transcriptome analysis with more samples are necessary.
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Report
(4 results)
Research Products
(4 results)
